| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,304,972 | G→A | 28.6% | A733T (GCC→ACC) | pvdQ → | acyl‑homoserine lactone acylase PvdQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,304,972 | 0 | G | A | 28.6% | 17.6 / 5.7 | 14 | A733T (GCC→ACC) | pvdQ | acyl‑homoserine lactone acylase PvdQ |
| Reads supporting (aligned to +/- strand): ref base G (4/6); new base A (2/2); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01 | |||||||||||
CTGGTGGTGAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGC > NC_002947/3304843‑3305089 | cTGGTGGTGAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTg > 1:70561/1‑136 (MQ=255) cAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGgcg > 2:231174/1‑136 (MQ=255) cTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTg < 2:306634/136‑1 (MQ=255) cAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAg < 2:148723/136‑1 (MQ=255) tACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGACCTTTGCGGCCAAGCa > 1:381225/1‑105 (MQ=255) tACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGACCTTTGCGGCCAAGCa > 1:382079/1‑105 (MQ=255) tACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGACCTTTGCGGCCAAGCa < 2:381225/105‑1 (MQ=255) tACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGACCTTTGCGGCCAAGCa < 2:382079/105‑1 (MQ=255) aaGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAgg < 2:102682/136‑1 (MQ=255) ccAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATc > 1:337609/1‑136 (MQ=255) cAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATcc < 1:344673/136‑1 (MQ=255) ggCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTc < 1:231174/136‑1 (MQ=255) ggCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTc < 2:337609/136‑1 (MQ=255) gCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTcagccagc > 1:147272/1‑136 (MQ=255) | CTGGTGGTGAGTGGCACCAGCTACCTGCAACTGGTCAGCTTTACCGACAAGGGGCCAGAAGCCCGTGGCCTGCTTGCGTTTTCCCAGTCGAGTGAAAAGGCTTCGGCGCATGCCAGTGACCAGACCAAGGCCTTTGCGGCCAAGCAACTGGCGCTGATTCCGTTTACCGAGGCGCAGATCAAGGCAGATCCTGAGTACCGGGAAGTGGTGATCAGTGAGCGGGACAAGGGGGCGGTAGTCAGCCAGC > NC_002947/3304843‑3305089 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |