| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,945,268 | G→T | 25.0% | V34V (GTG→GTT) | yhhJ → | translation‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,945,268 | 0 | G | T | 25.0% | 28.8 / 5.1 | 16 | V34V (GTG→GTT) | yhhJ | translation‑like protein |
| Reads supporting (aligned to +/- strand): ref base G (5/7); new base T (2/2); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01 | |||||||||||
CTGGCAATGCTGAAAAAGCAGGAGGCCTGACATGTCGCGCCTGAACCACACCCTGCGCCTGGGCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAACGCCAGCGTGGCGGTGGTGGATGAAGACCATAGCCTGCTCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGC > NC_002947/5945136‑5945383 | cTGGCAATGCTGAAAAAGCAGGAGGCCTGACATGTCGCGCCTGAACCACACCCTGCGCCTGGGCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCg < 2:163831/136‑1 (MQ=255) aaTGCTGAAAAAGCAGGAGGCCTGACATGTCGCGCCTGAACCACACCCTGCGCCTGGGCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTa > 2:38037/1‑136 (MQ=255) aggaggCCTGACATGTCGCGCCTGAACCACACCCTGCGCCTGGGCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCg < 2:236137/136‑1 (MQ=255) aggCCTGACATGTCGCGCCTGAACCACACCCTGCGCCTGGGCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCGGCt > 1:46549/1‑136 (MQ=255) ccTGACATGTCGCGCCTGAACCACACCCTGCGCCTGGGCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCGGCTCgg > 1:219846/1‑136 (MQ=255) ccTGACATGTCGCGCCTGAACCACACCCTGCGCCTGGGCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCGGCTCgg < 2:46549/136‑1 (MQ=255) gAACCACACCCTGCGCCTGGGCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTTGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAAc > 2:142093/1‑136 (MQ=255) gAACCACACCCTGCGCCTGGGCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTTGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAAc > 2:74672/1‑136 (MQ=255) gCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAACGCCAGCGTGGCGGTGGTGGa < 2:29678/136‑1 (MQ=255) gCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAACGCCAGCGTGGCGGTGGTGGa < 2:388587/136‑1 (MQ=255) gCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAACGCCAGCGTGGCGGTGGTGGa < 2:99836/136‑1 (MQ=255) ccAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTTGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAACGCCAGCGTGGCGGTGGTGGATGAAGACCATAGCCt < 1:142093/136‑1 (MQ=255) ccAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTTGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAACGCCAGCGTGGCGGTGGTGGATGAAGACCATAGCCt < 1:74672/136‑1 (MQ=255) aGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAACGCCAGCGTGGCGGTGGTGGATGAAGACCATAGCCTGc > 1:388624/1‑136 (MQ=255) aGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAACGCCAGCGTGGCGGTGGTGGATGAAGACCATAGCCTGCTCTCGCGCAAGCTCa < 2:111819/136‑1 (MQ=255) cTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAACGCCAGCGTGGCGGTGGTGGATGAAGACCATAGCCTGCTCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGc > 1:162292/1‑136 (MQ=255) | CTGGCAATGCTGAAAAAGCAGGAGGCCTGACATGTCGCGCCTGAACCACACCCTGCGCCTGGGCCTGAAAGAACTGACCAGCCTGCGCCATGACAGCGTCCTGCTGCTGTTCCTGCTATATGCCTTCAGCGTGGCGATCTACATGCCGGCAGCCGGCTCGGTGATCGGCGTACACAACGCCAGCGTGGCGGTGGTGGATGAAGACCATAGCCTGCTCTCGCGCAAGCTCAGCGAAGCCCTGCAACCGC > NC_002947/5945136‑5945383 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |