Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,778,524	G→T	42.5%	G28W (GGG→TGG)	betI →	choline‑responsive transcriptional repressor BetI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,778,524	0	G	T	42.5%	9.6 / 10.1	14	G28W (GGG→TGG)	betI	choline‑responsive transcriptional repressor BetI
Reads supporting (aligned to +/- strand):  ref base G (2/6);  new base T (3/3);  total (5/9)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CTTCGCCGAGTCAGCCGCCTGTCGTCTGCTCGCAGGCCTGAGGAGATAGCAAGATGCCCAAGGTCGGTATGCAACCCATCCGGCGCCAGCAGTTGATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGGGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACGGCATCATCAGTCACTACTTTCGGGACAAGAACGGCCTGATCGCAGCGACGATGGGTTACA  >  NC_002947/5778390‑5778632                                                                                                                                       |                                                                                                             cTTCGCCGAGTCAGCCGCCTGTCGTCTGCTCGCAGGCCTGAGGAGATAGCAAGATGCCCAAGGTCGGTATGCAACCCATCCGGCGCCAGCAGTTGATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTggg                                                                                                             >  1:86607/1‑136 (MQ=255)         aGTCAGCCGCCTGTCGTCTGCTCGCAGGCCTGAGGAGATAGCAAGATGCCCAAGGTCGGTATGCAACCCATCCGGCGCCAGCAGTTGATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGGGGGACGCCa                                                                                                     <  2:111690/136‑1 (MQ=255)              gccgccTGTCGTCTGCTCGCAGGCCTGAGGAGATAGCAAGATGCCCAAGGTCGGTATGCAACCCATCCGGCGCCAGCAGTTGATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGGGGGACGCCAGCAtt                                                                                                <  2:142865/136‑1 (MQ=255)                                                 gCAAGATGCCCAAGGTCGGTATGCAACCCATCCGGCGCCAGCAGTTGATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGTGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACgg                                                             >  2:283295/1‑136 (MQ=255)                                                 gCAAGATGCCCAAGGTCGGTATGCAACCCATCCGGCGCCAGCAGTTGATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGTGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACgg                                                             >  2:412502/1‑136 (MQ=255)                                                 gCAAGATGCCCAAGGTCGGTATGCAACCCATCCGGCGCCAGCAGTTGATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGTGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACgg                                                             >  2:421276/1‑136 (MQ=255)                                                        gCCCAAGGTCGGTATGCAACCCATCCGGCGCCAGCAGTTGATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGGGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACGGCatcatc                                                      <  1:396469/136‑1 (MQ=255)                                                            aaGGTCGGTATGCAACCCATCCGGCGCCAGCAGTTGATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGGGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACGGCATCATCAGTc                                                  >  1:138362/1‑136 (MQ=255)                                                                 cGGTATGCAACCCATCCGGCGCCAGCAGTTGATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGGGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACGGCATCATCAGTCactac                                             <  2:86607/136‑1 (MQ=255)                                                                                               gATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGTGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACGGCATCATCAGTCACTACTTTCGGGACAAGAACGGCCTGATCGCAGcg               <  1:412502/136‑1 (MQ=255)                                                                                               gATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGTGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACGGCATCATCAGTCACTACTTTCGGGACAAGAACGGCCTGATCGCAGcg               <  1:421276/136‑1 (MQ=255)                                                                                               gATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGTGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACGGCATCATCAGTCACTACTATCGGGACAAGAACGGCCTGATCGCAGcg               <  1:283295/136‑1 (MQ=255)                                                                                                      cccACATTGCAGGCGGTCGATCAGGTCGGACTGGGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACGGCATCATCAGTCACTACTTTCGGGACAAGAACGGCCTGATCGCAGCGACGATgg        <  2:36787/135‑1 (MQ=255)                                                                                                                        gATCAGGTCGGACTGGGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACGGCATCATCAGTCACTACTTTCGGGACAAGAACGGCCTGATCGCAGCGACGATGGGTTAca  >  1:394728/1‑124 (MQ=255)                                                                                                                        gATCAGGTCGGACTGGGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACGGCATCATCAGTCACTACTTTCGGGACAAGAACGGCCTGATCGCAGCGACGATGGGTTAca  <  2:394728/124‑1 (MQ=255)                                                                                                                                       |                                                                                                             CTTCGCCGAGTCAGCCGCCTGTCGTCTGCTCGCAGGCCTGAGGAGATAGCAAGATGCCCAAGGTCGGTATGCAACCCATCCGGCGCCAGCAGTTGATCGAAGCCACATTGCAGGCGGTCGATCAGGTCGGACTGGGGGACGCCAGCATTGCGCTGATTGCCCGTTTGGCCGGTGTGTCGAACGGCATCATCAGTCACTACTTTCGGGACAAGAACGGCCTGATCGCAGCGACGATGGGTTACA  >  NC_002947/5778390‑5778632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑