BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,193,343	T→C	5.9%	R82R (CGT→CGC) ‡	PP_3684 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,193,343	0	T	C	5.9%	650.8 / 18.4	202	R82R (CGT→CGC) ‡	PP_3684	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (96/94); new base C (5/7); total (101/101)
Fisher's exact test for biased strand distribution p-value = 7.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 207 total aligned reads displayed.

GTCTTCCCAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAGACA  >  NC_002947/4193194‑4193492

gTCTTCCCAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGt                                                                                                                                                       <  2:2748105/150‑1 (MQ=255)

gTCTTCCCAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGt                                                                                                                                                       >  2:4515581/1‑150 (MQ=255)

 tCTTCCCAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGCTGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTg                                                                                                                                                      >  2:3934288/1‑150 (MQ=255)

  cTTCCCAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGa                                                                                                                                                     >  2:3595639/1‑150 (MQ=255)

      ccAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGct                                                                                                                                                 >  1:1532477/1‑150 (MQ=255)

      ccAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGct                                                                                                                                                 >  1:3510599/1‑150 (MQ=255)

      ccAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGct                                                                                                                                                 >  2:203843/1‑150 (MQ=255)

      ccAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGct                                                                                                                                                 >  1:471628/1‑150 (MQ=255)

      ccAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGct                                                                                                                                                 >  1:3072481/1‑150 (MQ=255)

       cAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGctc                                                                                                                                                <  1:1773461/150‑1 (MQ=255)

       cAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGctc                                                                                                                                                >  2:3576749/1‑150 (MQ=255)

         gACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCtctc                                                                                                                                              >  1:3401457/1‑150 (MQ=255)

          aCCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCa                                                                                                                                             <  1:5478248/150‑1 (MQ=255)

          aCCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCa                                                                                                                                             <  1:469985/150‑1 (MQ=255)

            cGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCAAg                                                                                                                                           <  2:3818269/150‑1 (MQ=255)

            cGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCAAg                                                                                                                                           <  1:3225813/150‑1 (MQ=255)

            cGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAg                                                                                                                                           >  2:5565028/1‑150 (MQ=255)

             gAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGc                                                                                                                                          <  1:65624/150‑1 (MQ=255)

              aaGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCAAgga                                                                                                                                         <  2:4378480/150‑3 (MQ=255)

               aGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCAc                                                                                                                                        >  1:4148333/1‑150 (MQ=255)

                  ccAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTg                                                                                                                                     <  2:1579175/150‑1 (MQ=255)

                   cAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTgg                                                                                                                                    >  1:1682735/1‑150 (MQ=255)

                   cAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGACCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTgg                                                                                                                                    >  2:5105339/1‑150 (MQ=255)

                             gatttggCATGAGCGCCAATTCATCTAGGCGGTGTAAAGACTCTAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATTCGTGTTGTGATCCTTGAGTGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGgc                                                                                                                          <  1:3818158/145‑1 (MQ=255)

                                   gCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCg                                                                                                                    >  2:1130659/1‑150 (MQ=255)

                                   gCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCg                                                                                                                    >  1:1479466/1‑150 (MQ=255)

                                         gcgcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGc                                                                                                              <  1:615201/150‑1 (MQ=255)

                                         gcgcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGc                                                                                                              <  2:2860124/150‑1 (MQ=255)

                                          cgcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCg                                                                                                             <  1:3855345/150‑1 (MQ=255)

                                           gcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGc                                                                                                              <  1:400574/148‑1 (MQ=255)

                                                 tCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACaaaa                                                                                                      >  2:106276/1‑150 (MQ=255)

                                                  cATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAAc                                                                                                     >  2:352404/1‑150 (MQ=255)

                                                  cATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAAc                                                                                                     <  1:4518520/150‑1 (MQ=255)

                                                   aTCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAAcc                                                                                                    >  2:2954042/1‑150 (MQ=255)

                                                   aTCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAAcc                                                                                                    >  2:1936447/1‑150 (MQ=255)

                                                   aTCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAAcc                                                                                                    <  2:4994776/150‑1 (MQ=255)

                                                    ttgAGGCTGTTCACCGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCt                                                                                                   >  1:909610/3‑150 (MQ=255)

                                                    tCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCt                                                                                                   >  2:1251157/1‑150 (MQ=255)

                                                      gAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAACGCGAACAAAACCTGa                                                                                                 >  2:5191597/1‑150 (MQ=255)

                                                      gAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGa                                                                                                 <  2:5133794/150‑1 (MQ=255)

                                                      gAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGa                                                                                                 <  2:3072481/150‑1 (MQ=255)

                                                               aaCAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCtt                                                                                        <  1:4905370/150‑1 (MQ=255)

                                                                  aGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTtgtg                                                                                     <  2:1109239/150‑1 (MQ=255)

                                                                  aGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTtgtg                                                                                     <  2:748/150‑1 (MQ=255)

                                                                  aGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTtgtg                                                                                     <  1:1938309/150‑1 (MQ=255)

                                                                     cTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGtt                                                                                  >  1:5345537/1‑150 (MQ=255)

                                                                      tCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGttt                                                                                 >  1:859525/1‑150 (MQ=255)

                                                                      tCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGttt                                                                                 <  1:4037546/150‑1 (MQ=255)

                                                                      tCAAAACCGTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGttt                                                                                 >  2:1003851/1‑150 (MQ=255)

                                                                       cAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTc                                                                                <  1:4427851/150‑1 (MQ=255)

                                                                        aaaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCa                                                                               <  1:3934288/150‑1 (MQ=255)

                                                                        aaaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCa                                                                               >  1:2707893/1‑150 (MQ=255)

                                                                        aaaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCa                                                                               <  2:572535/150‑1 (MQ=255)

                                                                        aaaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCa                                                                               <  2:2362059/150‑1 (MQ=255)

                                                                         aaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAg                                                                              <  2:5323548/150‑1 (MQ=255)

                                                                         aaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAg                                                                              >  1:295248/1‑150 (MQ=255)

                                                                           aCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGAt                                                                            <  1:2042890/150‑1 (MQ=255)

                                                                              ttGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGAtctc                                                                         >  2:3460910/1‑150 (MQ=255)

                                                                                gAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCaa                                                                       <  1:961694/150‑1 (MQ=255)

                                                                                   ggTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGcc                                                                    <  1:4267557/150‑1 (MQ=255)

                                                                                   ggTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGcc                                                                    >  1:2860812/1‑150 (MQ=255)

                                                                                   ggTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGcc                                                                    >  2:179326/1‑150 (MQ=255)

                                                                                      cACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCctc                                                                 >  2:3221357/1‑150 (MQ=255)

                                                                                         tGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTaa                                                              >  2:4159864/1‑150 (MQ=255)

                                                                                          gACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACATGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTaaa                                                             <  2:1389179/150‑1 (MQ=255)

                                                                                           aCCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAAc                                                            <  1:1027805/150‑1 (MQ=255)

                                                                                           aCCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAAc                                                            >  1:2465884/1‑150 (MQ=255)

                                                                                           aCCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAAc                                                            <  1:1178889/150‑1 (MQ=255)

                                                                                             cGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTg                                                          >  1:4093997/1‑150 (MQ=255)

                                                                                                ggCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAg                                                       <  2:3777923/150‑1 (MQ=255)

                                                                                                       tGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCTGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTc                                                <  2:4095089/150‑1 (MQ=255)

                                                                                                           gatcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACt                                            <  2:2891712/150‑1 (MQ=255)

                                                                                                           gagcgaCCCTGACGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCCCTTGGTCGACCCGGCGCATCGGACAGCGAACAAAACCTGAGCGCTGCTGGGGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACt                                            >  2:5081631/4‑150 (MQ=255)

                                                                                                             tcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGc                                          <  1:3843850/150‑1 (MQ=255)

                                                                                                             tcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGc                                          <  2:3434375/150‑1 (MQ=255)

                                                                                                                  ccTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTAtt                                     >  1:5001203/1‑150 (MQ=255)

                                                                                                                    tGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTAtttt                                   >  1:2284805/1‑150 (MQ=255)

                                                                                                                     gAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTtg                                  >  2:4305518/1‑150 (MQ=255)

                                                                                                                      aaGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTtgt                                 <  2:2180196/150‑1 (MQ=255)

                                                                                                                           aTACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCt                            <  2:901613/150‑1 (MQ=255)

                                                                                                                             aCGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCttt                          >  2:5406729/1‑150 (MQ=255)

                                                                                                                              cGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTg                         <  1:4952148/150‑1 (MQ=255)

                                                                                                                                 gatgtgatCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGGTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTgcgc                      >  1:2249406/1‑150 (MQ=255)

                                                                                                                                   tgtgatCCTTGAGAGTCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTc                    >  1:3691750/1‑150 (MQ=255)

                                                                                                                                   tgtgatCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTc                    <  1:504456/150‑1 (MQ=255)

                                                                                                                                   tgtgatCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTc                    <  1:4328457/150‑1 (MQ=255)

                                                                                                                                    gtgatCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTGTGTGCCCTTTGCGCTCg                   >  2:4439873/1‑150 (MQ=255)

                                                                                                                                        tCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCaa                                              >  1:3455460/1‑119 (MQ=255)

                                                                                                                                        tCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACaa               >  1:5230817/1‑150 (MQ=255)

                                                                                                                                         gcTTGAGAGCCTCACGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAAc              >  2:3102567/2‑150 (MQ=255)

                                                                                                                                         gcTTGAGAGCCTCACGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAAc              >  2:2257118/2‑150 (MQ=255)

                                                                                                                                          cTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACt             >  2:3150262/1‑150 (MQ=255)

                                                                                                                                           ttGAGAGCCTCACGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGAACTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTc            >  1:218138/1‑150 (MQ=255)

                                                                                                                                           ttGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTc            >  2:3329673/1‑150 (MQ=255)

                                                                                                                                           ttGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTc            >  1:223445/1‑150 (MQ=255)

                                                                                                                                               gagCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGta        <  1:3150262/150‑1 (MQ=255)

                                                                                                                                               gagCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGta        <  1:1130659/150‑1 (MQ=255)

                                                                                                                                                agCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGtat       <  1:3595639/150‑1 (MQ=255)

                                                                                                                                                 gCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGtata      <  2:471628/150‑1 (MQ=255)

                                                                                                                                                   cGTGAGGATCTCAATCACATGGTAGACCCGGCGCATAGGAAAGCGAACAAAACCTGAGCGATGCTTGTGGTTTCAGATCTCAAGACCTCTAAACTGCAGAGACTTCAACTGCGTATTTTGTGCACTTTGCGCTCGACAACTCCGTATAGa    >  2:2692586/1‑150 (MQ=255)

GTCTTCCCAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAGACA  >  NC_002947/4193194‑4193492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`