BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,293,757	G→A	D350N (GAC→AAC)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,293,757	0	G	A	99.8%	1696.6 / ‑5.7	477	D350N (GAC→AAC)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (243/232); total (243/234)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 477 total aligned reads displayed.

GTCGCCCACGAGTACTTCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAGACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGT  >  NC_002947/2293610‑2293903

gtCGCCCACGAGTACTTCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAAc                                                                                                                                                  <  2:3019523/150‑1 (MQ=255)

     ccACGAGTACTTCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGc                                                                                                                                             >  1:6249420/1‑150 (MQ=255)

     ccACGAGTACTTCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGc                                                                                                                                             <  1:4910224/150‑1 (MQ=255)

       aCGAGTACTTCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCt                                                                                                                                           >  1:4777076/1‑150 (MQ=255)

         gAGTACTTCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTAc                                                                                                                                         <  1:8803958/150‑1 (MQ=255)

         gAGTACTTCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTAc                                                                                                                                         >  1:449676/1‑150 (MQ=255)

         gAGTACTTCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTAc                                                                                                                                         <  1:3394284/150‑1 (MQ=255)

             aCTTCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTgc                                                                                                                                     <  1:3254079/150‑1 (MQ=255)

              cTTCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCTCGATGCCGAGTTCAGTGCAGACATGAACTCGCGCACGGTCAAGCGCAACGAAAACGACGCCTACCTgcg                                                                                                                                    >  2:1579773/1‑150 (MQ=255)

               ttCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTgcgc                                                                                                                                   >  2:5065997/1‑150 (MQ=255)

               ttCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTgcgc                                                                                                                                   >  1:975642/1‑150 (MQ=255)

               ttCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTgcgc                                                                                                                                   >  1:7611201/1‑150 (MQ=255)

               ttCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGAGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTgcgc                                                                                                                                   >  2:9383085/1‑150 (MQ=255)

                     aaCTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGcacccac                                                                                                                             <  1:9045903/150‑1 (MQ=255)

                       cTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCCTCGAAAACGTCGCCTACCTGCGCACccacca                                                                                                                           >  2:9068691/1‑150 (MQ=255)

                       cTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACccacca                                                                                                                           <  1:8447390/150‑1 (MQ=255)

                       cTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACccacca                                                                                                                           <  1:7806458/150‑1 (MQ=255)

                           tCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTc                                                                                                                       <  1:2281647/150‑1 (MQ=255)

                            cGGGCAACCGCGTCACCTGCCGTGACTGGTTCCTGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCg                                                                                                                      <  2:3890240/150‑1 (MQ=255)

                             gggCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAATCGTCGCCTACCTGCGCACCCACCAGTTCGc                                                                                                                     >  2:5519167/1‑150 (MQ=255)

                               gCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCg                                                                                                                   >  1:8083113/1‑150 (MQ=255)

                                 aaCCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGaa                                                                                                                 <  1:3577097/150‑1 (MQ=255)

                                  aCCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAg                                                                                                                >  2:8090311/1‑150 (MQ=255)

                                    cgcgTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCTAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGAc                                                                                                              >  1:2914824/1‑150 (MQ=255)

                                        tCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCgg                                                                                                          <  2:2856647/150‑1 (MQ=255)

                                           ccTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGcc                                                                                                       <  2:4350892/150‑1 (MQ=255)

                                            cTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGAcgc                                                                                                            <  2:4140385/144‑1 (MQ=255)

                                            cTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGccc                                                                                                      >  1:6004521/1‑150 (MQ=255)

                                             tGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGcccc                                                                                                     <  2:7611201/150‑1 (MQ=255)

                                                   gACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGcc                                                                                               >  1:1846775/1‑150 (MQ=255)

                                                   gACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGcc                                                                                               >  1:5149108/1‑150 (MQ=255)

                                                      tGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGTGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACccacca                                                                                                                           >  1:5689600/1‑119 (MQ=255)

                                                      tGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGACGACGCGGGCCCCCTGGCCCAc                                                                                            >  2:2073784/1‑150 (MQ=255)

                                                        gTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCAccc                                                                                          >  1:6492174/1‑150 (MQ=255)

                                                        gTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCAccc                                                                                          >  1:3819145/1‑150 (MQ=255)

                                                          tCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCgg                                                                                        >  1:4677930/1‑150 (MQ=255)

                                                                tGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGc                                                                                                                     >  2:1973845/1‑115 (MQ=255)

                                                                tGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGc                                                                                                                     <  1:1973845/115‑1 (MQ=255)

                                                                tGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGcc                                                                                  <  2:2444430/150‑1 (MQ=255)

                                                                  tCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCg                                                                                <  1:589119/150‑1 (MQ=255)

                                                                    gCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGa                                                                              >  2:8447518/1‑150 (MQ=255)

                                                                    gCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGa                                                                              >  1:1025069/1‑150 (MQ=255)

                                                                         aggaaggCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCt                                                                         <  2:2213724/150‑1 (MQ=255)

                                                                          ggaaggCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCtt                                                                        >  2:6455576/1‑150 (MQ=255)

                                                                          ggaaggCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCtt                                                                        >  1:4188946/1‑150 (MQ=255)

                                                                           gaaggCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTc                                                                       <  1:4439840/150‑1 (MQ=255)

                                                                           ctcggCATCGCGGAATACCGTGAAGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTc                                                                       >  1:7275052/4‑150 (MQ=255)

                                                                            aaggCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGaa                                                                                                                 <  2:4743967/107‑1 (MQ=255)

                                                                            aaggCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCa                                                                      >  1:2761345/1‑150 (MQ=255)

                                                                             aggCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCAt                                                                     <  2:7758324/150‑1 (MQ=255)

                                                                             aggCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCAt                                                                     >  2:6905649/1‑150 (MQ=255)

                                                                                 ttCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGaa                                                                 >  2:5957922/1‑150 (MQ=255)

                                                                                 ttCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGaa                                                                 >  2:8613816/1‑150 (MQ=255)

                                                                                  tCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGaaa                                                                <  1:2211193/150‑1 (MQ=255)

                                                                                   cACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAAt                                                               >  1:6945307/1‑150 (MQ=255)

                                                                                      ggTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCg                                                                                                                      >  2:6274853/1‑92 (MQ=255)

                                                                                      ggTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAAtctc                                                            <  2:2362242/150‑1 (MQ=255)

                                                                                        tATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCa                                                          >  1:7195557/1‑150 (MQ=255)

                                                                                             cgcgATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTc                                                     >  2:7973438/1‑150 (MQ=255)

                                                                                              gcgATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGaa                                                                                                                 <  2:5258212/89‑1 (MQ=255)

                                                                                              gcgATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCt                                                    <  1:8711666/150‑1 (MQ=255)

                                                                                                gATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTac                                                  <  1:3868008/150‑1 (MQ=255)

                                                                                                   gCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACAcc                                               <  1:5851367/150‑1 (MQ=255)

                                                                                                     cGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCggg                                                                                                         <  2:4454516/90‑1 (MQ=255)

                                                                                                     cGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCt                                             <  2:4010668/150‑1 (MQ=255)

                                                                                                      gAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTg                                            >  1:9421578/1‑150 (MQ=255)

                                                                                                      gAGTTCAGTGCCGACAGGAACTCGCGCACGGTCAAGCGCAGCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCAGGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTg                                            >  1:794178/1‑150 (MQ=255)

                                                                                                       aGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACTCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGa                                           <  1:2331072/150‑1 (MQ=255)

                                                                                                         ttCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGAcc                                         >  1:6348738/1‑150 (MQ=255)

                                                                                                         ttCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGAcc                                         >  2:672091/1‑150 (MQ=255)

                                                                                                          tCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCGACTTCTACACCCTGACCg                                        >  1:8630533/1‑150 (MQ=255)

                                                                                                            aGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGt                                                                                       >  1:4987614/1‑101 (MQ=255)

                                                                                                            aGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGt                                                                                       <  2:4987614/101‑1 (MQ=255)

                                                                                                            aGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTc                                      >  2:9238714/1‑150 (MQ=255)

                                                                                                             gTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAATCGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCt                                     >  2:6361306/1‑150 (MQ=255)

                                                                                                              tGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTa                                    <  2:1203850/150‑1 (MQ=255)

                                                                                                                 cGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCCCCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACga                                 <  1:2073784/150‑1 (MQ=255)

                                                                                                                 cGACATGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATc                                                                    <  1:1439306/115‑1 (MQ=255)

                                                                                                                      tGAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAgg                            >  1:2624628/1‑150 (MQ=255)

                                                                                                                       gAACTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCAGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAggg                           >  1:6812007/1‑150 (MQ=255)

                                                                                                                        aaCTCGCGCAAGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACAAGTTCGCCGAAGACGCGGGCCCAATGGCCCACACTGTGCGCCCGCACAGCTTCATCGAAATATCCAACATCTACACCCTGACAGACTACTAGAAGGgc                          >  2:343334/1‑150 (MQ=255)

                                                                                                                          cTCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGgcgc                        <  2:1448512/150‑1 (MQ=255)

                                                                                                                           tCGCGCACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGcc                       >  1:1216457/1‑150 (MQ=255)

                                                                                                                             gcgcACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGGCTACGTGAAGGGCGCCGa                     >  1:5564581/1‑150 (MQ=255)

                                                                                                                                cACGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAgt                  >  2:1834851/1‑150 (MQ=255)

                                                                                                                                  cGGTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAgtgg                <  2:4812499/150‑1 (MQ=255)

                                                                                                                                   ggTCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAgtggt               >  2:8973111/1‑150 (MQ=255)

                                                                                                                                     tCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTcc                                                           <  2:3823401/104‑1 (MQ=255)

                                                                                                                                     tCAAGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTgc             <  1:6617234/150‑1 (MQ=255)

                                                                                                                                       aaGCGCATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTgcgc           >  2:8355769/1‑150 (MQ=255)

                                                                                                                                         gcgcATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGTCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCaa                                                         <  2:6223013/102‑1 (MQ=255)

                                                                                                                                          cgcATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATg        <  2:2624628/150‑1 (MQ=255)

                                                                                                                                           gcATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTgc             <  1:4075057/144‑1 (MQ=255)

                                                                                                                                           gcATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATgg       <  2:3334982/150‑1 (MQ=255)

                                                                                                                                            cATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGt      <  2:1025069/150‑1 (MQ=255)

                                                                                                                                            cATCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGt      >  1:4305904/1‑150 (MQ=255)

                                                                                                                                             aTCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTc     <  2:7275122/150‑1 (MQ=255)

                                                                                                                                             aTCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTc     <  2:292823/150‑1 (MQ=255)

                                                                                                                                             aTCGAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTc     >  1:7438407/1‑150 (MQ=255)

                                                                                                                                                gAAAACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGt  >  1:1610891/1‑150 (MQ=255)

GTCGCCCACGAGTACTTCCACAACTGGTCGGGCAACCGCGTCACCTGCCGTGACTGGTTCCAGCTGTCGCTGAAGGAAGGCTTCACGGTATTCCGCGATGCCGAGTTCAGTGCCGACATGAACTCGCGCACGGTCAAGCGCATCGAAGACGTCGCCTACCTGCGCACCCACCAGTTCGCCGAAGACGCGGGCCCCATGGCCCACCCGGTGCGCCCGGACAGCTTCATCGAAATCTCCAACTTCTACACCCTGACCGTCTACGAGAAGGGCGCCGAAGTGGTGCGCATGGTCCGT  >  NC_002947/2293610‑2293903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`