| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,993,814 | G→A | 33.3% | intergenic (+103/‑443) | kefB‑III → / → PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,993,814 | 0 | G | A | 33.3% | 28.3 / 20.6 | 27 | intergenic (+103/‑443) | kefB‑III/PP_mr64 | glutathione‑regulated potassium/H+ antiporter/C4 ncRNA |
| Reads supporting (aligned to +/- strand): ref base G (4/14); new base A (4/5); total (8/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.64e-01 | |||||||||||
CTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGC > NC_002947/5993679‑5993892 | ctggagcGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAg < 2:277430/136‑1 (MQ=255) gcgttggaattttgagtggacggcgttggaattttgaggtgtttgccggaggattttcagcgcctgtgagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTC‑GGccagtccct > 1:372720‑M2/102‑132 (MQ=255) gcgttggaattttgagtggacggcgttggaattttgaggtgtttgccggaggattttcagcgcctgtgagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTC‑GGccagtcccg > 1:116078‑M2/102‑132 (MQ=255) gcgttggaattgtgagtggacggcgttggaattttgaggggtttgccggaggattttcagcgcctgtgagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 2:108821‑M2/35‑1 (MQ=255) aTGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCAcg < 2:201203/136‑1 (MQ=255) ggCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTg < 1:222784/136‑1 (MQ=255) ccTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGgc < 1:279169/136‑1 (MQ=255) gggTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGcgtc < 1:376029/136‑1 (MQ=255) cctgtgagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 2:317100‑M2/35‑1 (MQ=255) cctgtgagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt > 2:242345‑M2/40‑74 (MQ=255) cctgtgagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt > 2:323313‑M2/40‑74 (MQ=255) cctgtgagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 2:38518‑M2/35‑1 (MQ=255) cctgtgagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt > 1:38518‑M2/40‑74 (MQ=255) cctgtgagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 1:323313‑M2/35‑1 (MQ=255) cctgtgagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt > 1:317100‑M2/40‑74 (MQ=255) cctgtgagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAATTAt < 1:242345‑M2/35‑1 (MQ=255) ggagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTAt > 1:267427‑M2/36‑70 (MQ=255) ggagatcgagcgccgcccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTAt < 2:267427‑M2/35‑1 (MQ=255) gaTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcg < 1:421158/136‑1 (MQ=255) gaTTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcg < 1:308749/136‑1 (MQ=255) ggggCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgc < 2:232909/135‑1 (MQ=255) ggCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgc < 1:314847/136‑1 (MQ=255) ggCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgc < 2:109479/136‑1 (MQ=255) ggCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTcgcgcgc < 1:156394/136‑1 (MQ=255) ccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGtt < 2:162732‑M2/33‑1 (MQ=255) ccgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGtt > 1:162732‑M2/20‑52 (MQ=255) cgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGtt < 2:401172‑M2/33‑1 (MQ=255) cgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGtt < 2:158326‑M2/33‑1 (MQ=255) cgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGtt > 1:401172‑M2/19‑51 (MQ=255) cgcgcggcgcttcgcgggTAAACCCGCTCCTACGTTTGTTTCGGGCCAGtt > 1:158326‑M2/19‑51 (MQ=255) | CTGGAGCGGTTGTTTGATTCGGATGCGGACTGAGGCCTTTTGGCGCGTGCCTTGAGGGTTGTAGTGCCTGTGAGATTGGGCGCCGCCCGCGCGGCGCTTCGCGGGTAAACCCGCTCCTACGTTTGTTTCGGGCCAGTTATTCCTGTGGGACGCGCACGCGAGCCCCCTGGCGCACGCCTCGATATCGCGTCGTACAAACAAGGCGGTCGCGCGC > NC_002947/5993679‑5993892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |