| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,527,127 | T→C | 15.4% | D93D (GAT→GAC) | PP_4855 → | osmotically‑inducible lipoprotein OsmE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,527,127 | 0 | T | C | 15.4% | 59.7 / 5.3 | 26 | D93D (GAT→GAC) | PP_4855 | osmotically‑inducible lipoprotein OsmE |
| Reads supporting (aligned to +/- strand): ref base T (14/8); new base C (2/2); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CATCGCCATTGGCGGCAGCCCGTCGAGCGTGATCGACCTGCCACACGGGGGCACCTGCAACGACTACATCCTCAACCACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCCTGCTGGAGATAACCATGAGCA > NC_002947/5526995‑5527239 | cATCGCCATTGGCGGCAGCCCGTCGAGCGTGATCGACCTGCCACACGGGGGCACCTGCAACGACTACATCCTCAACCACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGcc > 2:204585/1‑136 (MQ=255) gCCATTGGCGGCAGCCCGTCGAGCGTGATCGACCTGCCACACGGGGGCACCTGCAACGACTACATCCTCAACCACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGACGCCAAAg > 2:387785/1‑136 (MQ=255) gCCATTGGCGGCAGCCCGTCGAGCGTGATCGACCTGCCACACGGGGGCACCTGCAACGACTACATCCTCAACCACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGACGCCAAAg > 2:385834/1‑136 (MQ=255) gCCATTGGCGGCAGCCCGTCGAGCGTGATCGACCTGCCACACGGGGGCACCTGCAACGACTACATCCTCAACCACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCAGCCATGTGGATGCCAAAg > 2:4725/1‑136 (MQ=255) ccGTCGAGCGTGATCGACCTGCCACACGGGGGCACCTGCAACGACTACATCCTCAACCACGAAGGCAAGCAGCAGCCCTAATACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTACAAGACCTgca > 1:260093/1‑136 (MQ=255) actcGGGGGCACCTGCAACGACTACATCCTCAACCACGATGGCAAGCCGCAGCCCTATTACGTGCGCTTCGATGCCACCGGCCAGGGGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGAc < 1:321168/133‑1 (MQ=255) ggCACCTGCAACGACTACATCCTCAACCACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCAGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGc < 1:4725/136‑1 (MQ=255) cTGCAACGACTACATCCTCAACCACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGgc < 1:186470/136‑1 (MQ=255) gCAACGACTACATCCTCAACCACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGgcgc < 2:297322/136‑1 (MQ=255) gACTACATCCTCAACCACGATGGCAAGCAGCAGCCATATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAg < 2:100852/136‑1 (MQ=255) ccACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGc < 1:6498/110‑1 (MQ=255) ccACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGc > 2:6498/1‑110 (MQ=255) cACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTccgaccc > 1:307590/1‑136 (MQ=255) tGGAAAGCAGCAGACCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGAATGGCGCGTAAGCCTGTGTTACgacccgaccc > 1:373696/1‑136 (MQ=255) gcagcagcCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGAccc < 1:264005/136‑1 (MQ=255) agcagcCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCct > 1:400466/1‑136 (MQ=255) agcCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCctgct > 1:287383/1‑136 (MQ=255) cTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGAAAGCCTGTGTTCCGACCCGACCCTGACCCCTGCTGgag > 2:20977/1‑136 (MQ=255) tGCGCTTCGATGCCACCGGCCATGTGGACGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCCTGCTGGAGATAACCAt < 1:387785/136‑1 (MQ=255) tGCGCTTCGATGCCACCGGCCATGTGGACGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCCTGCTGGAGATAACCAt < 1:385834/136‑1 (MQ=255) gcTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCCTGCTGGAGATAACCATGAg > 2:208648/1‑136 (MQ=255) gcTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCCTGCTGGAGATAACCATGAg > 2:324480/1‑136 (MQ=255) gcTTCGATGCCACCGGCCATCTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCCTGCTGGAGATAACCATGAg > 1:96613/1‑136 (MQ=255) ttCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCCTGCTGGAGATAACCATGAGCa > 1:86965/1‑136 (MQ=255) ttCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCCTGCTGGAGATAACCATGAGCa > 1:353009/1‑136 (MQ=255) ttCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCCTGCTGGAGATAACCATGAGCa < 1:204585/136‑1 (MQ=255) | CATCGCCATTGGCGGCAGCCCGTCGAGCGTGATCGACCTGCCACACGGGGGCACCTGCAACGACTACATCCTCAACCACGATGGCAAGCAGCAGCCCTATTACGTGCGCTTCGATGCCACCGGCCATGTGGATGCCAAAGGCTTCAAGACCTGCAAGCAACGCCAGGAGGACAGCGACGCCCTGCATGGCGCGTAAGCCTGTGTTCCGACCCGACCCTGACCCCTGCTGGAGATAACCATGAGCA > NC_002947/5526995‑5527239 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |