Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,542,538:3 +G 17.2% coding (880/1533 nt) PP_4025 → transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,542,5383.G17.2% 175.1 / 42.2 58coding (880/1533 nt)PP_4025transposase
Reads supporting (aligned to +/- strand):  ref base . (28/20);  new base G (8/2);  total (36/22)
Fisher's exact test for biased strand distribution p-value = 2.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

ATGAAACTCGCGTGCAGGTGCTCAAAGAGCCGGATCGTGATCCGACCAGTCAATCCTGGATGTGGGTGCAGGCCAGTGGCCCGCCTGATCGACAAGTCGTGCTGTTCGATTACACCACCAGCCGCGCGCAGGAGGTGCCGCTGCGCCTGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAAGAGC  >  NC_002947/4542389‑4542687
                                                                                                                                                        |                                                                                                                                                     
cttcgacgaatttacgccggacctgcgccatgcacgccagtcgatcaacgcccggctgtagctccaaagcgttgtaacaggcgaaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGc                                                                                                                                                          >  2:474387‑M2/149‑150 (MQ=255)
cttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccggcaggcgcagcggcacctcctgcgcgcgGc                                                                                                                                                          >  2:5628440‑M2/149‑150 (MQ=255)
cttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGc                                                                                                                                                          <  1:122394‑M2/2‑1 (MQ=255)
cttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGc                                                                                                                                                          >  1:1445797‑M2/149‑150 (MQ=255)
cttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGc                                                                                                                                                          <  1:2933086‑M2/2‑1 (MQ=255)
 ttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑t                                                                                                                                                      <  2:3680199‑M2/3‑1 (MQ=255)
 ttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑t                                                                                                                                                      >  1:497370‑M2/148‑150 (MQ=255)
 ttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑t                                                                                                                                                      <  2:2882400‑M2/3‑1 (MQ=255)
 ttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑t                                                                                                                                                      >  1:2874840‑M2/148‑150 (MQ=255)
 ttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑t                                                                                                                                                      <  2:3680214‑M2/3‑1 (MQ=255)
 ttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑t                                                                                                                                                      >  2:905275‑M2/148‑150 (MQ=255)
 ttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑t                                                                                                                                                      >  2:477113‑M2/148‑150 (MQ=255)
 ttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑t                                                                                                                                                      <  2:71094‑M2/3‑1 (MQ=255)
 ttcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑t                                                                                                                                                      >  1:1615723‑M2/148‑150 (MQ=255)
  tcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatctcgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tg                                                                                                                                                     <  1:962262‑M2/4‑1 (MQ=255)
  tcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tg                                                                                                                                                     <  2:1350674‑M2/4‑1 (MQ=255)
  tcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tg                                                                                                                                                     <  1:482035‑M2/4‑1 (MQ=255)
  tcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tg                                                                                                                                                     <  2:1350406‑M2/4‑1 (MQ=255)
  tcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tg                                                                                                                                                     >  1:1915858‑M2/147‑150 (MQ=255)
  tcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tg                                                                                                                                                     <  1:1570016‑M2/4‑1 (MQ=255)
  tcgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tg                                                                                                                                                     <  1:1570139‑M2/4‑1 (MQ=255)
   cgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tgg                                                                                                                                                    <  1:3102485‑M2/5‑1 (MQ=255)
   cgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tgg                                                                                                                                                    <  1:3102528‑M2/5‑1 (MQ=255)
   cgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tgg                                                                                                                                                    <  1:3102652‑M2/5‑1 (MQ=255)
   cgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tgg                                                                                                                                                    <  1:3376956‑M2/5‑1 (MQ=255)
   cgacgaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑Tgg                                                                                                                                                    >  1:1954432‑M2/146‑150 (MQ=255)
       gaatttacgccggacatgcgccatgcacgccagtcgttcaacgcccggctgtagcgccaacgcgttgtaaccggcgtaatcatcggtcatcacgtaaccgcgatagccctccagcaggcgcagcggcacctcctgcgcgcggc‑‑‑tgggccc                                                                                                                                                >  1:5604303‑M2/142‑146 (MQ=255)
                                                                                                         cgcgatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGTGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGc                                              >  1:4280573‑M2/44‑150 (MQ=255)
                                                                                                            gtaaccgcgatagccctccagcaggcgcagcggcacctccG‑‑‑‑‑GGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCCTGGCGCATGTCCGGCGtaac                                         >  1:2433927‑M2/41‑149 (MQ=255)
                                                                                                            gatagccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGTGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTTCGGCGTa                                           >  2:2992287‑M2/41‑150 (MQ=255)
                                                                                                             gtaaccgcgatagccctccagcaggcgcagcggcacctc‑C‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTaaa                                         >  1:656736‑M2/40‑150 (MQ=255)
                                                                                                                 ccctccagcaggcgcagcggcacctcctgcgcgcgGT‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTTCGGCGTAAATtc                                      >  2:1574661‑M2/36‑150 (MQ=255)
                                                                                                                 ccctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATtc                                      >  1:3690604‑M2/36‑150 (MQ=255)
                                                                                                                  cctccagcaggcgcagcggcacctcctgcgcgcgGCTGGTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTg                                                               >  2:718251‑M2/35‑127 (MQ=255)
                                                                                                                  cctccagcaggcgcagcggcacctcctgcgcgcgGCTGGTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTg                                                               <  1:718251‑M2/93‑1 (MQ=255)
                                                                                                                  cctccagcaggcgcagcggcacctcctgcgcgcgGCTGGTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAAt                                        >  2:1085665‑M2/35‑150 (MQ=255)
                                                                                                                  cctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTTCGGCGTAAATtcg                                     >  2:4546455‑M2/35‑150 (MQ=255)
                                                                                                                  cctccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATtcg                                     >  2:2608008‑M2/35‑150 (MQ=255)
                                                                                                                   ctccagcaggcgcagcggcacctcctgcgcgcgGCTGGTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGa                                                                           <  2:3033323‑M2/81‑1 (MQ=255)
                                                                                                                   ctccagcaggcgcagcggcacctcctgcgcgcgGCTGGTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGa                                                                           >  1:3033323‑M2/34‑114 (MQ=255)
                                                                                                                   ctccagcaggcgcagcggcacctcctgcgcgcgGCTGGTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCACTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAAtt                                       >  1:1805400‑M2/34‑150 (MQ=255)
                                                                                                                    tccagcaggcgcagcggcacctcctgcgcgcgGCTGGTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTTCGGCGTAAATtc                                      >  2:447903‑M2/33‑150 (MQ=255)
                                                                                                                    tccagcaggcgcagcggcacctcctgcgcgcgGCTGGTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATtc                                      >  2:3782706‑M2/33‑150 (MQ=255)
                                                                                                                    tccagcaggcgcagcggcacctcctgcgcgcgGCTGGTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATtc                                      >  2:5514486‑M2/33‑150 (MQ=255)
                                                                                                                    tccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCGTGTCCGGCGTAAATtcgtc                                   >  2:1698388‑M2/33‑150 (MQ=255)
                                                                                                                    tccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTTCGGCGTAAATtcgtc                                   >  1:1149647‑M2/33‑150 (MQ=255)
                                                                                                                    tccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTTCGGCGTAAATtcgtc                                   >  1:2561240‑M2/33‑150 (MQ=255)
                                                                                                                    tccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATtcgtc                                   >  2:1698387‑M2/33‑150 (MQ=255)
                                                                                                                    tccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATtcgtc                                   >  2:2220901‑M2/33‑150 (MQ=255)
                                                                                                                    tccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATtcgtc                                   >  2:4892482‑M2/33‑150 (MQ=255)
                                                                                                                    tccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATtcgtc                                   >  1:5629540‑M2/33‑150 (MQ=255)
                                                                                                                    tccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATtcgtc                                   >  1:2811253‑M2/33‑150 (MQ=255)
                                                                                                                     ccagcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATtcgtcg                                  >  1:586775‑M2/32‑150 (MQ=255)
                                                                                                                      ccagcaggcgcagcggcacctcctgcgcctGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGa                                 >  1:2388966‑M2/31‑150 (MQ=255)
                                                                                                                      cagcaggcgcagcggcacctcctgcgcgcgGCTGGTGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGTGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATtcgt                                    >  2:494864‑M2/31‑150 (MQ=255)
                                                                                                                        ggcgcagcggcacctcctgcgcgcggctGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGACGCATGTCCGGCGTAAATTCGTCGAAg                               >  1:281815‑M2/29‑150 (MQ=255)
                                                                                                                        gcaggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAg                               >  1:5588622‑M2/29‑150 (MQ=255)
                                                                                                                         gcaggcgcagcggcacctcctgcgcctGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAgc                              >  2:3996772‑M2/28‑150 (MQ=255)
                                                                                                                         gcaggcgcagcggcacctcctgcgcctGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAgc                              >  1:5447244‑M2/28‑150 (MQ=255)
                                                                                                                           ggcgcagcggcacctcctgcgcgcgGC‑‑‑TGGAGGGCTAGCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTTCGGCGTAAATTCGTCGAAgcgc                            >  1:987736‑M2/26‑150 (MQ=255)
                                                                                                                            ggcgcagcggcacctcctgcgcctGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTTCGGCGTAAATTCGTCGAAGCGCa                           >  1:1050005‑M2/25‑150 (MQ=255)
                                                                                                                             agcggcacctcctgcgcgcgcctGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAg                          >  2:299612‑M2/24‑150 (MQ=255)
                                                                                                                                                    GC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAagag   <  2:2253892‑M2/150‑1 (MQ=255)
                                                                                                                                                    GC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAagag   >  2:645672‑M2/1‑150 (MQ=255)
                                                                                                                                                     C‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAgggcaacatc  <  1:471800‑M2/150‑5 (MQ=255)
                                                                                                                                                     C‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAgggcaacatc  <  1:779635‑M2/150‑5 (MQ=255)
                                                                                                                                                     C‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAgggcaacatc  <  1:471893‑M2/150‑5 (MQ=255)
                                                                                                                                                     C‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAAGAGc  <  1:2951720‑M2/150‑1 (MQ=255)
                                                                                                                                                     C‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAAGAGc  <  2:4339385‑M2/150‑1 (MQ=255)
                                                                                                                                                     C‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAAGAGc  <  2:1453616‑M2/150‑1 (MQ=255)
                                                                                                                                                     C‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAAGAGc  <  1:704467‑M2/150‑1 (MQ=255)
                                                                                                                                                     C‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAAGAGc  <  1:1694664‑M2/150‑1 (MQ=255)
                                                                                                                                                     C‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAAGAGc  <  1:1693812‑M2/150‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
ATGAAACTCGCGTGCAGGTGCTCAAAGAGCCGGATCGTGATCCGACCAGTCAATCCTGGATGTGGGTGCAGGCCAGTGGCCCGCCTGATCGACAAGTCGTGCTGTTCGATTACACCACCAGCCGCGCGCAGGAGGTGCCGCTGCGCCTGC‑‑‑TGGAGGGCTATCGCGGTTACGTGATGACCGATGATTACGCCGGTTACAACGCGTTGGCGCTACAGCCGGGCGTTGAACGACTGGCGTGCATGGCGCATGTCCGGCGTAAATTCGTCGAAGCGCAGAAGGTGCAGCCTAAGGGCAAGAGC  >  NC_002947/4542389‑4542687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑