Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,198,324	G→A	23.5%	A43A (GCC→GCT)	prr ←	gamma‑aminobutyraldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,198,324	0	G	A	23.5%	29.7 / 5.3	17	A43A (GCC→GCT)	prr	gamma‑aminobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (2/11);  new base A (2/2);  total (4/13)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CCGCCTCCAGGCGGGCGAACACCGCGGCCTGGGCGTCGATGCGGTCGGCGAGGGTGAGCAGCAGTGCCGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCAGGGAGGGGTTGTAGACGTCCACGTGGTCGCCTTCGCCGGC  >  NC_002947/3198194‑3198416                                                                                                                                   |                                                                                             ccGCCTCCAGGCGGGCGAACACCGCGGCCTGGGCGTCGATGCGGTCGGCGAGGGTGAGCAGCAGTGCCGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATg                                                                                         <  1:74253/136‑1 (MQ=255) ccGCCTCCAGGCGGGCGAACACCGCGGCCTGGGCGTCGATGCGGTCGGCGAGGGTGAGCAGCAGTGCCGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATg                                                                                         <  1:167199/136‑1 (MQ=255)                    cacCGCGGCCTGGGCGTCGATGCGGTCGGCGAGGGTGAGCAGCAGTGCCGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGcc                                                                      <  1:139091/136‑1 (MQ=255)                         cgGCCTGGGCGTCGATGCGGTCGGCGAGGGTGAGCAGCAGTGCCGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTg                                                                 <  1:203390/136‑1 (MQ=255)                         cgGCCTGGGCGTCGATGCGGTCGGCGAGGGTGAGCAGCAGTGCCGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTg                                                                 <  1:114822/136‑1 (MQ=255)                                      gATGCGGTCGGCGAGGGTGAGCAGCAGTGCCGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAgg                                                    <  2:10942/136‑1 (MQ=255)                                      gATGCGGTCGGCGAGGGTGAGCAGCAGTGCCGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAgg                                                    <  2:302576/136‑1 (MQ=255)                                                 cGAGGGTGAGCAGCAGTGCCGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCag                                         <  2:214328/136‑1 (MQ=255)                                                            tgctgTGCCGCACGGTCGTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCAGGGAGGGGTTGt                              <  1:73731/132‑1 (MQ=255)                                                             gcagTGCCGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCAGGGAGGGGTTGTa                             <  2:199068/136‑1 (MQ=255)                                                                   ccGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCAGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCAGGGAGGGGTTGTa                             <  1:64942/130‑1 (MQ=255)                                                                   ccGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCAGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCAGGGAGGGGTTGTa                             <  1:66836/130‑1 (MQ=255)                                                                   ccGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCAGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCAGGGAGGGGTTGTa                             >  2:64942/1‑130 (MQ=255)                                                                   ccGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCAGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCAGGGAGGGGTTGTa                             >  2:66836/1‑130 (MQ=255)                                                                              ttGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCagg                                        >  1:150894/1‑108 (MQ=255)                                                                              ttGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCagg                                        <  2:150894/108‑1 (MQ=255)                                                                                        tttGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCAGGGAGGGGTTGTAGACGTCCACGTGGTCGCCTTCGCCGGc  >  2:35566/1‑136 (MQ=255)                                                                                                                                   |                                                                                             CCGCCTCCAGGCGGGCGAACACCGCGGCCTGGGCGTCGATGCGGTCGGCGAGGGTGAGCAGCAGTGCCGCACGGTCCTTGGGCGGTGTTTGCGACCAGGCTTCGAAGGCCGCATCCGCGCTTTGCACTGCGGCATGGACCTGTGCATGACTGGCCTCGTGGGTCTGTACCAGGACGCGGCCCAGGGAGGGGTTGTAGACGTCCACGTGGTCGCCTTCGCCGGC  >  NC_002947/3198194‑3198416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑