Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	478,113	G→T	26.8%	W123L (TGG→TTG)	PP_0393 →	2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	478,113	0	G	T	26.8%	23.6 / 4.9	15	W123L (TGG→TTG)	PP_0393	2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base G (7/4);  new base T (2/2);  total (9/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

GCTATGCACCGGACCGCAAAGGGTTGCCGCTGGATATCGACGTGCTGATGTATGACGATTTGCACGGTACCTTCGATGGGCTGGTATTGCCCCGGGCCGAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCGGGTGTGGGCAAGTGCATGGCGCAGCTGTGGCAGGAAGCGCAGATCGATCAGGTACTGGCCCCCGTGGCCTTTGAGTGGCGCG  >  NC_002947/477987‑478232                                                                                                                               |                                                                                                                        gCTATGCACCGGACCGCAAAGGGTTGCCGCTGGATATCGACGTGCTGATGTATGACGATTTGCACGGTACCTTCGATGGGCTGGTATTGCCCCGGGCCGAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTc                                                                                                                >  2:30463/1‑136 (MQ=255)    aTGCACCGGACCGCAAAGGGTTGCCGCTGGATATCGACGTGCTGATGTATGACGATTTGCACGGTACCTTCGATGGGCTGGTATTGCCCCGTGCCGAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGtt                                                                                                             <  1:308680/136‑1 (MQ=255)                    agtgTTGCCGCTGGATATCGACGTGCTGATGTATGACGATTTGCACGGTACCTTCGATGGGCTGGTATTGCCCCTGGCCGAGATTCTGAAGAACGCTTTTGTGCTGTTGCCGCTGTCGTTGCTGGCGCCCGACCTg                                                                                             >  2:294353/4‑136 (MQ=255)                    agtgTTGCCGCTGGATATCGACGTGCTGATGTATGACGATTTGCACGGTACCTTCGATGGGCTGGTATTGCCCCTGGCCGAGATTCTGAAGAACGCTTTTGTGCTGTTGCCGCTGTCGTTGCTGGCGCCCGACCTg                                                                                             >  2:296407/4‑136 (MQ=255)                              cTGGATATCGACGTGCTGATGTATGACGATTTGCACGGTACCTTCGATGGGCTGGTATTGCCCCGGGCCGAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCgg                                                                                   >  2:232536/1‑136 (MQ=255)                              cTGGATATCGACGTGCTGATGTATGACGATTTGCACGGTACCTTCGATGGGCTGGTATTGCCCCGGGCCGAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCgg                                                                                   >  2:253258/1‑136 (MQ=255)                                              tGATGTATGACGATTTGCACGGTACCTTCGATGGGCTGGTATTGCCCCGGGCCGAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCGGGTGTGGGCAAGTGCAt                                                                   >  2:77590/1‑136 (MQ=255)                                              tGATGTATGACGATTTGCACGGTACCTTCGATGGGCTGGTATTGCCCCGGGCCCAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGTTGCTGGCGACCCACCTGGTGGATCCCGGTGTGGGCAAGTGCaa                                                                   >  1:413488/1‑135 (MQ=255)                                                         gATTTGCACGGTACCTTCGATGGGCTGGTATTGCCCCGGGCCGAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCGGGTGTGGGCAAGTGCATGGCGCAGCtgt                                                        <  1:77590/136‑1 (MQ=255)                                                            ttGCACGGTACCTTCGATGGGCTGGTATTGCCCCGGGCCGAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCGGGTGTGGGCAAGTGCATGGCGCAGCTGTGGc                                                     <  1:160121/136‑1 (MQ=255)                                                                                                  cGAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCGGGTGTGGGCAAGTGCATGGCGCAGCTGTGGCAGGAAGCGCAGATCGATCAGGTACTGGCCCCCGTGGcc               >  1:40419/1‑136 (MQ=255)                                                                                                  cGAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCGGGTGTGGGCAAGTGCATGGCGCAGCTGTGGCAGGAAGCGCAGATCGATCAGGTACTGGCCCCCGTGGcc               >  2:409812/1‑136 (MQ=255)                                                                                                          tGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCGGGTGTGGGCAAGTGCATGGCGCAGCTGTGGCAGGAAGCGCAGATCGATCAGGTACTGGCCCCCGTGGCCTTTGAGTg       <  2:215246/136‑1 (MQ=255)                                                                                                               aaCGCTTTTGTGCTGTTGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCGGGTGTGGGCAAGTGCATGGCGCAGCTGTGGCAGGAAGCGCAGATCGATCAGGTACTGGCCCCCGTGGCCTTTGAGTGGcgcg  <  1:294353/136‑1 (MQ=255)                                                                                                               aaCGCTTTTGTGCTGTTGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCGGGTGTGGGCAAGTGCATGGCGCAGCTGTGGCAGGAAGCGCAGATCGATCAGGTACTGGCCCCCGTGGCCTTTGAGTGGcgcg  <  1:296407/136‑1 (MQ=255)                                                                                                                               |                                                                                                                        GCTATGCACCGGACCGCAAAGGGTTGCCGCTGGATATCGACGTGCTGATGTATGACGATTTGCACGGTACCTTCGATGGGCTGGTATTGCCCCGGGCCGAGATTCTGAAGAACGCTTTTGTGCTGTGGCCGCTGTCGTTGCTGGCGCCCGACCTGGTGCATCCGGGTGTGGGCAAGTGCATGGCGCAGCTGTGGCAGGAAGCGCAGATCGATCAGGTACTGGCCCCCGTGGCCTTTGAGTGGCGCG  >  NC_002947/477987‑478232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑