Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,450,733	C→A	100%	R45R (CGC→CGA)	gpD →	tail formation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,450,733	0	C	A	100.0%	55.0 / NA	17	R45R (CGC→CGA)	gpD	tail formation
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/8);  total (9/8)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCACGTATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGCGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGACCCGCTTGGGCGCATACACCGTCGACG  >  NC_002947/3450606‑3450833                                                                                                                                |                                                                                                     ccACGTATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGc                                                                                              >  1:174251/1‑136 (MQ=255) ccACGTATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGc                                                                                              >  1:250132/1‑136 (MQ=255) ccACGTATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGc                                                                                              >  1:60831/1‑136 (MQ=255)      tATCAAATCGTCGCGTATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTg                                                                                         >  1:103696/1‑136 (MQ=255)      tATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTg                                                                                         >  2:37437/1‑136 (MQ=255)      tATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGACTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGCCGACCGAGACCAGGCTGTTg                                                                                         >  1:16584/1‑136 (MQ=255)              cgtcgCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCg                                                                                 <  1:34661/136‑1 (MQ=255)                       tGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGt                                                                        <  1:281535/136‑1 (MQ=255)                        ggCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTa                                                                       <  1:11079/136‑1 (MQ=255)                              gACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCTAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAAcg                                                                 <  2:417413/136‑1 (MQ=255)                                                aaTGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCt                                               <  2:110522/136‑1 (MQ=255)                                                     ccGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAg                                          >  1:403583/1‑136 (MQ=255)                                                           gTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCaa                                    <  1:99384/136‑1 (MQ=255)                                                                             ggACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGAccc                          >  2:290343/1‑128 (MQ=255)                                                                             ggACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGAccc                          <  1:290343/128‑1 (MQ=255)                                                                             ggACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGACCCGCTTGGgc                  >  2:251435/1‑136 (MQ=255)                                                                                             gAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGAGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGACCCGCTTGGGCGCATACACCGTcgacg  <  1:323472/136‑1 (MQ=255)                                                                                                                                |                                                                                                     CCACGTATCAAATCGTCGCGGATGGCAGCGACATTACCGCGCTGATCAATGACCGGCTGTTGCTGCTACGCACATCGGACAAGCCCGGCATGGAGTCGGACGAGTTCGAGCTGCGCATTGACGACCGCGACCAGGCTGTTGCGCTGCCGGCACGCGGTAGCAACGTCGTGGTCATGATGGGCTACGAGGGCCAAGGGCTGACCCGCTTGGGCGCATACACCGTCGACG  >  NC_002947/3450606‑3450833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑