Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,143,578 C→A 5.9% G28G (GGC→GGA)  PP_3643 → oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,143,5780CA 5.9% 732.6 / 26.1 219G28G (GGC→GGA) PP_3643oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (99/107);  new base A (3/10);  total (102/117)
Fisher's exact test for biased strand distribution p-value = 9.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

Only 100 of 230 total aligned reads displayed.

GGTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGCA  >  NC_002947/4143429‑4143727
                                                                                                                                                     |                                                                                                                                                     
ggTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGc                                                                                                                                                       <  2:1236759/150‑1 (MQ=255)
ggTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGc                                                                                                                                                       <  2:4685005/150‑1 (MQ=255)
ggTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGc                                                                                                                                                       <  2:1714640/150‑1 (MQ=255)
ggTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGc                                                                                                                                                       >  1:153469/1‑150 (MQ=255)
  tataGCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTc                                                                                                                                                     >  2:3258859/1‑150 (MQ=255)
   ataCCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCg                                                                                                                                                    <  1:4745362/150‑1 (MQ=255)
   ataCCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCg                                                                                                                                                    <  2:2583274/150‑1 (MQ=255)
    taCCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCgg                                                                                                                                                   >  2:1817834/1‑150 (MQ=255)
       cATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGgtcg                                                                                                                                                >  1:5103809/1‑150 (MQ=255)
        aTCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGgtcgt                                                                                                                                               >  2:3855469/1‑150 (MQ=255)
         tCAGACACATCAACCATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGgtcgtc                                                                                                                                              <  2:2247102/150‑1 (MQ=255)
           aGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCAGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCgg                                                                                                                                            >  1:5275007/1‑150 (MQ=255)
            gACACATCAACTATAAAAACCGCGGACCACCGCAGCAATTCAGAGGTAATCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTAACGTCATCACCACACAGGACGACGACGGCTCGGTCGTCGGc                                                                                                                                           >  1:1688767/1‑150 (MQ=255)
            gACACATCAACGATAACAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACCGAGCCGtcgtcgtc                                                                                                                                           <  2:5568847/150‑3 (MQ=255)
            gACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGc                                                                                                                                           <  2:1148543/150‑1 (MQ=255)
             acacATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGTCGTcc                                                                                                                                          <  2:5427490/150‑1 (MQ=255)
             acacATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGcc                                                                                                                                          <  1:853472/150‑1 (MQ=255)
              cacaTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGTCGTCCt                                                                                                                                         <  2:3681830/150‑1 (MQ=255)
              cacaTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACCGAGCCGTCGTCGTCCt                                                                                                                                         <  2:575425/150‑1 (MQ=255)
                caTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGa                                                                                                                                       <  1:3672531/150‑1 (MQ=255)
                 aTCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGAc                                                                                                                                      >  2:2938432/1‑150 (MQ=255)
                    aaCTATAAAAACCGCGGACCACCGCAGCCCTTCAGAGTTACTCCCTATGATCGACGAAAATGTATAAAATAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTAATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGc                                                                                                                                   <  1:724420/150‑1 (MQ=255)
                    aaCGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGc                                                                                                                                   >  2:4047/1‑150 (MQ=255)
                      cGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAATAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCa                                                                                                                                 >  1:494127/1‑150 (MQ=255)
                      cGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCa                                                                                                                                 >  1:3308089/1‑150 (MQ=255)
                        aTAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAgc                                                                                                                               <  2:1053885/150‑1 (MQ=255)
                                cgcgGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTtctc                                                                                                                       <  1:2205340/150‑1 (MQ=255)
                                cgcgGACCACCGAAGCCCTTCAGAGTTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCGGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTtctc                                                                                                                       <  2:1884857/150‑1 (MQ=255)
                                 gcgGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCAGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTctcc                                                                                                                      <  2:5274775/150‑1 (MQ=255)
                                   ggACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTctcctc                                                                                                                    <  1:5611568/150‑1 (MQ=255)
                                   ggACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGAAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTctcctc                                                                                                                    <  1:2345681/150‑1 (MQ=255)
                                           gCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGa                                                                                                            <  1:5390277/150‑1 (MQ=255)
                                            cAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGAt                                                                                                           <  2:625763/150‑1 (MQ=255)
                                            cAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGAt                                                                                                           <  2:2639316/150‑1 (MQ=255)
                                            cAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGAt                                                                                                           <  2:3437513/150‑1 (MQ=255)
                                             aGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGTGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATg                                                                                                          >  2:1312117/1‑150 (MQ=255)
                                               cccTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGa                                                                                                        <  2:3066234/150‑1 (MQ=255)
                                                ccTTCAGAGGTACTCCCGCTGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGAc                                                                                                       <  2:3313553/150‑1 (MQ=255)
                                                  ttCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGAccc                                                                                                     >  1:3247887/1‑150 (MQ=255)
                                                  ttCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGAccc                                                                                                     >  2:4414574/1‑150 (MQ=255)
                                                  gtCAGAGGTACTCCCGATGATCGACGCAACTGTCTACACGACCGTTCGGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGCCGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGAccc                                                                                                     <  1:1042905/149‑1 (MQ=255)
                                                          tACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTgg                                                                                             >  1:4110696/1‑150 (MQ=255)
                                                            cTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTg                                                                                           <  1:920443/150‑1 (MQ=255)
                                                            cTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTg                                                                                           <  1:17057/150‑1 (MQ=255)
                                                                     atCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGc                                                                                  >  2:4275938/1‑150 (MQ=255)
                                                                      tCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGcc                                                                                 <  2:4837132/150‑1 (MQ=255)
                                                                       cGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGccc                                                                                <  1:3013763/150‑1 (MQ=255)
                                                                       cGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGccc                                                                                >  2:1039123/1‑150 (MQ=255)
                                                                        gACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGcccc                                                                               >  2:4773516/1‑150 (MQ=255)
                                                                        gACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGcccc                                                                               <  1:2664291/150‑1 (MQ=255)
                                                                          cGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCaa                                                                             <  2:1790179/150‑1 (MQ=255)
                                                                           gCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAAc                                                                            <  1:3335572/150‑1 (MQ=255)
                                                                             aaCTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTa                                                                          >  2:2024472/1‑150 (MQ=255)
                                                                              aCTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTac                                                                         <  1:600103/150‑1 (MQ=255)
                                                                                  tCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCt                                                                     >  1:3970200/1‑150 (MQ=255)
                                                                                    tACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTcc                                                                   <  2:2736201/150‑1 (MQ=255)
                                                                                    tACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTcc                                                                   >  1:942810/1‑150 (MQ=255)
                                                                                    tACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTcc                                                                   >  2:172614/1‑150 (MQ=255)
                                                                                    tACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTcc                                                                   >  2:4307226/1‑150 (MQ=255)
                                                                                       aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGAc                                                                >  2:1518986/1‑150 (MQ=255)
                                                                                       aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGAc                                                                >  2:548952/1‑150 (MQ=255)
                                                                                       aaGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGAc                                                                >  1:3570540/1‑150 (MQ=255)
                                                                                            cGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTa                                                           <  2:3381022/150‑1 (MQ=255)
                                                                                                 tGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTCCCCgg                                                      >  1:4796277/1‑150 (MQ=255)
                                                                                                   ggCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTa                                                    >  2:4936913/1‑150 (MQ=255)
                                                                                                   ggCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTa                                                    <  2:821521/150‑1 (MQ=255)
                                                                                                   ggCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTa                                                    <  1:272991/150‑1 (MQ=255)
                                                                                                       ccTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGa                                                <  2:2688049/150‑1 (MQ=255)
                                                                                                       ccTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGa                                                <  1:4235942/150‑1 (MQ=255)
                                                                                                        cTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGAt                                               <  1:3777372/150‑1 (MQ=255)
                                                                                                         ttCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATc                                              >  2:2696703/1‑150 (MQ=255)
                                                                                                         ttCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATc                                              <  2:1691441/150‑1 (MQ=255)
                                                                                                         ttCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATc                                              <  2:4871216/150‑1 (MQ=255)
                                                                                                         ttCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATc                                              >  1:3404548/1‑150 (MQ=255)
                                                                                                           cccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCaa                                            <  2:785456/150‑1 (MQ=255)
                                                                                                           cccGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCaa                                            <  2:3975196/150‑1 (MQ=255)
                                                                                                             cGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGc                                          >  1:21190/1‑150 (MQ=255)
                                                                                                             cGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGc                                          >  2:1602162/1‑150 (MQ=255)
                                                                                                             cGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGc                                          >  1:281906/1‑150 (MQ=255)
                                                                                                              gTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCa                                         >  2:4332879/1‑150 (MQ=255)
                                                                                                                  ggCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAg                                     >  1:4509393/1‑150 (MQ=255)
                                                                                                                  ggCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGAGCTGTTCTGCCCCAACTACACCTCCGACTCCAACCCGGTACTGATCAAGCAGAAg                                     >  2:4422782/1‑150 (MQ=255)
                                                                                                                      ttACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTCCTGATCAAGCAGAAGCGCt                                 >  2:4071180/1‑150 (MQ=255)
                                                                                                                        aCCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCttt                               <  1:1313266/150‑1 (MQ=255)
                                                                                                                        aCCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCttt                               >  2:5307069/1‑150 (MQ=255)
                                                                                                                                caccacCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCa                       <  1:5306267/150‑1 (MQ=255)
                                                                                                                                  ccaccCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGa                                                                 >  2:4266614/1‑106 (MQ=255)
                                                                                                                                   caccCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACct                    <  1:1215746/150‑1 (MQ=255)
                                                                                                                                     cccAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgc                  >  1:5234573/1‑150 (MQ=255)
                                                                                                                                      ccAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgct                 <  2:3518659/150‑1 (MQ=255)
                                                                                                                                       cAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACctgctg                <  2:2762192/150‑1 (MQ=255)
                                                                                                                                          gccgacgaccGAGCCGTCGTCGTCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTcc             >  2:2381552/3‑150 (MQ=37)
                                                                                                                                             gacgacgGCTCGGTCGTCGGCCTGACCGCCGGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGt          >  1:2434486/1‑150 (MQ=255)
                                                                                                                                             gacgacgGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGt          >  1:2511034/1‑150 (MQ=255)
                                                                                                                                               cgacgGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGa        <  1:2750102/150‑1 (MQ=255)
                                                                                                                                                 acgGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAAc      <  1:1039123/150‑1 (MQ=255)
                                                                                                                                                 acgGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAAc      >  1:4332633/1‑150 (MQ=255)
                                                                                                                                                   ggCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAAcag    >  2:3325512/1‑150 (MQ=255)
                                                                                                                                                   ggCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAAcag    <  2:3247887/150‑1 (MQ=255)
                                                                                                                                                     cTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAAcagca  <  1:3639483/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTATACCATCAGACACATCAACGATAAAAACCGCGGACCACCGCAGCCCTTCAGAGGTACTCCCGATGATCGACGCAACTGTCTACAAGAACGTTCTGGGCTCCTTCCCGTCCGGCGTTACCGTCATCACCACCCAGGACGACGACGGCTCGGTCGTCGGCCTGACCGCCAGCGCCTTCTCCTCCCTGTCGATGGACCCGCCGCTGGTGCTGTTCTGCCCCAACTACACCTCCGACTCCTACCCGGTACTGATCAAGCAGAAGCGCTTTGCCATTCACCTGCTGTCCGGTGAACAGCA  >  NC_002947/4143429‑4143727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑