| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,930,569 | C→A | 21.0% | D42Y (GAC→TAC) | PP_1725 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,930,569 | 0 | C | A | 21.0% | 43.2 / 2.6 | 19 | D42Y (GAC→TAC) | PP_1725 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (9/6); new base A (2/2); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.53e-01 | |||||||||||
GTTGGTTGGACAGGCGGTTGATGTTGTTGTGCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGTGGCCCATCGCATGGTGGGCGACCTGATAGTTGAGGCCGTCGAGCAGGACCAGGATGACGTTGTGTTGCATGTTTTCTCCAGTCATGAACCGCGTTGGCACCTTCGCGGG > NC_002947/1930434‑1930701 | gttggttgGCCAGGCGGTTGAGGTTGTTGTGCACGATCCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTc < 1:443980/136‑1 (MQ=255)gttggttgGACAGGCGGTTGATGTTGTTGTGCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTc > 2:277543/1‑136 (MQ=255) ggACAGGCGGTTGATGTTGTTGTGCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTAGGCCTCg > 1:185740/1‑136 (MQ=255) ggACAGGCGGTTGATGTTGTTGTGCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTAGGCCTCg > 1:185930/1‑136 (MQ=255) gACAGGCGGTTGATGTTGTTGTGCACGATGCCGCTGTCGATAGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCAGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGa < 2:108677/136‑1 (MQ=255) ggCGGTTGATGTTGTTGTGCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTa > 1:284256/1‑136 (MQ=255) gTTGATGTTGTTGTGCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCg > 2:114934/1‑136 (MQ=255) tGATGTTGTTGTGCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTAGGCCTCGACGTAGGCGTg < 2:185740/136‑1 (MQ=255) tGATGTTGTTGTGCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTAGGCCTCGACGTAGGCGTg < 2:185930/136‑1 (MQ=255) ttgttgtGCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGt < 2:474567/136‑1 (MQ=255) gtgCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGTGGccc > 1:164918/1‑136 (MQ=255) gCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGTGGCCCAt > 1:301195/1‑136 (MQ=255) gCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGTGGCCCAt > 1:80145/1‑136 (MQ=255) cGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGTGGCCCATCGCAtggtg > 1:11955/1‑136 (MQ=255) tGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGTGGCCCATCGCATGGTGGGCGACCTGATAGTTGAGGCCGTc < 1:314986/136‑1 (MQ=255) tGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACATAGGCGTGCAGGTGGCCCATCGCATGGTGGGCGACCTGATAGTTGAGGCCGTCGAGCAg < 1:223692/136‑1 (MQ=255) tCGTACAGCGGCCGCGACAGCGATGGCAGGTCGCATTCCACGCGGTACTGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGTGGCCCATCGCATGGTGGGCGACCTGATAGTTGAGGCCGTCGAGCAGGACCa < 2:365603/136‑1 (MQ=255) tCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGTGGCCCATCGCATGGTGGGCGACCTGATAGTTGAGGCCGTCGAGCAGGACCa < 2:62186/136‑1 (MQ=255) ccGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGTGGCCCATCGCATGGTGGGCGACCTGATAGTTGAGGCCGTCGAGCAGGACCAGGATGACGTtg > 2:192825/1‑136 (MQ=255) gCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGTGGCCCATCGCATGGTGGGCGACCTGATAGTTGAGGCCGTCGAGCAGGACCAGGATGACGTTGTGTTGCATGTTTTCTCCAGTc > 1:334309/1‑136 (MQ=255) ggTCGGCCTCGACGTAGGCGTGCAGGTGGCCCATCGCATGGTGGGCGACCTGATAGTTGAGGCCGTCGAGCAGGACCAGGATGACGTTGTGTTGCATGTTTTCTCCAGTCATGAACCGCGTTGGCACCTTCGCggg > 1:503641/1‑136 (MQ=255) | GTTGGTTGGACAGGCGGTTGATGTTGTTGTGCACGATGCCGCTGTCGATCGGTGGCACGCCGGTGAGGATGCATTCGTACAGCGGCCGCGACAGCGATGGCAGCTCGCATTCCACGCGGTACAGCGCGGCGCGGTCGGCCTCGACGTAGGCGTGCAGGTGGCCCATCGCATGGTGGGCGACCTGATAGTTGAGGCCGTCGAGCAGGACCAGGATGACGTTGTGTTGCATGTTTTCTCCAGTCATGAACCGCGTTGGCACCTTCGCGGG > NC_002947/1930434‑1930701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |