BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,345,571:1	+A	5.4%	coding (249/1587 nt)	ybiT ←	ABC‑F family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,345,571	1	.	A	5.4%	1214.1 / 58.9	279	coding (249/1587 nt)	ybiT	ABC‑F family ATPase
Reads supporting (aligned to +/- strand): ref base . (136/128); new base A (3/12); total (139/140)
Fisher's exact test for biased strand distribution p-value = 3.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 284 total aligned reads displayed.

ACTCGGCGGTGTAGCCGTCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTCGACTTGCCGCAACCGTTGGCGCC  >  NC_002947/3345423‑3345720

 cTCGGCGGTGTAGCCGTCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑Tc                                                                                                                                                     <  2:2688623/150‑1 (MQ=255)

  tCGGCGGTGTAGCCGTCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCg                                                                                                                                                    >  1:582343/1‑150 (MQ=255)

           tAGCCGTCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTg                                                                                                                                           >  2:4624107/1‑150 (MQ=255)

           gaGCCGTCCATGTCGGCGAATTCGGGTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTg                                                                                                                                           <  2:579152/149‑1 (MQ=255)

            aGCCGTCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCCCAGCTGGCCGTGGCCCATGATCACCGTGATCGA‑CACGGTGa                                                                                                                                          <  2:4827313/150‑1 (MQ=255)

            aGCCGTCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTGATCGA‑CACGGTGa                                                                                                                                          <  2:3575293/150‑1 (MQ=255)

            aGCCGTCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTGATCGA‑CACGGTGa                                                                                                                                          <  1:1773190/150‑1 (MQ=255)

               cGTCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTGATCGA‑CACGGTGatca                                                                                                                                       <  2:1924687/150‑4 (MQ=255)

                gTCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCGCCGTG‑TCGATCACGGTGAATTc                                                                                                                                      >  2:2024261/1‑150 (MQ=255)

                 tCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTcc                                                                                                                                     >  1:5165516/1‑150 (MQ=255)

                 tCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTcc                                                                                                                                     >  1:5108384/1‑150 (MQ=255)

                 tCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTcc                                                                                                                                     >  1:3071280/1‑150 (MQ=255)

                  ccATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTGATCGA‑CACGGTGatcatgg                                                                                                                                    <  1:4222524/150‑7 (MQ=255)

                   cATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTc                                                                                                                                   <  2:4711043/150‑1 (MQ=255)

                      ttCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTa                                                                                                                                <  1:2352150/150‑1 (MQ=255)

                       tCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAg                                                                                                                               <  1:4936814/150‑1 (MQ=255)

                        cGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAgg                                                                                                                              <  1:1681643/150‑1 (MQ=255)

                            gAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGaa                                                                                                                          >  2:5404369/1‑150 (MQ=255)

                               ttCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTg                                                                                                                       <  2:5060330/150‑1 (MQ=255)

                               ttCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTg                                                                                                                       >  2:132949/1‑150 (MQ=255)

                               ttCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTg                                                                                                                       >  2:2925990/1‑150 (MQ=255)

                               ttCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTg                                                                                                                       >  2:2581386/1‑150 (MQ=255)

                                 cGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTa                                                                                                                                >  1:632987/1‑139 (MQ=255)

                                 cGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGt                                                                                                                     >  2:1240851/1‑150 (MQ=255)

                                  ggTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTc                                                                                                                    >  2:1499268/1‑150 (MQ=255)

                                    aaacgaGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCt                                                                                                                  <  1:3162268/145‑1 (MQ=255)

                                         aGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGgcgc                                                                                                             <  2:3443459/150‑1 (MQ=255)

                                         aGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGgcgc                                                                                                             >  1:2143069/1‑150 (MQ=255)

                                         aGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGgcgc                                                                                                             >  1:4889383/1‑150 (MQ=255)

                                          gCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCa                                                                                                            <  1:3018575/150‑1 (MQ=255)

                                           cTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAg                                                                                                           >  1:4362132/1‑150 (MQ=255)

                                            tCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGc                                                                                                          <  1:554767/150‑1 (MQ=255)

                                              ggCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCtt                                                                                                        <  2:5015683/150‑1 (MQ=255)

                                                cGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGc                                                                                                      <  2:554608/150‑1 (MQ=255)

                                                 gACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGcc                                                                                                     >  2:4243301/1‑150 (MQ=255)

                                                    ggCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCAATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAg                                                                                                  >  2:3921417/1‑150 (MQ=255)

                                                      ccATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGc                                                                                                >  1:2607045/1‑150 (MQ=255)

                                                      ccATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGc                                                                                                <  2:3289491/150‑1 (MQ=255)

                                                       cATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCg                                                                                               >  1:136635/1‑150 (MQ=255)

                                                          gCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGt                                                                                            >  1:4045165/1‑150 (MQ=255)

                                                           ccGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTa                                                                                           <  2:3778448/150‑1 (MQ=255)

                                                              tcttctTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTc                                                                                        >  2:4494552/1‑150 (MQ=255)

                                                                ttcttcGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCgg                                                                                      >  2:1418381/1‑150 (MQ=255)

                                                                   ttcGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTc                                                                                   <  1:1002588/150‑1 (MQ=255)

                                                                   ctcGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTc                                                                                   <  1:35749/149‑1 (MQ=255)

                                                                   ctcGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTc                                                                                   <  1:36295/149‑1 (MQ=255)

                                                                     cGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGa                                                                                 >  1:4673573/1‑150 (MQ=255)

                                                                      ggTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAg                                                                                >  2:93196/1‑150 (MQ=255)

                                                                      ggTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAg                                                                                >  2:610338/1‑150 (MQ=255)

                                                                       gTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGc                                                                               >  1:3888761/1‑150 (MQ=255)

                                                                       gTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGc                                                                               >  2:4455101/1‑150 (MQ=255)

                                                                         cATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCAt                                                                             >  2:2304503/1‑150 (MQ=255)

                                                                           tttCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGa                                                                           >  2:524536/1‑150 (MQ=255)

                                                                           tttCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGa                                                                           >  1:1830136/1‑150 (MQ=255)

                                                                            gtCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGGGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGAc                                                                          <  2:5198780/149‑1 (MQ=255)

                                                                                 gCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGc                                                                     <  2:3901507/150‑1 (MQ=255)

                                                                                 gCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGc                                                                     <  2:3901995/150‑1 (MQ=255)

                                                                                 gCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGc                                                                     <  2:1801336/150‑1 (MQ=255)

                                                                                 gCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGc                                                                     <  2:4343164/150‑1 (MQ=255)

                                                                                 gCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGc                                                                     >  2:2648519/1‑150 (MQ=255)

                                                                                      gAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCg                                                                >  2:3035231/1‑150 (MQ=255)

                                                                                      gAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCg                                                                >  2:309/1‑150 (MQ=255)

                                                                                      gAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCg                                                                >  1:4286970/1‑150 (MQ=255)

                                                                                      gAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCg                                                                <  1:4479291/150‑1 (MQ=255)

                                                                                      gAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCg                                                                >  1:924794/1‑150 (MQ=255)

                                                                                        gTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGa                                                              <  1:84222/150‑1 (MQ=255)

                                                                                         tAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGAc                                                             <  2:3139069/150‑1 (MQ=255)

                                                                                           gATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACgg                                                           >  2:656181/1‑150 (MQ=255)

                                                                                                 gTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAg                                                     <  1:3790113/150‑1 (MQ=255)

                                                                                                     cGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCa                                                 >  1:1121368/1‑150 (MQ=255)

                                                                                                        tCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCAccg                                              >  1:3258531/1‑150 (MQ=255)

                                                                                                         cGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCAccgc                                             <  2:2438316/150‑1 (MQ=255)

                                                                                                          ggCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCATGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCAccgcc                                            >  1:5036229/1‑150 (MQ=255)

                                                                                                            cTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGa                                          <  2:2797311/150‑1 (MQ=255)

                                                                                                             tttGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAg                                         >  1:2506362/1‑150 (MQ=255)

                                                                                                               tGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGa                                       <  2:2235306/150‑1 (MQ=255)

                                                                                                               tGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGa                                       <  1:2372223/150‑1 (MQ=255)

                                                                                                                   tttCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGAtttt                                   >  1:1207835/1‑150 (MQ=255)

                                                                                                                   tttCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGAtttt                                   >  1:310390/1‑150 (MQ=255)

                                                                                                                   tttCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTAGTTGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGAtttt                                   >  2:353948/1‑150 (MQ=255)

                                                                                                                     tCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCa                                 <  2:5108384/150‑1 (MQ=255)

                                                                                                                      ccACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCAt                                >  2:3674299/1‑150 (MQ=255)

                                                                                                                       cacaGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATg                               >  2:3376941/1‑150 (MQ=255)

                                                                                                                        acaGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGa                              >  1:2244939/1‑150 (MQ=255)

                                                                                                                          aGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAg                            <  1:1058550/150‑1 (MQ=255)

                                                                                                                          aGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAg                            >  2:3223440/1‑150 (MQ=255)

                                                                                                                          aGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAg                            >  2:4889507/1‑150 (MQ=255)

                                                                                                                           gCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAgg                           >  2:757193/1‑150 (MQ=255)

                                                                                                                             tGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTc                         >  2:3194096/1‑150 (MQ=255)

                                                                                                                             tGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTc                         >  2:1088620/1‑150 (MQ=255)

                                                                                                                                    ggCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACAGCCGAGGATTTTCATGAAGGTCGACTTGc                  <  2:825654/150‑1 (MQ=255)

                                                                                                                                       ccATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTCGACTTGCCGc               >  1:1571749/1‑150 (MQ=255)

                                                                                                                                         aTGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTCGACTTGCCGCaa             <  1:4095342/150‑1 (MQ=255)

                                                                                                                                           gATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTCGACTTGCCGCAAcc           >  2:2314695/1‑150 (MQ=255)

                                                                                                                                            ttCACCGTGATCGA‑CACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAGGGTCGACTTGCCGCAACCg          >  1:3877396/2‑150 (MQ=255)

                                                                                                                                             tCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTCGACTTGCCGCAACCGt         >  1:1096476/1‑150 (MQ=255)

                                                                                                                                              cACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTCGACTTGCCGCAACCGtt        <  2:4124092/150‑1 (MQ=255)

                                                                                                                                              cACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTCGACTTGCCGCAACCGtt        >  2:4069222/1‑150 (MQ=255)

                                                                                                                                               aCCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTCGACTTGCCGCAACCGTTg       <  2:1147369/150‑1 (MQ=255)

                                                                                                                                                 cGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTCGACTTGCCGCAACCGTTGgc     <  1:2713066/150‑1 (MQ=255)

ACTCGGCGGTGTAGCCGTCCATTTCGGCGAATTCGGTTTCCAGCTCGGCGACGGCCATGCCGTCTTCTTCGGTCATTTCCGGCAGCGAGTAGATACGGTCACGCTCGGCTTTGACTTTCCACAGCTGGCCGTGGCCCATGATCACCGTG‑TCGATCACGGTGAATTCCTCGTAGGCGAACTGGTCCTGGCGCAGCTTGCCCAGGCGGGTATTCGGCTCGAGCATGACCTGGCCTGCGGACGGCTCCAGGTCACCGCCGAGGATTTTCATGAAGGTCGACTTGCCGCAACCGTTGGCGCC  >  NC_002947/3345423‑3345720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`