| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,280,496 | C→A | 18.4% | intergenic (‑15/+174) | PP_2005 ← / ← PP_2006 | hypothetical protein/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,280,496 | 0 | C | A | 18.4% | 57.4 / 2.5 | 22 | intergenic (‑15/+174) | PP_2005/PP_2006 | hypothetical protein/membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (5/12); new base A (2/2); total (8/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01 | |||||||||||
GCGCAGGTCGGACAGGTAGTGGCCTTCCGGGCGCTGTTGGGTCCAGAAGCCATAGCCGCAGAGGGCGGCGATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGCATGGATCCATGCACAATCGACTTTTGATCATACCAAAGGAATGGCGATGGGCGTGC > NC_002947/2280371‑2280628 | gcgcAGGTCGGACAGGTAGTTTGCTTCCGGGCGCTGTTGGGTCCAGAAGGCATTGCCGCAGAGGGCGGCGATCATCTCCTGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAagtg < 2:5557/136‑1 (MQ=255)gcgcAGGTCGGACAGGTAGTGGCCTTCCGGGCGCTGTTGGGTCCAGAAGCCATAGCCGCAGAGGGCGGCGATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAagtg < 2:135664/136‑1 (MQ=255)gcgcAGGTCGGACAGGTAGTGGCCTTCCGGGCGCTGTTGGGTCCAGAAGCCATAGCCGCAGAGGGCGGCGATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAagtg < 2:343047/136‑1 (MQ=255)gcgcAGGTCGGACAGGTAGTGGCCTTCCGGGCGCTGTTGGGTCCAGAAGCCATAGCCGCAGAGGGCGGCGATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAagtg < 2:115248/136‑1 (MQ=255)gcgcAGGTCGGACAGGTAGTGGCCTTCCGGGCGCTGTTGGGTCCAGAAGCCATAGCCGCAGAGGGCGGCGATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAagtg < 2:114898/136‑1 (MQ=255) ttGGGTCCAGAAGCCATAGCCGCAGAGGGCGGCGATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTcgc < 1:404680/136‑1 (MQ=255) tGGGTCCAGAAGCCATAGCCGCAGAGGGCGGCGATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTcgcg > 2:337425/1‑136 (MQ=255) ccAGAAGCCATAGCCGCAGAGGGCGGCGATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTtg < 1:1278/136‑1 (MQ=255) gCCGCAGAGGGCGGCGATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGa < 1:80384/136‑1 (MQ=255) gCAGAGGGCGGCGATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAAGTGGAGGGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGagca < 1:238410/136‑1 (MQ=255) ggcgATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGc > 2:451924/1‑136 (MQ=255) ggcgATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGc > 2:397621/1‑136 (MQ=255) gATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGCATg < 2:551863/136‑1 (MQ=255) gATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTAGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGCATg > 2:189496/1‑136 (MQ=255) gATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTAGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGCATg > 2:186979/1‑136 (MQ=255) cgcCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGCATGGATCCATGcaca < 1:411464/136‑1 (MQ=255) gCCAGGAGTTTTCGCATCAAGCGAGGGTATTGGCTAAAAGAGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCATGTGATCGGTTTGAGCAGCGCACTGCATGGATCCAAGCACAATCGACtaatgaa > 1:222471/1‑130 (MQ=255) tttCGCATCAAGCGTTGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGCATGGATCCATGCACAATCGACTTTTGATCATACCaaa < 1:3892/136‑1 (MQ=255) aGCGTGGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGCATGGATCCATGCACAATCGACTTTTGATCATACCAAAGGAATGGCGa > 2:347211/1‑136 (MQ=255) tGGGTATTAGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGCATGGATCCATGCACAATCGACTTTTGATCATACCAAAGGAATGGCGATggg < 1:189496/136‑1 (MQ=255) tGGGTATTAGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGCATGGATCCATGCACAATCGACTTTTGATCATACCAAAGGAATGGCGATggg < 1:186979/136‑1 (MQ=255) aTTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGCATGGATCCATGCACAATCGACTTTTGATCATACCAAAGGAATGGCGATGGGCGTGc > 2:512893/1‑136 (MQ=255) | GCGCAGGTCGGACAGGTAGTGGCCTTCCGGGCGCTGTTGGGTCCAGAAGCCATAGCCGCAGAGGGCGGCGATCATCACCAGCGCCAGGGCGCTTGCCAGGAGTTTTCGCATCAAGCGTGGGTATTCGCTAAAAGTGGAGTGGCCATACGGAGCGATGCAGCCGCAATCTCGCGCCTTGTGATCGGTTTGAGCAGCGCACTGCATGGATCCATGCACAATCGACTTTTGATCATACCAAAGGAATGGCGATGGGCGTGC > NC_002947/2280371‑2280628 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |