| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,664,263 | G→T | 28.4% | K40N (AAG→AAT) | nuoE → | NADH‑quinone oxidoreductasesubunit E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,664,263 | 0 | G | T | 28.4% | 27.5 / 3.6 | 14 | K40N (AAG→AAT) | nuoE | NADH‑quinone oxidoreductasesubunit E |
| Reads supporting (aligned to +/- strand): ref base G (4/6); new base T (2/2); total (6/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGACCGCTAAGCATGAACAGCACGCTTATCCAGACAGACCGTTTCGCCCTGAGCGAAACCGAGCGCTCGGCCATCGAGCACGAAATGCATCACTACGAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATCGGCGAAGTGCTGGGCATTCCGGCCAGCGACGTCGAGGGTGTCGCCACCTTCTACAGCCA > NC_002947/4664131‑4664373 | tGGACCGCTAAGCATGAACAGCACGCTTATCCAGACAGACCGTTTCGCCCTGAGCGAAACCGAGCGCTCGGCCATCGAGCACGAAATGCATCACTACGAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATc < 2:68084/136‑1 (MQ=255) aaCAGCACGCTTATCCAGACAGACCGTTTCGCCCTGAGCGAAACCGAGCGCTCGGCCATCGAGCACGAAATGCATCACTACGAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGGAGCGTg < 1:94758/136‑1 (MQ=255) gAGCGAAACCGAGCGCTCGGCCATCGAGCACGAAATGCATCACAACGAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATCGGc > 1:26082/1‑136 (MQ=255) gcTCGGCCATCGAGCACGAAATGCATCACTACGAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATCGGCGAAGTGCTGGGCAt > 1:125375/1‑136 (MQ=255) gcTCGGCCATCGAGCACGAAATGCATCACTACGAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGGAGCGAGGCTGGGTGCCGGACGGCGCCCTTCACGCCATCGGCGAAGTGCTGGGCAt > 1:19852/1‑136 (MQ=255) tCACTACGAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATCGGCGAAGTGCTGGGCATTCCGGCCAGCGACGTCGAGGGTGTc < 2:257966/136‑1 (MQ=255) acACTACGAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATCGGCGAAGTGCTGGGCATTCCGGCCAGCGACGTCGAGGGTGTc < 1:321873/135‑1 (MQ=255) gAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATCGGCGAAGTGCTGGGCATTCCGGCCAGCGACGTCGAGGGTGTCGCCACCt < 2:33747/136‑1 (MQ=255) aGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATCGGCGAAGTGCTGGGCATTCCGGCCAGCGACGTCGAGGGTGTCGCCACCtt < 1:75161/136‑1 (MQ=255) ggACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATCGGCGAAGTGCTGGGCATTCCGGCCAGCGACGTCGAGGGTGTCGCCACCTTc > 1:298317/1‑136 (MQ=255) aTCGAAGCCCTGAATATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCAGGCCATCGGCGAAGTGCTGGGCATTCCGGCCAGCGACGTCGAGGGTGTCGCCACCTTCTACAGCCa < 2:356936/125‑1 (MQ=255) aTCGAAGCCCTGAATATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATTGGCGAAGTGCTGGGCATTCCGGCCAGCGACGTCGAGGGTGTCGCCACCTTCTACAGCCa > 1:257058/1‑125 (MQ=255) aTCGAAGCCCTGAATATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATTGGCGAAGTGCTGGGCATTCCGGCCAGCGACGTCGAGGGTGTCGCCACCTTCTACAGCCa < 2:257058/125‑1 (MQ=255) aTCGAAGCCCTGAATATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATCGGCGAAGTGCTGGGCATTCCGGCCAGCGACGTCGAGGGTGTCGCCACCTTCTACAGCCa > 1:356936/1‑125 (MQ=255) | TGGACCGCTAAGCATGAACAGCACGCTTATCCAGACAGACCGTTTCGCCCTGAGCGAAACCGAGCGCTCGGCCATCGAGCACGAAATGCATCACTACGAGGACCCGCGCGCGGCGTCCATCGAAGCCCTGAAGATCGTCCAGAAGGAGCGTGGCTGGGTGCCGGACGGCGCCATTCACGCCATCGGCGAAGTGCTGGGCATTCCGGCCAGCGACGTCGAGGGTGTCGCCACCTTCTACAGCCA > NC_002947/4664131‑4664373 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |