Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,154,508	T→C	D335G (GAC→GGC)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,154,508	0	T	C	100.0%	161.1 / NA	43	D335G (GAC→GGC)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/15);  total (28/15)

AACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGACACCAGGGCTTCGGTC  >  NC_002947/2154393‑2154636                                                                                                                    |                                                                                                                                 aaCCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTggcgg                                                                                                              <  2:148982/136‑1 (MQ=255) aaCCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTggcgg                                                                                                              >  2:615790/1‑136 (MQ=255)            gggCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTc                                                                                                   <  1:488961/136‑1 (MQ=255)                 cgGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCg                                                                                              <  1:438581/136‑1 (MQ=255)                  ggTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGc                                                                                             >  1:285465/1‑136 (MQ=255)                    tCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCtt                                                                                           >  2:216859/1‑136 (MQ=255)                    tCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCtt                                                                                           >  2:822167/1‑136 (MQ=255)                                 aTTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTg                                                                              >  2:702427/1‑136 (MQ=255)                                 aTTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTg                                                                              >  1:581483/1‑136 (MQ=255)                                     acgcacgcGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAgg                                                                          >  1:743695/1‑136 (MQ=255)                                       gcacgcGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGc                                                                        <  1:372725/136‑1 (MQ=255)                                                    aaCAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGGTTCTTCGATg                                                           <  1:29987/136‑1 (MQ=255)                                                      cAGCGCGCTGGTTCTTCGCCGGGTTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTc                                                         <  2:594068/136‑1 (MQ=255)                                                            gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTc                                                         <  1:178195/130‑1 (MQ=255)                                                            gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTc                                                         >  2:178195/1‑130 (MQ=255)                                                            gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTgtc                                                   >  2:37999/1‑134 (MQ=255)                                                            gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  2:599627/1‑136 (MQ=255)                                                            gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  2:511079/1‑136 (MQ=255)                                                            gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  2:493679/1‑136 (MQ=255)                                                            gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  2:337922/1‑136 (MQ=255)                                                            gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  2:898026/1‑136 (MQ=255)                                                            gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  1:461862/1‑136 (MQ=255)                                                            gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGcc                                                   >  1:48477/1‑136 (MQ=255)                                                            gcTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGAAGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCCCTTCCAGGGAGGACTGCAGCGCGGTTTCTAAGAAGACGCTGcc                                                   >  2:775174/1‑136 (MQ=255)                                                              tGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCtt                                                 >  2:18423/1‑136 (MQ=255)                                                              tGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCtt                                                 >  2:232848/1‑136 (MQ=255)                                                              tGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCtt                                                 >  1:252564/1‑136 (MQ=255)                                                              tGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCACGGCGGTTTCTTCGATGTCGCTGCCtt                                                 >  1:438812/1‑136 (MQ=255)                                                                 ttcttcGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTtgg                                              <  1:24733/136‑1 (MQ=255)                                                                   cttcGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTtggtg                                            <  2:241210/136‑1 (MQ=255)                                                                   cttcGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTtggtg                                            >  2:662351/1‑136 (MQ=255)                                                                        ccGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGgcgcg                                       <  2:227905/136‑1 (MQ=255)                                                                        ccGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTGCTTCGATGTCGCTGCCTTTGGTGgcgcg                                       <  2:562434/136‑1 (MQ=255)                                                                         cGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGgcgcgc                                      >  1:594407/1‑136 (MQ=255)                                                                         cGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGgcgcgc                                      >  1:644506/1‑136 (MQ=255)                                                                                   tCGATGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTgt                            >  2:569411/1‑135 (MQ=255)                                                                                       tGAAGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGtc                        <  1:695162/136‑1 (MQ=255)                                                                                        taaGTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGtcg                       <  1:548044/135‑1 (MQ=255)                                                                                           gTCGATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGtcgata                    >  1:790470/1‑135 (MQ=255)                                                                                              gATGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGac                 <  2:801857/136‑1 (MQ=255)                                                                                               aTGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGacc                >  2:68356/1‑135 (MQ=255)                                                                                                tGACGATCAGGCCGCCGATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGacac               >  2:468757/1‑136 (MQ=255)                                                                                                             gccgATGCCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGACACCAGGGCTtcggtc  <  2:581483/136‑1 (MQ=255)                                                                                                                    |                                                                                                                                 AACCTGCACGCGGGCGCGGTCGGCTTCCAGGCATTCACGCACGCGCTCTTCAACAGCGCGCTGGTTCTTCGCCGGGGTCATGTCGATGAAGTCGATGACGATCAGGCCGCCGATGTCACGCAGGCGCAGCTGGCGGGCGATTTCCTCGGCCGCTTCCAGGTTGGTCTGCAGGGCGGTTTCTTCGATGTCGCTGCCTTTGGTGGCGCGCGCCGAGTTGATGTCGATCGACACCAGGGCTTCGGTC  >  NC_002947/2154393‑2154636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑