| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,788,717 | C→A | 25.0% | R60S (CGC→AGC) | PP_2437 → | acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,788,717 | 0 | C | A | 25.0% | 32.4 / 3.2 | 16 | R60S (CGC→AGC) | PP_2437 | acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (8/4); new base A (2/2); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
GCACTGCGCGAGCGTGTCACTGCGTTCATGGACGCTCATGTCTACCCCGCCGAAGCCGTGTTCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTACGCACCGCTGGCAGAGATCATGGGCCGCTCGTTGCTCGGGCCGGAGCCGTTCAACTGCTCGGCG > NC_002947/2788585‑2788851 | gctcTGCGCGATCTTGTCACTGCGATCATGGACGCTCATGTCTACCCCGCCGAAGCGGTGTTCGAGCGCCATGTCGCCGAGGGTGACAGCTGTCAGCCCACTGCTATGATTGTGGAGCTTAAAGCCAAGGCCcgcg < 2:451097/133‑1 (MQ=255) aCTGCGCGAGCGTGTCACTGCGTTCATGGACGCTCATGTCTACCCCGCCGAAGCCGTGTTCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGAGcc > 1:341517/1‑136 (MQ=255) cTGCGCGAGCGTGTCACTGCGTTCATGGACGCTCATGTCTACCCCGCCGAAGCCGTGTTCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCg < 1:366796/136‑1 (MQ=255) tGCGCGAGCGTGTCACTGCGCTCATGGACGCTCATGTCTACCCCGCCGAAGCCGTGGGCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCGa < 2:89258/136‑1 (MQ=255) cgAGCGTGTCACTGCGTTCATGGACGCTCATGTCTACCCCGCCGAAGCCGTGTTCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCAGCGCCGAAggg > 1:415886/1‑136 (MQ=255) cgAGCGTGTCACTGCGTTCATGGACGCTCATGTCTACCCCGCCGAAGCCGTGTTCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCAGCGCCGAAggg > 1:418449/1‑136 (MQ=255) gtCACTGCGTTCATGGACGCTCATGTCTACCCCGCCGAAGCCGTGTTCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCGAAGGGTTGTGGa > 1:30169/1‑136 (MQ=255) cATGGACGCTCATGTCTACCCCGCCGAAGCCGTGTTCGAGCGCCAGGGCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCGAAGGGTTGTGGAACCTGTTCTTg < 2:103997/136‑1 (MQ=255) aTGTCTACCCCGCCGAAGCCGTGTTCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGa > 1:532125/1‑136 (MQ=255) aTGTCTACCCCGCCGAAGCCGTGTTCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGa > 1:427660/1‑136 (MQ=255) gccgAAGCCGTGTTCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTg > 2:214492/1‑136 (MQ=255) cgAAGCCGTGTTCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGc > 1:515886/1‑136 (MQ=255) gcCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCAGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTa < 2:415886/136‑1 (MQ=255) gcCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCAGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTa < 2:418449/136‑1 (MQ=255) ccAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTAc > 2:495162/1‑136 (MQ=255) cTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTACGCACCGCTGGCAGAGATCATGGGCCGCTCGtt > 2:491222/1‑136 (MQ=255) aTGGAGGAGCTTAAAGCCAAGGCCCGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTACGCACCGCTGGCAGAGATCATGGGCCGCTCGTTGCTCGGGc < 2:121888/136‑1 (MQ=255) cccGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTACGCACCGCTGGCAGAGATCATGGGCCGCTCGTTGCTCGGGCCGGAGCCGTTCAACTGCTCGgc > 1:538985/1‑136 (MQ=255) cccGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTACGCACCGCTGGCAGAGATCATGGGCCGCTCGTTGCTCGGGCCGGAGCCGTTCAACTGCTCGgc > 2:307925/1‑136 (MQ=255) cccGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTACGCACCGCTGGCAGAGATCATGGGCCGCTCGTTGCTCGGGCCGGAGCCGTTCAACTGCTCGgc > 1:287721/1‑136 (MQ=255) ccGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTACGCACCGCTGGCAGAGATCATGGGCCGCTCGTTGCTCGGGCCGGAGCCGTTCAACTGCTCGgcg > 2:115252/1‑136 (MQ=255) ccGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTACGCACCGCTGGCAGAGATCATGGGCCGCTCGTTGCTCGGGCCGGAGCCGTTCAACTGCTCGgcg > 2:186962/1‑136 (MQ=255) | GCACTGCGCGAGCGTGTCACTGCGTTCATGGACGCTCATGTCTACCCCGCCGAAGCCGTGTTCGAGCGCCAGGTCGCCGAGGGTGACCGCTGGCAGCCCACTGCAATCATGGAGGAGCTTAAAGCCAAGGCCCGCGCCGAAGGGTTGTGGAACCTGTTCTTGCCCGAGTCGGAATACGGCGCTGGCCTGAGCAACCTGGAGTACGCACCGCTGGCAGAGATCATGGGCCGCTCGTTGCTCGGGCCGGAGCCGTTCAACTGCTCGGCG > NC_002947/2788585‑2788851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |