| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,397,745 | A→G | 23.5% | N236S (AAT→AGT) | PP_1214 → | transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,397,745 | 0 | A | G | 23.5% | 25.1 / 6.2 | 17 | N236S (AAT→AGT) | PP_1214 | transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (5/8); new base G (2/2); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
ACGCGGAAATCGTCATGCAGCCGACCACCAGTGCCGAGCTCGACCAGGACGGTGCCGAAAAGGTGCTCAAGCTGATCGACATGCTCGAAGACCTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCCCAT > NC_002947/1397625‑1397879 | aCGCGGAAATCGTCATGCAGCCGACCACCAGTGCCGAGCTCGACCAGGACGGTGCCGAAAAGGTGCTCAAGCTGATCGACATGCTCGAAGACCTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCcgc > 1:176178/1‑135 (MQ=255)aCGCGGAAATCGTCATGCAGCCGACCACCAGTGCCGAGCTCGACCAGGACGGTGCCGAAAAGGTGCTCAAGCTGATCGACATGCTCGAAGACCTCGACGACGAGCAGAACGTCTATTCCAATGCCCAGATTTCcga > 1:5199/1‑136 (MQ=255) tCGACCAGGACGGTGCCGAAAAGGTGCTCAAGCTGATCGACATGCTCGAAGACCTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCAtt > 2:242086/1‑136 (MQ=255) cccAGGACGGTGCCGAAAAGGTGCTCAAGCTGATCGACATGCTCGAAGACCTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTtg < 1:250181/135‑1 (MQ=255) cAGGACGGTGCCGAAAAGGTGCTCAAGCTGATCGACATGCTCGAAGACCTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTtgtg > 1:200466/1‑136 (MQ=255) gTGCCGAAAAGGTGCTCAAGCTGATCGACATGCTCGAAGACCTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAActc < 2:215635/136‑1 (MQ=255) cGAAAAGGTGCTCAAGCTGATCGACATGCTCGAAGACCTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAg < 2:212225/136‑1 (MQ=255) aaaaGGTGCTCAAGCTGATCGACATGCTCGAAGACCTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCg > 2:379086/1‑136 (MQ=255) cTCAAGCTGATCGACATGCTCGAAGACCTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAg < 1:19036/136‑1 (MQ=255) cTCAAGCTGATCGACATGCTCGAAGACCTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAg < 1:168753/136‑1 (MQ=255) cTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTaa < 2:200466/136‑1 (MQ=255) cgTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTc < 1:242086/136‑1 (MQ=255) aTTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTactgca < 2:207766/136‑6 (MQ=255) ttCCAGTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCt > 1:351894/1‑123 (MQ=255) ttCCAGTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCt > 1:349965/1‑123 (MQ=255) ttCCAGTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCt < 2:349965/123‑1 (MQ=255) ttCCAGTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCt < 2:351894/123‑1 (MQ=255) aaTGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCCCAt < 2:372923/136‑1 (MQ=255) | ACGCGGAAATCGTCATGCAGCCGACCACCAGTGCCGAGCTCGACCAGGACGGTGCCGAAAAGGTGCTCAAGCTGATCGACATGCTCGAAGACCTGGACGACGTGCAGAACGTCTATTCCAATGCCCAGATTTCCGACGAGATCATGGAAAACCTCGGCTAAGGTGATTTGGCATTTTGTGCCAACTCTGAGCGTTTGATAGCGCCTGTGCCGGCCCTTTCGCGGGTAAACCCGCTCCTACAGGTATTACGCCCAT > NC_002947/1397625‑1397879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |