| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,979,132 | C→T | 20.0% | I50I (ATC→ATT) | serC → | 3‑phosphoserine/phosphohydroxythreonine aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,979,132 | 0 | C | T | 20.0% | 40.1 / 5.0 | 20 | I50I (ATC→ATT) | serC | 3‑phosphoserine/phosphohydroxythreonine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (6/10); new base T (2/2); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
TTAACTTCTGCGCAGGCCCTGCCGCGCTTCCTGACGCTGTCCTGCAGCGCGCACAGGCCGAGATGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACTACAAGGTGCTGTTCCTGCAAGGCGGCGCCAGCCAGCAGTTCGCTGAAATCCCGCTGAACCTGCTGCCGG > NC_002947/1978999‑1979253 | ttAACTTCTGCGCAGGCCCTGCCGCGCTTCCTGACGCTGTCCTGCAGCGCGCACAGGCCGAGATGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGc > 1:336368/1‑136 (MQ=255) cgcAGGCCCTGCCGCGCTTCCTGACGCTGTCCTGCAGCGCGCACAGGCCGAGATGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGcc > 1:19273/1‑136 (MQ=255) gCCCTGCCGCGCTTCCTGACGCTGTCCTGCAGCGCGCACAGGCCGAGATGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCa < 2:336368/136‑1 (MQ=255) gcgcTTCCTGACGCTGTCCAGCAGCGCGCACAGGCCGAGATGCAGGACAGGCGTGGCAAGGGGTTGTCGGTGATGGAAAATAGCCAACCCAGCGACGATTACCTGGCCAATGCCCAAAACGCCCAGCACGACCAGc > 2:265336/1‑136 (MQ=255) gcgcTTCCTGACCCTGTACTGCAGCGCGCACAGGCCCAGAAGCTGGACCGGCGTGGCAAGGGGTTGTCGGTGATGGAAAAGATCCAACCCAGCGACGATTAACTGGCCAATGACCAAAAGGCCGAGCAGGACCAGc > 2:262750/1‑136 (MQ=255) gACGCTGTCCTGCAGCGCGCACAGGCCGAGATGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACctgc > 1:210303/1‑136 (MQ=255) tGTCCTGCAGCGCGCACAGGCCGAGATGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTgtcc < 2:527153/136‑1 (MQ=255) gTCCTGCAGCGCGCACAGGCCGAGATGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTgtccg > 2:381030/1‑136 (MQ=255) gcACAGGCCGAGATGCTGGACGGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATTGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACt < 1:265336/136‑1 (MQ=255) gcACAGGCCGAGATGCTGGACGGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATTGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACt < 1:262750/136‑1 (MQ=255) gCCGAGATGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACTACAAgg < 2:210303/136‑1 (MQ=255) gagaTGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACTACAAGGTGc < 1:419162/136‑1 (MQ=255) gagaTGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACTACAAGGTGc < 1:456450/136‑1 (MQ=255) gagaTGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACTACAAGGTGc < 1:2648/136‑1 (MQ=255) ggTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACTACAAGGTGCTGTTCCTGCAAggcg < 2:128882/135‑1 (MQ=255) cGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACTACAAGGTGCTGTTCCTGCAAggcg < 2:484948/136‑1 (MQ=255) ttGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACTACAAGGTGCTGTTCCTGCAAGGCGGCGCcagccagc > 2:10705/1‑136 (MQ=255) cATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACTACAAGGTGCTGTTCCTGCAAGGCGGCGCCAGCCAGCAGTTCGCTGAAATCCCGCTGa < 2:307198/136‑1 (MQ=255) gCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCATCCAACTACAAGGTGCTGTTCCTGCAAGGCGGCGCCAGCCAGCAGTTCGCTGAAATCCCGCTGAACctgct < 2:19273/136‑1 (MQ=255) gaTTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACTACAAGGTGCTGTTCCTGCAAGGCGGCGCCAGCCAGCAGTTCGCTGAAATCCCGCTGAACCTGCTGCCgg > 1:37594/1‑136 (MQ=255) | TTAACTTCTGCGCAGGCCCTGCCGCGCTTCCTGACGCTGTCCTGCAGCGCGCACAGGCCGAGATGCTGGACTGGCGTGGCAAGGGGTTGTCGGTGATGGAAATGAGCCATCGCAGCGACGATTACGTGGCCATCGCCGAAAAGGCCGAGCAGGACCTGCGTGACCTGCTGTCCGTCCCCTCCAACTACAAGGTGCTGTTCCTGCAAGGCGGCGCCAGCCAGCAGTTCGCTGAAATCCCGCTGAACCTGCTGCCGG > NC_002947/1978999‑1979253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |