Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,705,255 G→A 12.1% C243Y (TGC→TAC)  phaA → poly(3‑hydroxyalkanoate) polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,705,2550GA12.1% 89.1 / 4.0 33C243Y (TGC→TAC) phaApoly(3‑hydroxyalkanoate) polymerase
Reads supporting (aligned to +/- strand):  ref base G (18/11);  new base A (2/2);  total (20/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01

CGATGTGCTGGAACTGATCCAGTACAGCCCCATCACCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATGGGGCCTGTCCACCTACATCGATGCGCTGAAAGAAGCCGTCGACGCGGTGCTGTCGATTACCGGCA  >  NC_002947/5705121‑5705386
                                                                                                                                      |                                                                                                                                   
cGATGTGCTGGAACTGATCCAGTACAGCCCCATCACCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTctgc                                                                                                                                    >  1:385410/1‑136 (MQ=255)
        tGGAACTGATCCAGTACAGCCCCATCACCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGAGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTc                                                                                                                            >  2:260186/1‑136 (MQ=255)
        tGGAACTGATCCAGTACAGCCCCATCACCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGATCTACGTGATCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTc                                                                                                                            >  2:110320/1‑136 (MQ=255)
                  ccAGTACAGCCCCATCACCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCgcagcagcac                                                                                                                  >  1:51826/1‑135 (MQ=255)
                    aGTACAGCCCCATCACCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTACCTGCGCTCGCAGCAGCAGAc                                                                                                                >  2:112025/1‑136 (MQ=255)
                    aGTACAGCCCCATCACCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTACCTGCGCTCGCAGCAGCAGAc                                                                                                                >  2:109957/1‑136 (MQ=255)
                        cAGCCCCATCACCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTtc                                                                                                            >  2:75972/1‑136 (MQ=255)
                        cAGCCCCATCACCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCAGCGCTCGCAGCAGCAGACCTtc                                                                                                            >  1:293926/1‑136 (MQ=255)
                              cATCACCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTtcatcatc                                                                                                      >  2:364470/1‑136 (MQ=255)
                                  aCCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCt                                                                                                  >  2:83769/1‑136 (MQ=255)
                                   ccGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTg                                                                                                 >  2:420106/1‑136 (MQ=255)
                                       gCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGgtgc                                                                                             <  1:162774/136‑1 (MQ=255)
                                       gCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGgcgc                                                                                             <  1:420106/136‑1 (MQ=255)
                                              cATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGa                                                                                      <  2:81614/136‑1 (MQ=255)
                                              cATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGa                                                                                      >  1:215876/1‑136 (MQ=255)
                                              cATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGa                                                                                      <  2:385410/136‑1 (MQ=255)
                                                         gctgctGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAg                                                                           >  1:46574/1‑136 (MQ=255)
                                                         gctgctGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAg                                                                           >  1:307861/1‑136 (MQ=255)
                                                         gctgctGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAg                                                                           >  1:13062/1‑136 (MQ=255)
                                                          ctgctgGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGc                                                                          <  1:92453/136‑1 (MQ=255)
                                                            gctgGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGt                                                                        <  2:291045/136‑1 (MQ=255)
                                                             ctgGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTACCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTg                                                                       <  1:109957/136‑1 (MQ=255)
                                                             ctgGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTACCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTg                                                                       <  1:112025/136‑1 (MQ=255)
                                                                  ggtgCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATgg                                                                  <  2:215876/136‑1 (MQ=255)
                                                                       cACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATGGGGCCt                                                             <  1:390063/136‑1 (MQ=255)
                                                                       cACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATGGGGCCt                                                             <  1:297545/136‑1 (MQ=255)
                                                                        aCCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATGGGGCCTg                                                            >  2:111562/1‑136 (MQ=255)
                                                                                       gTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATGGGGCCTGTCCACCTACATCGAt                                             >  2:219502/1‑136 (MQ=255)
                                                                                                gtTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATGGGGCCTGTCCACCTACATCGATGCGCTGaaa                                    >  2:307732/1‑136 (MQ=255)
                                                                                                     aCCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATGGGGCCTGTCCACCTACATCGATGCGCTGAAAGAAGc                               >  2:384999/1‑136 (MQ=255)
                                                                                                     aCCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATGGGGCCTGTCCACCTACATCGATGCGCTGAAAGAAGc                               >  1:32893/1‑136 (MQ=255)
                                                                                                                   aaGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATGGGGCCTGTCCACCTACATCGATGCGCTGAAAGAAGCCGTCGACGCGGTGc                 >  2:234580/1‑136 (MQ=255)
                                                                                                                             cgcgcTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATGGGGCCTGTCCACCTACATCGATGCGCTGAAAGAAGCCGTCGACGCGGTGCTGTCGATTAc       <  2:149948/136‑1 (MQ=255)
                                                                                                                                 cTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGG‑CCAGCGTGAATGGGGCCTGTCCACCTACATCGATGCGCTGAAAGAAGCCGTCGACGCGGTGCTGTCGATTACCGgca  <  1:364470/136‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                   
CGATGTGCTGGAACTGATCCAGTACAGCCCCATCACCGAGCAGGTGCATGCCCGTCCGCTGCTGGTGGTGCCACCGCAGATCAACAAGTTCTACGTGTTCGACCTCAGCCCGGAAAAGAGCCTGGCGCGCTTCTGCCTGCGCTCGCAGCAGCAGACCTTCATCATCAGCTGGCGCAACCCGACCAAGGCCCAGCGTGAATGGGGCCTGTCCACCTACATCGATGCGCTGAAAGAAGCCGTCGACGCGGTGCTGTCGATTACCGGCA  >  NC_002947/5705121‑5705386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑