| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,468,576 | G→T | 25.0% | H296N (CAC→AAC) | lipA ← | lipoyl synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,468,576 | 0 | G | T | 25.0% | 34.1 / 5.1 | 16 | H296N (CAC→AAC) | lipA | lipoyl synthase |
| Reads supporting (aligned to +/- strand): ref base G (4/8); new base T (2/2); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
GCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGCTGCAGGTACTGGCCGAGGGTGAGCATGTCGATGTCATGCTCGCGCATGCGGTGCATCACTTCGATCACTTCCTCGTCGGTTTCGCCCAGGCCGAG > NC_002947/5468450‑5468708 | gCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACg > 1:151960/1‑136 (MQ=255) tGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTg < 1:160847/136‑1 (MQ=255) cTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTTAACGAAACGCTGCAcc > 1:209018/1‑136 (MQ=255) cTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTTAACGAAACGCTGCAcc > 1:258162/1‑136 (MQ=255) cTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTGCAcc > 2:71438/1‑136 (MQ=255) gctgGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTGCACCGGCAAGTGGCTGCGCGa > 1:77876/1‑136 (MQ=255) gTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGc < 2:77876/136‑1 (MQ=255) ggCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGCTGc < 2:28650/136‑1 (MQ=255) aCGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGCTGCAGGTACTg < 2:12072/136‑1 (MQ=255) aaGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGCTGCAGGTACTGGCCGAGGGTGAGCATGTCGATGTc < 1:171164/136‑1 (MQ=255) aaGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGCTGCAGGTACTGGCCGAGGGTGAGCATGTCGATGTc < 1:249445/136‑1 (MQ=255) tGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGCTGCAGGTACTGGCCGAGGGTGAGCATGTCGATGTCATGCTCGCGCAt < 2:151960/136‑1 (MQ=255) gAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGCTGCAGGTACTGGCCGAGGGTGAGCATGTCGATGTCATGCTCGCGCATg > 1:328618/1‑136 (MQ=255) tgtCCGGGTGAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGCTGCAGGTACTGGCCGAGGGTGAGCATGTCGATGTCATGCTCGCGCATGCGGTGCATCACTTCGATCACTTCCTCGTCGGTTTCGCCCAg < 1:71438/136‑1 (MQ=255) ggTTAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGCTGCAGGTACTGGCCGAGGGTGAGCATGTCGATGTCATGCTCGCGCATGCGGTGCATCACTTCGATCACTTCCTCGTCGGTTTCGCCCAGGCCGAg < 2:209018/136‑1 (MQ=255) ggTTAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGCTGCAGGTACTGGCCGAGGGTGAGCATGTCGATGTCATGCTCGCGCATGCGGTGCATCACTTCGATCACTTCCTCGTCGGTTTCGCCCAGGCCGAg < 2:258162/136‑1 (MQ=255) | GCTTGATCTTGGCTTCGTGGGCCTGCTGGTCGGCGTGGTACGAAGAACGCACCAGCGGGCCAGAAGCAACGTTCTTGAAGCCCATCTTGTAACCTTCCTCGGCGAACCAGGCAAAGGTGTCCGGGTGAACGAAACGCTGCACCGGCAAGTGGCTGCGCGACGGCTGCAGGTACTGGCCGAGGGTGAGCATGTCGATGTCATGCTCGCGCATGCGGTGCATCACTTCGATCACTTCCTCGTCGGTTTCGCCCAGGCCGAG > NC_002947/5468450‑5468708 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |