Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,702,220 C→A 16.1% G357W (GGG→TGG)  PP_2364 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,702,2200CA16.1% 92.5 / 4.4 31G357W (GGG→TGG) PP_2364hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (15/11);  new base A (2/3);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

CGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCTCGTCACGGGTATAGCC  >  NC_002947/2702093‑2702355
                                                                                                                               |                                                                                                                                       
cgccTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGCCCACCCGGTGCCgg                                                                                                                                 >  1:493205/1‑136 (MQ=255)
cgccTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACgg                                                                                                                                 >  1:344913/1‑136 (MQ=255)
  ccTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGCCGGCa                                                                                                                               >  2:384608/1‑136 (MQ=255)
      gcgcgACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGGCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCgg                                                                                                                           <  1:225492/135‑1 (MQ=255)
         cgACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCTACCACCCGGTGACGGCATCGGgcg                                                                                                                        >  1:509973/1‑136 (MQ=255)
                       cGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCAGGTGACGGCATCGGGCGCGTCGTCATGGTcc                                                                                                          >  2:118017/1‑136 (MQ=255)
                       cGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCAGGTGACGGCATCGGGCGCGTCGTCATGGTcc                                                                                                          >  2:94775/1‑136 (MQ=255)
                              gCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACg                                                                                                   >  2:532348/1‑136 (MQ=255)
                                          gAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCg                                                                                       >  1:355702/1‑136 (MQ=255)
                                                  gcgcAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGAc                                                                               <  1:532348/136‑1 (MQ=255)
                                                        aTCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAg                                                                         <  2:548437/136‑1 (MQ=255)
                                                         tCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGt                                                                        <  1:15301/136‑1 (MQ=255)
                                                           cccAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAg                                                                         <  2:64223/133‑1 (MQ=255)
                                                           cccAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAg                                                                         >  1:64223/1‑133 (MQ=255)
                                                                       ggCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGccc                                                          >  1:30663/1‑136 (MQ=255)
                                                                       ggCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGccc                                                          >  2:97041/1‑136 (MQ=255)
                                                                       ggCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGccc                                                          >  2:489578/1‑136 (MQ=255)
                                                                                            cccGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGcgtc                                     >  1:117206/1‑136 (MQ=255)
                                                                                              cGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGcgtcgt                                                                                                                  >  2:62449/1‑57 (MQ=255)
                                                                                              cGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGcgtcgt                                                                                                                  <  1:62449/57‑1 (MQ=255)
                                                                                              cGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGcgtcgt                                   >  2:522467/1‑136 (MQ=255)
                                                                                                               cgcgcCCCCGACCACCAGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACGGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCt                  <  1:94775/136‑1 (MQ=255)
                                                                                                               cgcgcCCCCGACCACCAGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCt                  <  1:118017/136‑1 (MQ=255)
                                                                                                               cgcgcCCCCGACCACCAGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCt                  <  1:156174/136‑1 (MQ=255)
                                                                                                                  gcCCCCTACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCTCGt               <  2:509973/136‑1 (MQ=255)
                                                                                                                  gcCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCTCGt               <  2:493205/136‑1 (MQ=255)
                                                                                                                  gcCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCTCGt               <  2:344913/136‑1 (MQ=255)
                                                                                                                    ccccGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCTCGTCa             >  2:516355/1‑136 (MQ=255)
                                                                                                                       cGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCTCGTCACgg          <  1:97041/136‑1 (MQ=255)
                                                                                                                       cGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCTCGTCACgg          <  1:516355/136‑1 (MQ=255)
                                                                                                                            accCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATATCCAGCGACAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGAGGTCGCCAGTACGGGCTCGTGACGGGtatt     >  1:146872/1‑135 (MQ=255)
                                                                                                                               cGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCTCGTCACGGGTATAGcc  <  2:355702/136‑1 (MQ=255)
                                                                                                                               |                                                                                                                                       
CGCCTGTCGCGACGGGCTGCTCCCGCAGGGGCTGCGCAAGGCGAGGTTCAGCGCAAATCCCCAACCATGCTGGCCAAGGTCTCCAGCACCGCCCCGGCCAGTGCCTTGGAGCGCGCCCCCGACCACCCGGTGACGGCATCGGGCGCGTCGTCATGGTCCTTGAACGGCATTTCCAGCGTCAGTGACAGGCAGTCATGGGCCATGCCCACCGCATTGCAGGCCAGCGTCGTGTTCGCCTGTCCGGGCTCGTCACGGGTATAGCC  >  NC_002947/2702093‑2702355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑