Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,011,323	+T	intergenic (‑258/+192)	PP_0871 ← / ← SBRG_Ecoli_galM	glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,011,323	1	.	T	100.0%	67.4 / NA	20	intergenic (‑258/+192)	PP_0871/SBRG_Ecoli_galM	glycine betaine/carnitine/choline ABC transporter permease/SBRG_Ecoli_galM
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (12/8);  total (12/8)

CCAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATG‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATA  >  NC_002947/1011197‑1011438                                                                                                                                |                                                                                                                    ccAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAatc                                                                                                             >  1:194888/1‑136 (MQ=255)                      ggAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATtc                                                                                        <  2:194888/136‑1 (MQ=255)                                     gTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGATAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCcgc                                                                         <  1:813157/136‑1 (MQ=255)                                     gTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCcgc                                                                         >  1:581205/1‑136 (MQ=255)                                            tAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAg                                                                  >  2:669709/1‑136 (MQ=255)                                                 gTTCCCTTCTGAGGGGGTTTTTGGCATGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGATAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATTATTCTCATTATATTGCCGCGACGAAGCAACa                                                             <  1:826926/136‑1 (MQ=255)                                                                     ttGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCa                                         >  2:796007/1‑136 (MQ=255)                                                                      tGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCAt                                        >  2:862461/1‑136 (MQ=255)                                                                                   tgggtGGGTTGCGGGTTTTTGGTCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg                           <  2:455084/132‑1 (MQ=255)                                                                                   tCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg                           <  1:264836/136‑1 (MQ=255)                                                                                   ccGCTGGGTTGCTGGTTTTTGGGTGACAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTg                           <  2:172888/135‑1 (MQ=255)                                                                                          gTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAgt                    >  2:38005/1‑136 (MQ=255)                                                                                          gTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAgt                    >  2:745368/1‑136 (MQ=255)                                                                                          gTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAgt                    >  2:675090/1‑136 (MQ=255)                                                                                          gTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAgt                    >  2:220514/1‑136 (MQ=255)                                                                                          gTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAgt                    >  2:163616/1‑136 (MQ=255)                                                                                            tGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAAc                                           <  1:945464/111‑1 (MQ=255)                                                                                            tGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAAc                                           >  2:945464/1‑111 (MQ=255)                                                                                                      tGGGCGAGAAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCAGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGttgttg        >  2:608241/1‑136 (MQ=255)                                                                                                            agaAAAACAGCGACTTGATGTCTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAata  <  2:581205/136‑1 (MQ=255)                                                                                                                                |                                                                                                                    CCAACCGAAGCGGGTTGCAGGGGAGTGGACGTCTCCGTGGGCGTAAAGGTTCCCATCTGAGGCGGCATTTGGCCAGTGTTAATCGCTGGGTTGCGGGTTTTTGGGCGAGAAAAACAGCGACTTGATG‑CTCAAATCATCTTTTAATGATAATAATTCTCATTATATTGCCGCGACGAAGCAACAGCAATGCTTACGCATAACCATAGCGAAAATAGTGGCGCAGTGTAAGGTTGTTGTGAATA  >  NC_002947/1011197‑1011438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑