Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,856,513	A→G	D203G (GAT→GGT)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,856,513	0	A	G	100.0%	91.3 / NA	25	D203G (GAT→GGT)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/13);  total (12/13)

CTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGC  >  NC_002947/1856386‑1856644                                                                                                                                 |                                                                                                                                    cTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTGCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTT‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCa                                                                                                                             >  1:240332/1‑136 (MQ=255)          cTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGc                                                                                                                    <  1:180977/136‑1 (MQ=255)               cGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGaa                                                                                                               <  2:414906/136‑1 (MQ=255)                gAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAg                                                                                                              <  1:267428/136‑1 (MQ=255)                 aaCGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACCGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAg                                                                                                              <  1:11113/136‑1 (MQ=255)                 aaCGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGa                                                                                                             >  1:462030/1‑136 (MQ=255)                      aCGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGt                                                                                                        >  1:510244/1‑136 (MQ=255)                          gcgcAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCtt                                                                                                    <  2:462030/136‑1 (MQ=255)                                tCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTa                                                                                              <  1:424043/136‑1 (MQ=255)                                   gggCGGTCAAGTCCGCCGATGCCGAGAAACGCCTGCGTGTCGCGCGTCAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCt                                                                                           >  1:167832/1‑136 (MQ=255)                                        gTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGc                                                                                      >  1:329186/1‑136 (MQ=255)                                        gTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGc                                                                                      >  1:95264/1‑136 (MQ=255)                                         tCAAGTCCGTCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGcc                                                                                     <  2:4793/136‑1 (MQ=255)                                         tCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGcc                                                                                     <  2:10386/136‑1 (MQ=255)                                          cAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCTCCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGccc                                                                                    <  1:453206/136‑1 (MQ=255)                                           aaGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCg                                                                                   <  2:329186/136‑1 (MQ=255)                                                                    tGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCt                                                          >  1:388441/1‑136 (MQ=255)                                                                                           gACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGcc                                                                                     <  1:35432/86‑1 (MQ=255)                                                                                           gACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGcc                                                                                     >  2:35432/1‑86 (MQ=255)                                                                                                      cgccgcTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCtt                         <  2:510244/136‑1 (MQ=255)                                                                                                           cTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTtcatca                    >  2:294164/1‑136 (MQ=255)                                                                                                           cTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTtcatca                    >  2:16146/1‑136 (MQ=255)                                                                                                                       cTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGtgatga        <  1:324288/136‑1 (MQ=255)                                                                                                                             aTCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGc  >  1:140269/1‑136 (MQ=255)                                                                                                                             aTCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGc  >  1:136236/1‑136 (MQ=255)                                                                                                                                 |                                                                                                                                    CTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTA‑CGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGC  >  NC_002947/1856386‑1856644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑