Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,660,814	G→T	20.8%	L316M (CTG→ATG)	PP_1449 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,660,814	0	G	T	20.8%	48.5 / 2.8	19	L316M (CTG→ATG)	PP_1449	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (9/6);  new base T (2/2);  total (11/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATGTGCTCGATTGTCTTGGCGTCCAGGGTGAGCTTCTTGCCAGCCCTTGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTG  >  NC_002947/1660721‑1660940                                                                                              |                                                                                                                               tGATGTGCTCGATTGTCTTGGCGTCCAGGGTGAGCTTCTTGCCAGCCCTTGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTgcg                                                                                      >  2:6923/1‑136 (MQ=255)               gTCTTGGCGTCCAGGGTGAGCTTCTTGCCAGCCCTTGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGc                                                                        <  2:283981/136‑1 (MQ=255)                             ggTGAGCTTCTTGCCAGCCCTTGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCg                                                          <  1:6923/136‑1 (MQ=255)                                    ttcttGCCAGCCCTTGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCATGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACAt                                                   >  2:364137/1‑136 (MQ=255)                                    ttcttGCCAGCCCTTGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCATGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACAt                                                   >  2:276056/1‑136 (MQ=255)                                     tcttGCCAGCCCTTGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATg                                                  >  2:212360/1‑136 (MQ=255)                                         gCCAGCCCTTGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAgg                                              <  1:274367/136‑1 (MQ=255)                                          ccAGCCCTTGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGc                                             <  2:324834/136‑1 (MQ=255)                                                 ttGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGc                                                                                                   <  1:230463/75‑1 (MQ=255)                                                 ttGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGc                                                                                                   >  2:230463/1‑75 (MQ=255)                                                          cccGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGAGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGAt                             <  1:246943/135‑1 (MQ=255)                                                            cgcgGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCCCTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGt                           >  2:70292/1‑136 (MQ=255)                                                                 ggCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGAcc                      >  2:109529/1‑136 (MQ=255)                                                                 ggCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACCCTCGCTGCGCAGCTCCGTCGGGCGGTCGCCACTGCCGCTGACATGCAGCCCCCCGCCCGAGCTGATGTCGAcc                      >  2:417906/1‑136 (MQ=255)                                                                              aCCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCa         >  2:147437/1‑136 (MQ=255)                                                                              aCCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCa         >  1:406233/1‑136 (MQ=255)                                                                                    tCAAGCATCATGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCt   <  1:364137/136‑1 (MQ=255)                                                                                    tCAAGCATCATGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCt   <  1:276056/136‑1 (MQ=255)                                                                                     cAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTg  >  1:235503/1‑136 (MQ=255)                                                                                              |                                                                                                                               TGATGTGCTCGATTGTCTTGGCGTCCAGGGTGAGCTTCTTGCCAGCCCTTGCTTCGATCCGCGGGGCCTGGCCGTCAACCGTTTCAAGCATCAGGTCATCGGCAGCGGTGAGTTTCAGTACGCCACGCTCGCTGCGCAGCTCGGTCGGGCGGTCGCCACTGCCGCTGACATGCAGGCCCCCGGCCGAGCTGATGTCGACCCCTTCGGTGCCCAGAATCTG  >  NC_002947/1660721‑1660940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑