| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,601,505 | G→T | 26.7% | G411V (GGG→GTG) | PP_2273 → | DNA polymerase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,601,505 | 0 | G | T | 26.7% | 29.3 / 3.1 | 15 | G411V (GGG→GTG) | PP_2273 | DNA polymerase I |
| Reads supporting (aligned to +/- strand): ref base G (5/6); new base T (2/2); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
GGCTTCTGGTCGGTGCCGATGCCTCAGGCCTGGAGCTGCGCTGCCTGGCTCACTTCATGGCTCGCTACGATGGTGGCAAGTACGTAGACATTCTCCTCAACGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGCACAAGCTGTACCGCGATGGTGCGAAGACCTTTATCTACGCCTTCCTCTATGG > NC_002947/2601403‑2601628 | ggctTCTGGTCGGTGCCGATGCCTCAGGCCTGGAGCTGCGCTGCCTGGCTCACTTCATGGCTCGCTACGATGGTGGCAAGTACGTAGACATTCTCCTCAACGGGGACATCCACTGGGCCAACGTCCAGGCCATggg < 2:186709/136‑1 (MQ=255) ctgcgctgcCTGGCTCACTTCATGGCTCGCTACGATGGTGGCAAGTACGTAGACATTCTCCTCAACGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGc > 1:301140/1‑136 (MQ=255) gcgctgcCTGGCTCACTTCATGGCTCGCTACGATGGTGGCAAGTACGTAGACATTCTCCTCAACGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTg < 2:301140/136‑1 (MQ=255) tgcCTGGCTCACTTCATGGCTCGCTACGATGGTGGCAAGTACGTAGACATTCTCCTCAACGTGGGCATCCACTGGGCCAACGTCCAGGCCATTGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGcaca > 1:440533/1‑136 (MQ=255) tgcCTGGCTCACTTCATGGCTCGCTACGATGGTGGCAAGTACGTAGACATTCTCCTCAACGTGGACATCCACTGGGCCAACGTCCAGGCCATTGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGcaca > 1:393602/1‑136 (MQ=255) cTCGCTACGATGGTGGCAAGTACGTAGACATTCTCCTCAACGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGCACAAGCTGTACCGCGATGGTGc < 1:507964/136‑1 (MQ=255) aGTACGTAGACATTCTCCTCAACGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGCACAAGCTGTACCGCGATGGTGCGAAGACCTTTATCTACGc > 2:263170/1‑136 (MQ=255) ttCTCCTCAACGTGGGCATCCACTGGGCCAACGTCCAGGCCATTGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGCACAAGCTGTACCGCGATGGTGCGAAGACCTTTATCTACGCCTTCCTCTATgg < 2:440533/136‑1 (MQ=255) ttCTCCTCAACGTGGACATCCACTGGGCCAACGTCCAGGCCATTGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGCACAAGCTGTACCGCGATGGTGCGAAGACCTTTATCTACGCCTTCCTCTATgg < 2:393602/136‑1 (MQ=255) cctcAACGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGcac > 1:345128/1‑82 (MQ=255) cctcAACGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGcac > 1:411233/1‑82 (MQ=255) cctcAACGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGcac < 2:345128/82‑1 (MQ=255) cctcAACGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGcac < 2:411233/82‑1 (MQ=255) cGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGc < 1:534616/74‑1 (MQ=255) cGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGc > 2:534616/1‑74 (MQ=255) | GGCTTCTGGTCGGTGCCGATGCCTCAGGCCTGGAGCTGCGCTGCCTGGCTCACTTCATGGCTCGCTACGATGGTGGCAAGTACGTAGACATTCTCCTCAACGGGGACATCCACTGGGCCAACGTCCAGGCCATGGGCATCACCAGTGAGAAGCGTGACGACCACAACACGCTGCACAAGCTGTACCGCGATGGTGCGAAGACCTTTATCTACGCCTTCCTCTATGG > NC_002947/2601403‑2601628 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |