Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,536,117	C→T	V385M (GTG→ATG)	braE ←	high‑affinity branched‑chain amino acid ABC transporter permease BraE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,536,117	0	C	T	100.0%	110.8 / NA	32	V385M (GTG→ATG)	braE	high‑affinity branched‑chain amino acid ABC transporter permease BraE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/10);  total (22/10)

CACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCACGACGATGGCGAGGATCAGCGCCGACTCGAAAAATGTGAACGACGACGGGTTGACGAAGCCCTGGTAGGTGGCGAAGAAC  >  NC_002947/5535989‑5536244                                                                                                                                 |                                                                                                                                cacaCCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAAcgcc                                                                                                                          >  2:233807/1‑136 (MQ=255) cacaCCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAAcgcc                                                                                                                          >  1:874248/1‑136 (MQ=255) cacaCCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAAcgcc                                                                                                                          >  1:484092/1‑136 (MQ=255) cacaCCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAAcgcc                                                                                                                          >  1:85094/1‑136 (MQ=255) cacaCCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAAcgcc                                                                                                                          >  1:621142/1‑136 (MQ=255)        gTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGa                                                                                                                   <  2:99280/136‑1 (MQ=255)        gTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGa                                                                                                                   <  1:80203/136‑1 (MQ=255)        gTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGa                                                                                                                   <  2:457105/136‑1 (MQ=255)           cGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGAccc                                                                                                                >  2:218513/1‑135 (MQ=255)           cGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGACAAGCTGCGCAGCAGTTCCGGCGCCACGCTCAGCATGAACGCCGCGATGAccc                                                                                                                >  2:569327/1‑135 (MQ=255)             gCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACcaca                                                                                                              <  1:523311/136‑1 (MQ=255)                   aCGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCCCGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCCCGGTCAGCATGAACGCCGCGATGCCCACACCCACg                                                                                                        >  1:217264/1‑136 (MQ=255)                   aCGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACg                                                                                                        >  1:509392/1‑136 (MQ=255)                                cgcgGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGc                                                                                           <  2:874248/136‑1 (MQ=255)                                    gTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCAcc                                                                                       >  1:359258/1‑136 (MQ=255)                                         cAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCCCCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCCCCCAGcc                                                                                  >  2:658100/1‑135 (MQ=255)                                         cAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCa                                                                                  >  1:310575/1‑136 (MQ=255)                                         cAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCa                                                                                  >  2:3402/1‑136 (MQ=255)                                           gATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCacg                                                                                <  2:641645/136‑1 (MQ=255)                                                               gcacGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGCGCCCATGCCACCCAGCACGACGATGGCGAGGATCAgcgc                                                            >  1:339233/1‑136 (MQ=255)                                                                        aCAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCACGACGATGGCGAGGATCAGCGCCGACTCGaa                                                   >  2:706968/1‑136 (MQ=255)                                                                                       aCTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCACGACGATGGCGAGGATCAGCGCCGACTCGAAAAATGTGAacgacga                                    >  2:200201/1‑136 (MQ=255)                                                                                       aCTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCACGACGATGGCGAGGATCAGCGCCGACTCGAAAAATGTGAacgacga                                    >  2:445481/1‑136 (MQ=255)                                                                                        cTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCACGACGATGGCGAGGATCAGCGCCGACTCGAAAAATGTGAacgacgac                                   >  1:494194/1‑136 (MQ=255)                                                                                                aGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCACGACGATGGCGAGGATCAGCGCCGACTCGAAAAATGTGAACGACGACGGGTTGAc                           >  1:799326/1‑136 (MQ=255)                                                                                                        agcagTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCACGACGATGGCGAGGATCAGCGCCGACTCGAAAAATGTGAACGACGACGGGTTGACGAAGCCCt                   >  2:826780/1‑136 (MQ=255)                                                                                                        agcagTTCCGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCACGACGATGGCGAGGATCAGCGCCGACTCGAAAAATGTGAACGACGACGGGTTGACGAAGCCCt                   <  2:845896/136‑1 (MQ=255)                                                                                                                cGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCa                                                                                     <  1:534260/62‑1 (MQ=255)                                                                                                                cGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCa                                                                                     >  2:534260/1‑62 (MQ=255)                                                                                                                cGGCGCCACGGTCAGCATGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCACGACGATGGCGAGGATCAGCGCCGACTCGAAAAATGTGAACGACGACGGGTTGACGAAGCCCTGGTAGGTg           >  1:522401/1‑136 (MQ=255)                                                                                                                    gcCACGGCCCGCATCACCGCCGCGAGGCCCCCACCCCGGGCCGAGCCCATGCCACCCCGAACGACGATGGCGAGGATCCGCCCCGACTCTAAAAATGTTAACGACGCCGGGTTGACGAAGCCCTGGTAGGTGGCga       <  2:813947/136‑1 (MQ=255)                                                                                                                         gggcAGCATGAACGCGGCGATGACCACACCCACGGGCGAGCCCAGGCCACCCACCACGACGATGGCGAGGATCCGCGCCGACTCGAAAAATGTGAACGACGACGGGTTGACGAAGCCCTGGTAGGTGGCGAAGAac  <  1:43145/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                                CACACCGGTACGGCTGATACGGATCAGCCCACGCGGTCGCCAGATCATCATCAGCACCATCAGCACGCCGAACAGCAGCACCCGGTACTCGGAGAAGCTGCGCAGCAGTTCCGGCGCCACGGTCAGCACGAACGCCGCGATGACCACACCCACGGTCGAGCCCATGCCACCCAGCACGACGATGGCGAGGATCAGCGCCGACTCGAAAAATGTGAACGACGACGGGTTGACGAAGCCCTGGTAGGTGGCGAAGAAC  >  NC_002947/5535989‑5536244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑