| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 539,301 | A→G | H559R (CAC→CGC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 539,301 | 0 | A | G | 91.7% | 32.9 / ‑3.7 | 12 | H559R (CAC→CGC) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (4/7); total (5/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTGAAGGAAGGCG > NC_002947/539169‑539432 | agcTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCGCTa < 1:413322/136‑1 (MQ=255) tACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCGCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATc < 1:87016/136‑1 (MQ=255) acaAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCGCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCt < 2:414101/136‑1 (MQ=255) gccgcGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCGCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCaa > 1:338681/1‑136 (MQ=255) gTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCGCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTccc < 2:454536/136‑1 (MQ=255) cGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCGCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTggcg < 1:106469/136‑1 (MQ=255) tCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCGCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTa > 1:408544/1‑136 (MQ=255) cGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCGCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCacc < 2:338681/136‑1 (MQ=255) acGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGCACAGACTAACGAATACGGCTTc > 2:335876/2‑136 (MQ=255) ggCCGGCTTCGAAGTCCGTGACGTACACCCGACCCGCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTg < 2:84301/135‑1 (MQ=255) gACGTACACCCGACCCGCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCgtgg > 2:38911/1‑136 (MQ=255) aCCCGCTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTGAAGGAAGGCg > 1:384102/1‑136 (MQ=255) | AGCTGTCCCAGTTCATGGACCAGAACAACCCTCTCTCGGAGATTACCCACAAGCGCCGCGTCTCCGCACTCGGCCCTGGCGGTCTGACCCGTGAGCGTGCCGGCTTCGAAGTCCGTGACGTACACCCGACCCACTATGGCCGTGTGTGCCCGATCGAGACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCCCTGGCGGCCTATGCCCGCACCAACCAGTACGGCTTCCTGGAAAGCCCGTACCGCGTGGTGAAGGAAGGCG > NC_002947/539169‑539432 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |