Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,891,634 C→T 13.8% A437V (GCG→GTG)  rmuC → DNA recombination limiting inversions protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,891,6340CT13.8% 74.8 / 4.2 29A437V (GCG→GTG) rmuCDNA recombination limiting inversions protein
Reads supporting (aligned to +/- strand):  ref base C (12/13);  new base T (2/2);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

GAGCGTCAAGGGCAGAACGCCCGCGAAATTGCCGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAAGAGCTTGCCGGCAGACCTGCTGGAGCGGGCGCTGTCCGATGAG  >  NC_002947/1891507‑1891764
                                                                                                                               |                                                                                                                                  
gAGCGTCAAGGGCAGAACGCCCGCGAAATTGCCGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAgcgc                                                                                                                            >  1:50415/1‑136 (MQ=255)
 aGCGTCAAGGGCAGAACGCCCGCGAAATTGCCGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAgcgca                                                                                                                           >  2:112198/1‑135 (MQ=255)
   cGTCAAGGGCAGAACGCCCGCGAAATTGCCGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGca                                                                                                                         >  1:364358/1‑136 (MQ=255)
    gTCAAGGGCAGAACGCCCGCGAAATTGCCGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGcaa                                                                                                                        <  1:356934/136‑1 (MQ=255)
     tCAAGGGCAGAACGCCCGCGAAATTGCCGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGcaac                                                                                                                       <  1:183332/136‑1 (MQ=255)
            cAGAACGCCCGCGAAATTGCCGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCtgt                                                                                                                <  1:299513/136‑1 (MQ=255)
                aCGCCCGCGAAATTGCCGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCACAACAAGCTGTGCGa                                                                                                            <  1:112198/136‑1 (MQ=255)
                       cgAAATTGCCGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGt                                                                                                     >  2:14187/1‑136 (MQ=255)
                       cgAAATTGACGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGCACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCgg                                                                                                     >  1:332094/1‑135 (MQ=255)
                               ccGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCt                                                                                             <  2:364358/136‑1 (MQ=255)
                               ccGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCt                                                                                             <  2:108685/136‑1 (MQ=255)
                                       gCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGcc                                                                                     >  1:177079/1‑136 (MQ=255)
                                       gCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGGTAGcc                                                                                     >  1:174968/1‑136 (MQ=255)
                                            ctggctCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGc                                                                                <  1:204969/136‑1 (MQ=255)
                                            ctggctCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGc                                                                                <  1:177954/136‑1 (MQ=255)
                                                             tCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAgctgct                                                               >  1:38121/1‑136 (MQ=255)
                                                                     ttCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGccc                                                       <  1:151259/136‑1 (MQ=255)
                                                                        aTCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTg                                                    >  1:290760/1‑136 (MQ=255)
                                                                            aGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAg                                                >  1:346916/1‑136 (MQ=255)
                                                                                ccTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGTGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAag                                            >  1:107693/1‑136 (MQ=255)
                                                                                ccTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGTGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAag                                            >  1:109757/1‑136 (MQ=255)
                                                                                       gAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAAGAGCTTGc                                     >  2:343151/1‑136 (MQ=255)
                                                                                       gAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAAGAGCTTGc                                     >  2:92790/1‑136 (MQ=255)
                                                                                          cTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAAGAGCTTGCCgg                                  <  2:110941/136‑1 (MQ=255)
                                                                                                 gCCGCTTGCAGCAGGTGGACAAGGCTTATGTGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAAGAGCTTGCCGGCAGACct                           <  2:109757/136‑1 (MQ=255)
                                                                                                 gCCGCTTGCAGCAGGTGGACAAGGCTTATGTGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAAGAGCTTGCCGGCAGACct                           <  2:107693/136‑1 (MQ=255)
                                                                                                     cTTGCTGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTATGCCGTAGCGAGGAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAAGAGCTTGCCGGCAGACctgctg                       <  1:101938/136‑1 (MQ=255)
                                                                                                              ggTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAAGAGCTTGCCGGCAGACCTGCTGGAGCGGgcg              <  2:38297/136‑1 (MQ=255)
                                                                                                                          ttATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAAGAGCTTGCCGGCAGACCTGCTGGAGCGGGCGCTGTCCGATGAg  <  2:28527/136‑1 (MQ=255)
                                                                                                                               |                                                                                                                                  
GAGCGTCAAGGGCAGAACGCCCGCGAAATTGCCGAGCGGGCCGGCTGGCTCTACGACAAGTTCGTGCTGTTCATCCAGGACCTGGACGAACTGGGCAGCCGCTTGCAGCAGGTGGACAAGGCTTATGCGGCAGCGCGCAACAAGCTGTGCGAAGGGCGTGGCAACCTGGTTAGCCGTAGCGAGCAGCTTAAGCTGCTGGGCGCCCGTGCCAGCAAGAGCTTGCCGGCAGACCTGCTGGAGCGGGCGCTGTCCGATGAG  >  NC_002947/1891507‑1891764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑