| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,592,539 | C→A | 30.8% | I304I (ATC→ATA) | PP_2266 → | DNA‑directed RNA polymerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,592,539 | 0 | C | A | 30.8% | 21.5 / 5.5 | 13 | I304I (ATC→ATA) | PP_2266 | DNA‑directed RNA polymerase |
| Reads supporting (aligned to +/- strand): ref base C (5/4); new base A (2/2); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
TACTTGGAAGAGCTGGGCAACGTCGATATGCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATCAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGTGCGGCCTCCCACCCCGTGAAGGCATCCCCCAGCCAGTCAAACCGTTCGACATCGACACCAA > NC_002947/2592459‑2592655 | tACTTGGAAGAGCTGGGCAACGTCGATATGCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATCAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGt < 1:32441/136‑1 (MQ=255) cTTGGAAGAGCTGGGCAACGTCGATATGCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATCAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGTGc < 2:158159/136‑1 (MQ=255) gAAGAGCTGGGCAACGTCGATATGCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATCAACACGCCAATCCTGAACATCATGACCa < 1:293534/103‑1 (MQ=255) gAAGAGCTGGGCAACGTCGATATGCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATCAACACGCCAATCCTGAACATCATGACCa > 2:293534/1‑103 (MQ=255) ggCAACGTCGATATGCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATCAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCACCACCTGGTGCGGCCTCCCAcccc > 1:29513/1‑136 (MQ=255) cGATATGCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATAAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGTGCGGcc > 1:152424/1‑119 (MQ=255) cGATATGCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATAAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGTGCGGcc > 1:201682/1‑119 (MQ=255) cGATATGCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATAAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGTGCGGcc < 2:152424/119‑1 (MQ=255) cGATATGCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATAAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGTGCGGcc < 2:201682/119‑1 (MQ=255) gCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATCAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGTGCGGCCTCCCACCCCGTGAAGGCATcccc < 1:187549/136‑1 (MQ=255) tCAACGCCATCCAGGCCACCGCGTGGCAGATCAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGTGCGGCCTCCCACCCTGTGAAGGCATCCCCCAGCCAGTCAAACCGTtcga > 1:132897/1‑136 (MQ=255) tCAACGCCATCCAGGCCACCGCGTGGCAGATCAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGTGCGGCCTCCCACCCCGTGAAGGCAACCCCCACCCACTCAAACCGTtcga > 1:333618/1‑136 (MQ=255) aGGCCACCGCGTGGCAGATCAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGTGCGGCCTCCCACCCCGTGAAGGCATCCCCCAGCCAGTCAAACCGTTCGACATCGACACCaa > 2:420738/1‑136 (MQ=255) | TACTTGGAAGAGCTGGGCAACGTCGATATGCCGATGGTCTACACCGCGCTCAACGCCATCCAGGCCACCGCGTGGCAGATCAACACGCCAATCCTGAACATCATGACCACGCTGTGGGAGCAGGGCGCCACCTGGTGCGGCCTCCCACCCCGTGAAGGCATCCCCCAGCCAGTCAAACCGTTCGACATCGACACCAA > NC_002947/2592459‑2592655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |