| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,973,703 | G→T | 15.9% | A36A (GCC→GCA) | gph ← | phosphoglycolate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,973,703 | 0 | G | T | 15.9% | 74.8 / 2.3 | 25 | A36A (GCC→GCA) | gph | phosphoglycolate phosphatase |
| Reads supporting (aligned to +/- strand): ref base G (11/10); new base T (2/2); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
GTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGCTCGCAAACGCATGCTTATTCCTCGCGCAGGGTCTGG > NC_002947/1973568‑1973834 | gTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAAcg > 1:405861/1‑136 (MQ=255) cGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTc < 1:87058/136‑1 (MQ=255) gAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCg > 2:355471/1‑136 (MQ=255) aaCTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGa > 1:137550/1‑136 (MQ=255) ccTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACTGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAAt > 1:310800/1‑136 (MQ=255) ccTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACTGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAAt > 1:460136/1‑136 (MQ=255) ccTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAAt > 2:23525/1‑136 (MQ=255) gAAGCCGTCAGCCTGCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCTCGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCg < 2:134799/136‑1 (MQ=255) tCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAga > 1:319732/1‑135 (MQ=255) aGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTcc > 1:520274/1‑136 (MQ=255) cTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGgc < 1:152831/136‑1 (MQ=255) ccGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGgcgc < 2:520274/136‑1 (MQ=255) gTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCgtg > 1:91828/1‑136 (MQ=255) cGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCaa > 2:529587/1‑136 (MQ=255) gCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGc < 1:355471/136‑1 (MQ=255) gcgGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTc < 1:294600/129‑1 (MQ=255) gcgGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTc > 2:294600/1‑129 (MQ=255) gcgGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGt > 1:142746/1‑136 (MQ=255) cgcCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACTGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGc < 2:310800/136‑1 (MQ=255) cgcCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACTGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGc < 2:460136/136‑1 (MQ=255) ccGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGCTCGCaa < 2:137550/136‑1 (MQ=255) ccGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGCTCGCaa < 1:243489/136‑1 (MQ=255) gagaTCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGCTCGCAAAc < 2:91828/136‑1 (MQ=255) ctcaaCGGTTCAGGTTGTCGTCAAAGGCCTGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCCTGTCGAAGAGTACTGCTCGCAAACGCATGCt < 2:319732/132‑1 (MQ=255) cAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGCTCGCAAACGCATGCTTATTCCTCGCGCAGGGTCTgg > 1:419355/1‑136 (MQ=255) cAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGCTCGCAAACGCATGCTTATTCCTCGCGCAGGGTCTgg > 1:289168/1‑136 (MQ=255) | GTCGCGCTGGTAACGCTCGAGGAACTCCAGGCGCAGGGCCTCGAAGCCGTCAGCCTCCGGGTCCATGGCGAACGCAGTGGCAACCATGGCGCGGGCGCCACCCGAGATCACGCCACGGATCAGGTTGTCGTCAACGGCCGGCAGCCCGCGTTCGGCGAGCATGGCCTGGCAAATGGCGATGAAGTCCGGCGCCGTGTCCAGCAAGGTGCCGTCCATGTCGAAGAGTACTGCTCGCAAACGCATGCTTATTCCTCGCGCAGGGTCTGG > NC_002947/1973568‑1973834 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |