| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,813,140 | G→T | 50.3% | intergenic (‑255/‑300) | PP_4234 ← / → dsbD‑II | bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,813,140 | 0 | G | T | 50.3% | ‑6.1 / 12.3 | 12 | intergenic (‑255/‑300) | PP_4234/dsbD‑II | bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2 |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base T (3/3); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01 | |||||||||||
TGTCGCGGTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCAC > NC_002947/4813011‑4813253 | tgtCGCGGTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAg < 2:320129/136‑1 (MQ=255)tgtCGCGGTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGACGGGAGAGTTTCAg > 2:240627/1‑136 (MQ=255) gtgtgtATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTgg > 1:87520/1‑136 (MQ=255) aTGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTTGCCGGAGGATTTTCAGCGCCTGTGAGATCGAGcgccg > 2:316227/1‑136 (MQ=38) aTGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGTGAGATCGAGcgccg > 1:187628/1‑136 (MQ=255) aTGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGcgccg > 1:94636/1‑136 (MQ=255) ggggTAGGTGTTTGGCGGTAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCTAGCGCCGCCCGCGCGGCGCTTCgcgggta < 1:323137/135‑3 (MQ=37) tggcgggAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGgtgttcgccgggagagtttcagcgcctgtgagatcgagcgccgcccgcgcggcgcttcgcgggtaaacccgctccta < 2:12569‑M2/133‑78 (MQ=255) tggcgggAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGgtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcgggtaaacccgctccta < 2:84109‑M2/133‑78 (MQ=255) gttttcAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGgtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcgggtaaacccgctcctacgtttgttt > 1:84109‑M2/2‑50 (MQ=255) ttttCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGgtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcttcgcgggtaaacccgctcctacgtttgtttc > 2:5933‑M2/1‑49 (MQ=255) cTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGgtgttcgccgggagagtttcagcgcctgggagatcgagcgccgcccgcgcggcgcgtcgcgggttaacccgcgccgacggttgtttagggccaattt < 1:266394‑M2/136‑98 (MQ=255) | TGTCGCGGTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCGCGAAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGAAAATCCTCCGGCAAACACCTCAAAATTCCAACGCCGTCCAC > NC_002947/4813011‑4813253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |