| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,671,564 | G→T | 17.4% | R812L (CGG→CTG) | acnA‑II → | aconitate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,671,564 | 0 | G | T | 17.4% | 62.9 / 2.6 | 23 | R812L (CGG→CTG) | acnA‑II | aconitate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (9/10); new base T (2/2); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
GCTTCGAGCGTATTCACCGCACCAACCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGTTACCCGTGCCAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGA > NC_002947/2671429‑2671696 | gCTTCGAGCGTATTCACCGCACCAACCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACg < 2:113878/136‑1 (MQ=255)gCTTCGAGCGTATTCACCGCACCAACCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGAATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACg < 2:312645/136‑1 (MQ=255) tgCGTATTCACCGCACCAACCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCgac < 1:423299/135‑1 (MQ=255) aTTCACCGCACCAACCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCgaccctga < 1:200650/136‑1 (MQ=255) tCACCGCACCAACCTGGTAGGCTTGGGAGTGCTGCCGCTGGAGTTCTAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGTTGGGAGCGAAACCTTTTACGCTCTGGGTGCGCGTACGCCTCGGGCgaccctgacc < 2:703/136‑1 (MQ=255) cACCGCACCAACCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCgaccctgaccc > 1:154367/1‑136 (MQ=255) cAACCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGtt < 2:415132/136‑1 (MQ=255) cAACCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGtt < 1:411333/136‑1 (MQ=255) cAACCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGtt < 2:332920/136‑1 (MQ=255) cAACCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGtt > 2:18579/1‑136 (MQ=255) aaCCTGGTAGGCATTGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACTGGCGACCCTGACCCTGGTGGtt > 1:40504/1‑135 (MQ=255) aaCCTGGTAGGCATTGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACTGGCGACCCTGACCCTGGTGGtt < 2:40504/135‑1 (MQ=255) aaCCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACTGGCGACCCTGACCCTGGTGGtt > 1:259409/1‑135 (MQ=255) aaCCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACTGGCGACCCTGACCCTGGTGGtt < 2:259409/135‑1 (MQ=255) ggCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGTTACCCGTGCCa > 1:108303/1‑136 (MQ=255) gcCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGTTACCCGTGCCAATGGCGAGTGCCTg > 1:39388/1‑136 (MQ=255) gcCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGTTACCCGTGCCAATGGCGAGTGCCTg < 2:536478/136‑1 (MQ=255) gCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGTTACCCGTGCCAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAgt > 1:540285/1‑136 (MQ=255) ggCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGTTACCCGTGCCAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCAGGACACCGCCGACGAAGTGTAGATca > 1:74572/1‑135 (MQ=255) aaCCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGTTACCCGTGCCAATGGCGAGTGCCTGGAAGTACCGGTGa > 2:339290/1‑92 (MQ=255) aaCCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGTTACCCGTGCCAATGGCGAGTGCCTGGAAGTACCGGTGa < 1:339290/92‑1 (MQ=255) cGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGTTACCCGTGCCAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGtg < 1:51737/136‑1 (MQ=255) gTACGCCACGGGCGACCCTGACCCTGGTGGTTACCCGTGCCAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGc > 2:217152/1‑136 (MQ=255) cGCCACGGGCGACCCTGACCCTGGTGGTTACCCGGGCCAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCa < 1:18579/136‑1 (MQ=255) cACGGGCGCCCCTGACCCTGGTGGTTACCCGTGCCAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGATGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAggg > 1:502885/1‑135 (MQ=255) | GCTTCGAGCGTATTCACCGCACCAACCTGGTAGGCATGGGCGTGCTGCCGCTGGAGTTCAAGCCGGGCACCGACCGCAAGACCCTGGGCCTGGATGGCAGCGAAACCTATGACGTGCTGGGTGCGCGTACGCCACGGGCGACCCTGACCCTGGTGGTTACCCGTGCCAATGGCGAGTGCCTGGAAGTACCGGTGACCTGCCGTCTGGACACCGCCGAGGAAGTGTCGATCTACGAAGCGGGCGGGGTGCTGCAGCGCTTTGCCCAGGA > NC_002947/2671429‑2671696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |