| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,552,855 | C→A | 21.0% | A113A (GCG→GCT) | rhtA ← | threonine/homoserine exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,552,855 | 0 | C | A | 21.0% | 49.8 / 2.2 | 19 | A113A (GCG→GCT) | rhtA | threonine/homoserine exporter |
| Reads supporting (aligned to +/- strand): ref base C (9/6); new base A (2/2); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATAGGCGGCGCCCACTGGGTCGAGTGCCTGGCCGCTGTGACCTGCCGGCAGCAACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAGGGCCATATAGAAGAGGAAGTTCATGCCGCCCAGTGCCATGCCGTAGATGACCACATTGC > NC_002947/2552733‑2552976 | gcATAGGCGGCGCCCACTGGGTCGAGTGCCTGGCCGCTGTGACCTGCCGGCAGCAACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACg > 2:265715/1‑136 (MQ=255) gcggcgCCCACTGGGTCGAGTGCCTGGCCGCTGTGACCTGCCGGCAGCAACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCgg > 1:477418/1‑136 (MQ=255) cgcCCACTGGGTCGAGTGCCTGGCCGCTGTGACCTGCCGGCAGCAACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGaa < 2:213678/136‑1 (MQ=255) cccACTGGGTCGAGTGCCTGGCCGCTGTGACCTGCCGGCAGCAACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAAtt > 2:306435/1‑136 (MQ=255) ccGCTGTGACCTGCCGGCAGCAACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGa > 1:265109/1‑136 (MQ=255) gCTGTGACCTGCCGGCAGCAACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGAtt < 1:265715/136‑1 (MQ=255) gCCGGCAGCAACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGttt < 1:256556/136‑1 (MQ=255) gCCGGCAGCAACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGttt < 2:59183/136‑1 (MQ=255) gcagcaACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATAGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAg > 2:348096/1‑136 (MQ=255) gcagcaACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATAGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAg > 2:350938/1‑136 (MQ=255) aaCAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAGGGCCa > 1:520494/1‑136 (MQ=255) cTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAGGGCCATATAGAAGAGGaa > 2:56637/1‑136 (MQ=255) cGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAGGGCCATATAGAAGAGGAAGtt > 1:513265/1‑136 (MQ=255) tGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAGGGCCATATAGAAGAGGAAGTTCAt > 1:429767/1‑136 (MQ=255) aaTCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAGGGCCATATAGAAGAGGAAGTTCATGCCGCCCAGt > 1:149377/1‑136 (MQ=255) ggAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAGGGCCATATAGAAGAGGAAGTTCATGCCGCCCAGTGCCATGcc < 2:265109/136‑1 (MQ=255) cgcgAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAGGGCCATATAGAAGAGGAAGTTCATGCCGCCCAGTGCCATGCCGTAGATGACCACATTgc < 2:403973/136‑1 (MQ=255) cgcgAGGAGAAGATAGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAGGGCCATATAGAAGAGGAAGTTCATGCCGCGCAGTGCCATGCCGTAGATGACCACATTgc < 1:350938/136‑1 (MQ=255) cgcgAGGAGAAGATAGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAGGGCCATATAGAAGAGGAAGTTCATGCCGCGCAGTGCCATGCCGTAGATGACCACATTgc < 1:348096/136‑1 (MQ=255) | GCATAGGCGGCGCCCACTGGGTCGAGTGCCTGGCCGCTGTGACCTGCCGGCAGCAACAGCAGCAGGCCTGCGATGGCCAGGGCAATCCACAGGAAATCGATCGCTCGGCGCGAGGAGAAGATCGCCACGGCCAACGGGCCGGTGAATTCCAGGGCGACGGCGATACCGATTGGTACCGTTTGCAGGGCCATATAGAAGAGGAAGTTCATGCCGCCCAGTGCCATGCCGTAGATGACCACATTGC > NC_002947/2552733‑2552976 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |