Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,294,334 A→G 14.9% S218G (AGC→GGC)  PP_1126 → hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,294,3340AG14.9% 59.1 / 3.6 28S218G (AGC→GGC) PP_1126hydrolase
Reads supporting (aligned to +/- strand):  ref base A (13/10);  new base G (2/2);  total (16/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCCGCTGGACCAGGGCATGCGCGCCGGCCCGCCGATGCCCTGGAACCATGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCTGATTGATGCTTATGGCCAGTCGACTCGCGGTAACTGGCCCGAGAAGGTAT  >  NC_002947/1294202‑1294469
                                                                                                                                    |                                                                                                                                       
tCGCCGCTGGACCAGGGCATGCGCGCCGGCCCGCCGATGCCCTGGAACCATGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGcc                                                                                                                                      <  2:512080/136‑1 (MQ=255)
tCGCCGCTGGACCAGGGCATGCGCGCCGGCCCGCCGATGCCCTGGAACCATGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGcc                                                                                                                                      >  2:383008/1‑136 (MQ=255)
   ccgcTGGACCAGGGCATGCGCGCCGGCCCGCCGATGCCCTGGAACCATGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCa                                                                                                                                   <  1:235166/136‑1 (MQ=255)
            cAGGGCATGCGCGCCGGCCCGCCGATGCCCTGGAACCATGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGgcgc                                                                                                                          >  2:90960/1‑136 (MQ=255)
                 cATGCGCGCCGGCCCGCCGCTGCCCTGGAACCAAGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCCGCCCCAAGGTGGCGCAGAGc                                                                                                                     >  2:85400/1‑136 (MQ=255)
                            gCCCGCCGATGCCCTGGAACCATGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGa                                                                                                          >  1:292826/1‑136 (MQ=255)
                                    aTGCCCTGGAACCATGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCGGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCg                                                                                                  >  2:380880/1‑136 (MQ=255)
                                    aTGCCCTGGAACCATGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCGGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCg                                                                                                  >  2:378139/1‑136 (MQ=255)
                                               ccATGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCctgctg                                                                                                               <  1:208687/112‑1 (MQ=255)
                                               ccATGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCctgctg                                                                                                               >  2:208687/1‑112 (MQ=255)
                                                     tggtCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATgacgac                                                                                 >  2:519164/1‑136 (MQ=255)
                                                     tggtCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATgacgac                                                                                 >  2:259770/1‑136 (MQ=255)
                                                     tggtCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCACAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATgacgac                                                                                 <  1:383008/136‑1 (MQ=255)
                                                         cAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCACAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGACGGTATCCAGGATGACGACCAgg                                                                             >  2:534837/1‑136 (MQ=255)
                                                          aGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGc                                                                            >  1:110109/1‑136 (MQ=255)
                                                                    tttGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTTAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGggcg                                                                  >  1:363257/1‑136 (MQ=255)
                                                                         ccAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTa                                                             <  2:433409/136‑1 (MQ=255)
                                                                             cagcGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCAc                                                         <  2:339607/136‑1 (MQ=255)
                                                                                     gAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCGGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCtgat                                                 <  1:380880/136‑1 (MQ=255)
                                                                                     gAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCGGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCtgat                                                 <  1:378139/136‑1 (MQ=255)
                                                                                     gAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCtgat                                                 <  1:85400/136‑1 (MQ=255)
                                                                                              aCCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCTGATTGATGCTTa                                        >  2:364621/1‑136 (MQ=255)
                                                                                                   aaGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCTGATTGATGCTTATGGcc                                   >  1:391362/1‑136 (MQ=255)
                                                                                                           gACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCTGATTGATGCTTATGGCCAGTCGACt                           <  1:90960/136‑1 (MQ=255)
                                                                                                           gACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCTGATTGATGCTTATGGCCAGTCGACt                           <  1:240344/136‑1 (MQ=255)
                                                                                                            aCCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCTGATTGATGCTTATGGCCAGTCGACTc                          >  1:528212/1‑136 (MQ=255)
                                                                                                               tCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATgacga                                                                                  >  1:459093/1‑77 (MQ=255)
                                                                                                               tCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATgacga                                                                                  <  2:459093/77‑1 (MQ=255)
                                                                                                                                    aGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCTGATTGATGCTTATGGCCAGTCGACTCGCGGTAACTGGCCCGAGAAGGTAt  <  2:208634/136‑1 (MQ=255)
                                                                                                                                    aGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCTGATTGATGCTTATGGCCAGTCGACTCGCGGTAACTGGCCCGAGAAGGTAt  >  1:20799/1‑136 (MQ=255)
                                                                                                                                    |                                                                                                                                       
TCGCCGCTGGACCAGGGCATGCGCGCCGGCCCGCCGATGCCCTGGAACCATGGTGGTCAGCGCCTCCTTTTGACCAGCAGCGCCGGAGCAGTCGACCTCAAGCAGGTGACCTCGGTCAGCCTGAGCATCCCCAGCCCCAAGGTGGCGCAGAGCCTGCTGATCGAGCGTGTCGGTATCCAGGATGACGACCAGGCGTACAGGGCGGCCTACCACGAGCTGATTGATGCTTATGGCCAGTCGACTCGCGGTAACTGGCCCGAGAAGGTAT  >  NC_002947/1294202‑1294469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑