| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,393,254 | G→A | 14.8% | *341* (TGA→TAA) | pyrD → | dihydroorotate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,393,254 | 0 | G | A | 14.8% | 63.7 / 4.3 | 27 | *341* (TGA→TAA) | pyrD | dihydroorotate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (11/12); new base A (2/2); total (13/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.38e-01 | |||||||||||
CATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCAT‑AAAAAAGGGCCCCTTCAAGGGGCCCCTGGGCCTTCGCCCGCCGCCCGGATGGGGCGTGCATGGTAAATCGA > NC_002947/2393120‑2393383 | cATTACCGAAGGCCGCCATGCGGCCGAGAAGAGCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGa < 2:417062/136‑1 (MQ=255)cATTACCGAAGCCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGa < 2:544539/136‑1 (MQ=255) cGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAgcc < 2:272276/136‑1 (MQ=255) cGAAGGCCGCCATGCGGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAgcc < 2:31911/136‑1 (MQ=255) acggCCCCCTTGCGGCCTAGAAGAGCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAgcccg < 1:71415/134‑1 (MQ=255) gccgccATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAgcccgccc > 1:490413/1‑136 (MQ=255) ccgccATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGcccc < 2:490413/136‑1 (MQ=255) cgccATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGccccg > 2:497004/1‑135 (MQ=255) tgctgcCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTAGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTggg > 1:150262/1‑136 (MQ=255) cGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCAACACCGGGCCGGCGCTGATTCGCGACGCGGTGTATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGCg < 2:472149/136‑1 (MQ=255) gagaAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGCgg < 1:492631/136‑1 (MQ=255) gcgGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCa > 2:56775/1‑136 (MQ=255) ggCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGGGGGTGGGTGCAGc > 1:35411/1‑136 (MQ=255) ggCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGtgctgc > 2:422832/1‑136 (MQ=255) gggCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCAGCtgatg < 2:375337/136‑1 (MQ=255) cTCGGGCTTCATGACCCTCGGGCCGGCGCTTTTTCGCGTTGCGGTGGATGCTCTCGCGGCAATGCCGCGGTGACCAGCCCGCCCCGGTGGGGGGGCGGGTGGGTGAAGCTGATGAGGTTTGCCAAGGCTT‑aaaaac < 2:497225/136‑2 (MQ=255) tCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTAACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCATAAAAAAAGGGcccc > 1:91721/1‑136 (MQ=255) tCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTAACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCATAAAAAAAGGGcccc > 1:89295/1‑136 (MQ=255) tCTACAATGGGCCGGCGCTGTTTGGCGAAGCGGTGTATGCTATCGCGGCATTGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCAT‑AAAAAAGGGCCCCTTCa < 2:35411/136‑1 (MQ=255) ggCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCAT‑AAAAAAGGGCCCCTTCAAGGGGCCCCTggg > 1:244346/1‑136 (MQ=255) gcgcTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCAT‑AAAAAAGGGCCCCTTCAAGGGGCCCCTGGGc > 1:285288/1‑136 (MQ=255) cgcTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGGGCAGCTGATGAGGTTTGCCAAGGCAT‑AAAAAAGGGCCCCTTCAAGGGGCCCCTGGGcc > 1:149330/1‑136 (MQ=255) gATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTAACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCATAAAAAAAGGGCCCCTTCAAGGGGCCCCTGGGCCTTc < 2:89295/136‑1 (MQ=255) gATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTAACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCATAAAAAAAGGGCCCCTTCAAGGGGCCCCTGGGCCTTc < 2:91721/136‑1 (MQ=255) cgATGCGGTGGATGCTAGCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGGGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCAT‑AAAAAAGGGCCCCTTCAAGGGGCCCCTGGGCCTTcgcccgcc < 2:451305/136‑1 (MQ=255) cgcgGTGACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCAT‑AAAAAAGGGCCCCTTCAAGGGGCCCCTGGGCCTTCGCCCGCCGCCCGGATGGGGCGTGCATGGTAAATCGa > 1:14838/1‑136 (MQ=255) cgcgGTGACCAGCCCGCCCCGGTGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCAT‑AAAAAAGGGCCCCTTCAAGGGGCCCCTGGGCCGTCGCCCGCCGCCCGGATGGGGCGTGCATGGTAAATCgg > 1:397161/1‑135 (MQ=255) | CATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCTGTGGGGGGGCGGGTGGGTGCAGCTGATGAGGTTTGCCAAGGCAT‑AAAAAAGGGCCCCTTCAAGGGGCCCCTGGGCCTTCGCCCGCCGCCCGGATGGGGCGTGCATGGTAAATCGA > NC_002947/2393120‑2393383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |