| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,677,577 | C→A | 23.5% | intergenic (+38/‑181) | PP_4979 → / → rhlE‑II | substrate‑binding protein/ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,677,577 | 0 | C | A | 23.5% | 34.0 / 5.1 | 17 | intergenic (+38/‑181) | PP_4979/rhlE‑II | substrate‑binding protein/ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base C (8/5); new base A (2/2); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01 | |||||||||||
GCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTCCGTTTCCCGGATGTGCAGTGAAAGCCCAGCTGGACCGGACGCGATCGCATTTCACCGATGC > NC_002947/5677459‑5677699 | gCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCa < 1:301633/136‑1 (MQ=255) aaCCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATc > 1:137454/1‑136 (MQ=255) aaCCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATc > 2:190466/1‑136 (MQ=255) ccGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATccc < 1:394936/136‑1 (MQ=255) acacTGGAAACATTCTCAACGCCGTTGAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACg > 1:149590/1‑136 (MQ=255) acacTGGAAACATTCTCAACGCCGTTGAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACg > 1:24939/1‑136 (MQ=255) aaCATTCTCAACGCCGTTGAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTcg > 2:239049/1‑136 (MQ=255) cGCCGTTGAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCAGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTc > 2:37875/1‑136 (MQ=255) cGCCGTTGAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCAGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTc > 2:339834/1‑136 (MQ=255) gAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTCCGTTTcc < 2:149590/136‑1 (MQ=255) gAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTCCGTTTCCCGGATGTGc < 1:248224/136‑1 (MQ=255) ccTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTCCGTTTCCCGGATGTGCAGTGAAAGCCCAGCTGGACCGGACGCGATCGCATTTc > 2:279910/1‑136 (MQ=255) ccTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTCCGTTTCCCGGATGTGCAGTGAAAGCCCAGCTGGACCGGACGCGATCGCATTTc > 1:128828/1‑136 (MQ=255) ttCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTCCGTTTCCCGGATGTGCAGTGAAAGCCCAGCTGGACCGGACGCGATCGCATTTCAc > 1:180156/1‑136 (MQ=255) cccgGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTCCGTTTCCCGGATGTGCAGTGAAAGCCCAGCTGGACCGGACGCGATCGCATTTCACCGATGc < 1:173292/136‑1 (MQ=255) cccgGCTCACGCCAGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTCCGTTTCCCGGATGTGCAGTGAAAGCCCAGCTGGACCGGACGCGATCGCATTTCACCGATGc < 1:37875/136‑1 (MQ=255) cccgGCTCACGCCAGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTCCGTTTCCCGGATGTGCAGTGAAAGCCCAGCTGGACCGGACGCGATCGCATTTCACCGATGc < 1:339834/136‑1 (MQ=255) | GCGATCAGCAACCGTTATACAACCGACACTGGAAACATTCTCAACGCCGTTGAGCAGCAGGAAAGCAGCACAGCGCGATAGCTCTGTTATACTCGGGCCTTCCCGCCCGGCTCACGCCCGGACGCTCGGCCTCGCAACAGGCATCCCGATCGGTACCGACGCCCCTTCGCGTCCAGCCTCCGTTTCCCGGATGTGCAGTGAAAGCCCAGCTGGACCGGACGCGATCGCATTTCACCGATGC > NC_002947/5677459‑5677699 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |