| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,244,269 | G→T | 16.0% | Q154K (CAG→AAG) | PP_1976 ← | HlyD family secretion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,244,269 | 0 | G | T | 16.0% | 77.2 / 2.1 | 25 | Q154K (CAG→AAG) | PP_1976 | HlyD family secretion protein |
| Reads supporting (aligned to +/- strand): ref base G (13/8); new base T (2/2); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
CGGCGACATGGCTGTCCGCCGACAGGGTAGTGACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCTTTGCGCTTGTGCGTACTCGGCTTCGCGGGTGGCCAGGGCGGCACGGGCGCGTTCGACCGCCAGCTCAAAGTCGGCGGCTTCCAG > NC_002947/2244134‑2244392 | cGGCGACATGGCTGTCCGCCGACAGGGTAGTGACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTg > 1:245603/1‑136 (MQ=255) cGACATGGCTGTCCGCCGACAGGGTAGTGACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGcc < 1:91953/136‑1 (MQ=255) aCATGGCTGTCCGCCGACAGGGTAGTGACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCgg < 2:407471/136‑1 (MQ=255) cATGGCTGTCCGCCGACAGGGTAGTGACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTTGCCGgc > 2:53829/1‑136 (MQ=255) cATGGCTGTCCGCCGACAGGGTAGTGACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTTGCCGgc > 2:1953/1‑136 (MQ=255) gTCCGCCGACAGGGTAGTGACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATc < 1:265314/136‑1 (MQ=255) cAGGGTAGTGACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGcc > 2:196673/1‑117 (MQ=255) cAGGGTAGTGACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGcc < 1:196673/117‑1 (MQ=255) tAGTGACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCttgttg > 1:249357/1‑136 (MQ=255) gACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTTGCCGGCAGCAATCAGGCTGCCTTGTTGGGTg < 1:1953/136‑1 (MQ=255) gACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTTGCCGGCAGCAATCAGGCTGCCTTGTTGGGTg < 1:53829/136‑1 (MQ=255) gACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTg < 2:36727/136‑1 (MQ=255) gCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAggcg < 1:302774/136‑1 (MQ=255) ctctTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAggcgg > 1:18434/1‑136 (MQ=255) tctTCAGAGCCAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGAGAACGGTCGCACGTGGCCTGGGTCGCCGCCAACTGCGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAggcggc > 2:249848/1‑136 (MQ=255) ttCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCtt > 1:522355/1‑136 (MQ=255) gaCAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCTTTGCGCt < 2:421927/136‑1 (MQ=255) ccGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCTTTGCGCTTGTGCGTa < 2:249357/136‑1 (MQ=255) cACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCTTTGCGCTTGTGCGTACTCGGCTTCGCGGGTGGCCAg > 2:192770/1‑136 (MQ=255) cACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCTTTGCGCTTGTGCGTACTCGGCTTCGCGGGTGGCCAg > 2:460287/1‑136 (MQ=255) tCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCTTTGCGCTTGTGCGTACTCGGCTTCGCGGGTGGCCAGGGCGGCACggg > 2:392968/1‑136 (MQ=255) aaCGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGGTTTCGGTTTGTGCGTACTCGGATTCGCGGGTGCCCAGGGCGGAACGGGCGCGTTCGACCGCCAGca > 2:416759/1‑135 (MQ=255) gggTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCTTTGCGCTTGTGCGTACTCGGCTTCGCGGGTGGCCAGGGCGGCACGGGCGCGTTCGACCGCCAGCTCAAAGTcggc > 1:440775/1‑136 (MQ=255) gggTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCTTTGCGCTTGTGCGTACTCGGCTTCGCGGGTGGCCAGGGCGGCACGGGCGCGTTCGACCGCCAGCTCAAAGTcggc > 1:242512/1‑136 (MQ=255) gggTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCTTTGCGCTTGTGCGTACTCGGCTTCGCGGGTGGCCAGGGCGGCACGGGCGCGTTCGACCGCCAGCTCAAAGTcggc > 1:264831/1‑136 (MQ=255) ccAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCTTTGCGCTTGTGCGTACTCGGCTTCGCGGGTGGCCAGGGCGGCACGGGCGCGTTCGACCGCCAGCTCAAAGTCGGCGGCTTCCAg > 1:165528/1‑136 (MQ=255) | CGGCGACATGGCTGTCCGCCGACAGGGTAGTGACCCGCTCTTCAGAGACAAAACCCGGCTTGCGCAGCTTCTCGGCACGGCTCAGGTCCAGCCGTGAACGGTCGAACGTGGCCTGGGTCGCCGCCAACTGGGCCTGGCCGGCAGCAATCAGGCTGCCTTGTTGGGTGAGGCGGCTTTGCGCTTGTGCGTACTCGGCTTCGCGGGTGGCCAGGGCGGCACGGGCGCGTTCGACCGCCAGCTCAAAGTCGGCGGCTTCCAG > NC_002947/2244134‑2244392 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |