Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,856,513	A→G	D203G (GAT→GGT)	relA →	ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,856,513	0	A	G	100.0%	106.6 / NA	29	D203G (GAT→GGT)	relA	ATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/19);  total (10/19)

TGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGC  >  NC_002947/1856381‑1856644                                                                                                                                     |                                                                                                                                    gggCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTca                                                                                                                                  <  1:173957/135‑1 (MQ=255)          tCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTg                                                                                                                         <  2:603413/136‑1 (MQ=255)                                        gggCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGTGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCt                                                                                           >  2:346126/1‑136 (MQ=255)                                          gCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTgg                                                                                         <  2:602892/136‑1 (MQ=255)                                             gTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGc                                                                                      <  2:100525/136‑1 (MQ=255)                                              tCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGcc                                                                                     <  1:397161/136‑1 (MQ=255)                                               cAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGccc                                                                                    <  2:465923/136‑1 (MQ=255)                                               aaaGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGccc                                                                                    <  2:236606/135‑1 (MQ=255)                                                            gACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTAGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGc                                                                       <  2:97142/136‑1 (MQ=255)                                                             aCGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCa                                                                      <  1:125560/136‑1 (MQ=255)                                                             aCGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCa                                                                      >  1:406388/1‑136 (MQ=255)                                                             aCGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCa                                                                      >  1:919888/1‑136 (MQ=255)                                                                agaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGAt                                                                   >  2:904210/1‑136 (MQ=255)                                                                 gaAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATc                                                                  <  2:842422/136‑1 (MQ=255)                                                                 caaaCGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATc                                                                  <  2:328353/135‑1 (MQ=255)                                                                    aCGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCATCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGcc                                                               <  1:134033/136‑1 (MQ=255)                                                                        cTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGc                                                           <  1:505810/136‑1 (MQ=255)                                                                            gtgtCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACATGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGtt                                                       >  1:441404/1‑136 (MQ=255)                                                                                         aGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGACCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGcggcgg                                          >  2:171893/1‑136 (MQ=255)                                                                                                gACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCaa                                                             <  1:800749/110‑1 (MQ=255)                                                                                                gACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCaa                                                             >  2:800749/1‑110 (MQ=255)                                                                                                  cATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACcgc                                 <  2:362941/136‑1 (MQ=255)                                                                                                  cATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACcgc                                 <  1:273884/136‑1 (MQ=255)                                                                                                          cgccgcTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCtt                         <  1:550246/136‑1 (MQ=255)                                                                                                               cTCGCGCACCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTtcatca                    >  1:488945/1‑136 (MQ=255)                                                                                                                      aCCGGCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGt             <  2:201959/136‑1 (MQ=255)                                                                                                                      aCCGCCTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGGGGCTGGACCGCGAGCGCTTCATCAGCGACGt             <  2:490563/136‑1 (MQ=255)                                                                                                                          ccTGGGTATCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGCCGtgatg         >  1:243620/1‑136 (MQ=255)                                                                                                                                 aTCGGTCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGc  >  2:846408/1‑136 (MQ=255)                                                                                                                                     |                                                                                                                                    TGGCGCTGATCAAGCTGGCCGAACGCACGTGCGCAATCCGGGCGGTCAAGTCCGCCGATGACGAGAAACGCCTGCGTGTCGCGCGTGAAGTGTTCGACATCTACGCGCCGCTCGCGCACCGCCTGGGTATCGATCACATCAAGTGGGAGCTGGAAGACCTGTCCTTCCGCTACCTGGAGCCCGACCAGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGC  >  NC_002947/1856381‑1856644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑