| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,749,651 | 0 | A | T | 53.8% | 1.5 / 10.2 | 13 | I315F (ATT→TTT) | paoB | aromatic aldehyde dehydrogenase FAD‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base T (0/7); total (2/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.70e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCGGTTGTGGAGCGCATGCTGGCCGGCCAGGCGCCTTGCGAAGCGCTGTTCACCCGGGCTGCAAAAACGTTGCTGCAAGGTGCAAAACCTCTGAGCCACAACGGCTTCAAGGTCACGCTCGCCCAACGTG‑CAATTGTGCGGGCATTGGATGACGCGGCGCGCGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCGGTACGTCCCTGGACCGCGTAGACGGCGTGGCGAAGGTCAC > NC_002947/3749519‑3749771 | ccGGTTGTGGAGCGCATGCTGGGCGGCCAGGCGCCTTGCGAAGCGCTGTTCACCCGGGCTGCAAAAACGTTGCTGCAAGGTGCAAAACCTCTGAGCCACAACGGCTTCAAGGTCACGCTCGCCCAACGTG‑CAATtg < 2:521439/136‑1 (MQ=255) gAAGCGCTGTTCACCCGGGCTGCAAAAACGTTGCTGCAAGGTGCAAAACCTCTGAGCCACAACGGCTTCAAGGTCACGCTCGCCCAACGTG‑CATTTGTGCGGGCATTGGATTCCGCGGCGCGCGGCGCCGCTGACg < 1:197365/136‑1 (MQ=255) tggTTCAAAACCTCTGAGCCACATGGGGTTCATGGTCACGCTCGCCCAAGGTG‑CATTTGGGGGGTTTTTGTTTCCGGCGGCGCGGGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCg < 2:365754/135‑1 (MQ=255) tggTTCAAAACCTCTCAGCCAATAGGGTTTCTTGGTCACGCTCGCCCACCGTT‑CAATTGTGGGGGCTTTGGTTGCGGGGGGGCGCGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCg < 2:31202/135‑1 (MQ=255) tggTGCAAACCCTCTGAGCCACACCGGCTTCAAGGCCCCGCGCCCCCACCGGG‑CATTGGGGCGGGCTTTGGTTGCCGCGGCGCGCGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCg < 1:288220/135‑1 (MQ=255) tggTGCAAAACCTCTGAGCCACACGGGCTTCATGGTCACGCTCGCCCAACGTG‑CAATTGTGCGGGCTTTGGTTGAGGCGGGGCGCGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCg < 1:114581/135‑1 (MQ=255) cgtggCAAAACTTCTGCGCAACACCGGCTTCATGGGCACGCGCGCCCAA‑GTGTCATTTGTGCGGGCATTGGTCGCGGCGGGGCGGGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCg < 1:273271/132‑1 (MQ=255) cggTGCAAAACCTCTGCCCCACAACGGCTTCAAGGTCACGCCCGCCAACGGTG‑CTATTGGGCGGGCATTGGATGCCGCGGCGCGGGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCg < 2:98878/135‑1 (MQ=255) aGGTGCAAAACCTCTGAGCCACAACGGCTTCAAGGTCACGCTCGCCCAACGTG‑CATTTGTGCGGGCATTGGATGCCGCGGCGCGCGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCg < 1:105831/136‑1 (MQ=255) aGGTCCAAAACCTCTGACCCACAACGGCTTCAAGGTCACGCTCGCCCAACGTG‑CATTTGTGGGGGCATTGGATGCGGCGGCGCGCGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCg < 1:10263/136‑1 (MQ=255) cccTCTGACCCCCAACGGCTTCACGGCCCCCCCCCCCCACCGTC‑CATTTGTGCGGCCTTGGGTCGCCGCGGCGCGGGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCGGTACGTccc < 1:228289/135‑1 (MQ=255) aGGTCACGCTCGCCAAACGTG‑CAATTGTGCGGGCATTGGATGACGCGGCGCGCGGCGCCGCTGCCGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCGGTACGTCCCTGGACCGCGTAGACGGCGAGGCg > 1:267967/1‑136 (MQ=255) cTCGCCCAACGTG‑CAATTGTGCGGGCATTGGATGACGCGGCGCGCGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCGGTACGTCCCTGGACCGCGTAGACGGCGTGGCGAAGGTCAc > 2:389046/1‑136 (MQ=255) | CCGGTTGTGGAGCGCATGCTGGCCGGCCAGGCGCCTTGCGAAGCGCTGTTCACCCGGGCTGCAAAAACGTTGCTGCAAGGTGCAAAACCTCTGAGCCACAACGGCTTCAAGGTCACGCTCGCCCAACGTG‑CAATTGTGCGGGCATTGGATGACGCGGCGCGCGGCGCCGCTGACGGGGGGCCACGACCATGATCAAACCCACACCGGTAGCCGGTACGTCCCTGGACCGCGTAGACGGCGTGGCGAAGGTCAC > NC_002947/3749519‑3749771 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |