| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,385,634 | C→T | 100% | A105V (GCT→GTT) | tnpT‑I → | cointegrate resolution protein T |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,385,634 | 0 | C | T | 88.9% | 19.3 / ‑3.2 | 9 | A105V (GCT→GTT) | tnpT‑I | cointegrate resolution protein T |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (5/3); total (6/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAAC > NC_002947/3385505‑3385744 | tACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGTTCGGCt > 2:333776/1‑136 (MQ=255) aGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGTTCGGCTGGCCGaaa > 2:236481/1‑136 (MQ=255) tGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGTTCGGCTGGCCGAAAGCCGAATCca > 2:75957/1‑136 (MQ=255) gCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGTTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACg < 2:90795/136‑1 (MQ=255) gcgcAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGTTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAgg > 1:226475/1‑136 (MQ=255) gcgcGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGTTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTAcagcac > 1:390485/1‑135 (MQ=255) aCGCGAACGGCTCGATTACCAGAACCAAGTTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGc < 1:236481/136‑1 (MQ=255) gcgAACGGCTCGATTACCAGAACCAAGTTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCa < 1:75957/136‑1 (MQ=255) gAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCacc > 1:128046/1‑134 (MQ=255) | TACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAGAGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAGCAGCGGGCTCACGGAACAGCTTCAGGCTGTTCAGCAAGCGCTACAGCAGGAACAGCAGCAACGCCAAC > NC_002947/3385505‑3385744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |