BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,193,345	A→C	5.6%	E83A (GAG→GCG) ‡	PP_3684 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,193,345	0	A	C	5.6%	712.5 / 21.1	206	E83A (GAG→GCG) ‡	PP_3684	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (96/98); new base C (5/7); total (101/105)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

CTTCCCAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAGACAA  >  NC_002947/4193196‑4193493

    ccAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGct                                                                                                                                                  >  1:1532477/1‑150 (MQ=255)

    ccAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGct                                                                                                                                                  >  1:471628/1‑150 (MQ=255)

    ccAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGct                                                                                                                                                  >  1:3509957/1‑150 (MQ=255)

     cAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGctc                                                                                                                                                 <  1:1773461/150‑1 (MQ=255)

     cAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGctc                                                                                                                                                 >  2:3576851/1‑150 (MQ=255)

     cAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGctc                                                                                                                                                 <  1:5025305/150‑1 (MQ=255)

        aCCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCa                                                                                                                                              <  1:469985/150‑1 (MQ=255)

         ccGAAGACCATAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGTATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCaa                                                                                                                                             <  1:3983573/150‑1 (MQ=255)

         ccGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCaa                                                                                                                                             <  1:4144461/150‑1 (MQ=255)

          cGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAg                                                                                                                                            >  2:5565028/1‑150 (MQ=255)

          cGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAAGTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCAAg                                                                                                                                            <  2:4152681/150‑1 (MQ=255)

            aaGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCTCACGGCTCTCAAgga                                                                                                                                          <  2:4378509/150‑3 (MQ=255)

                 cagcagcagAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGTAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTgg                                                                                                                                     <  2:3117775/150‑1 (MQ=255)

                 cAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTgg                                                                                                                                     >  1:1682735/1‑150 (MQ=255)

                 cAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGACCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTgg                                                                                                                                     >  2:5105339/1‑150 (MQ=255)

                  aGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGt                                                                                                                                    >  1:647082/1‑150 (MQ=255)

                  aGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTAGGt                                                                                                                                    >  2:3930253/1‑150 (MQ=255)

                           gatttggCATGAGCGCCAATTCATCTAGGCGGTGTAAAGACTCTAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATTCGTGTTGTGATCCTTGAGTGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGgc                                                                                                                           <  1:3818158/145‑1 (MQ=255)

                                ggCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATc                                                                                                                      <  2:3669862/150‑1 (MQ=255)

                                 gCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCg                                                                                                                     >  1:1479466/1‑150 (MQ=255)

                                 gCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCg                                                                                                                     >  2:1130659/1‑150 (MQ=255)

                                       gcgcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGc                                                                                                               <  2:2860646/150‑1 (MQ=255)

                                        cgcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCg                                                                                                              <  1:2801076/150‑1 (MQ=255)

                                        cgcCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCg                                                                                                              <  1:3855345/150‑1 (MQ=255)

                                          ccAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGaa                                                                                                            <  1:3025121/150‑1 (MQ=255)

                                          caaaTTCATCGAGGCGGTGAACAGCCTCAAAACATAGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGAGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTTGTCGAACCGGCGCATCGGAAAGCGaa                                                                                                            >  1:3073642/3‑150 (MQ=255)

                                           cAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAAc                                                                                                           >  2:2706073/1‑150 (MQ=255)

                                               tCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACaaaa                                                                                                       >  2:106276/1‑150 (MQ=255)

                                                cATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAAc                                                                                                      >  2:352404/1‑150 (MQ=255)

                                                cATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAAc                                                                                                      <  1:4518520/150‑1 (MQ=255)

                                                 aTCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAAcc                                                                                                     <  2:4994776/150‑1 (MQ=255)

                                                  tCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCt                                                                                                    >  2:1251157/1‑150 (MQ=255)

                                                    gAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAACGCGAACAAAACCTGa                                                                                                  >  2:5191597/1‑150 (MQ=255)

                                                    gAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGa                                                                                                  <  2:2999448/150‑1 (MQ=255)

                                                    gAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGa                                                                                                  <  2:5133794/150‑1 (MQ=255)

                                                    gAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGa                                                                                                  <  1:3322049/150‑1 (MQ=255)

                                                     aGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAg                                                                                                 <  1:4014571/150‑1 (MQ=255)

                                                             aaCAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCtt                                                                                         <  1:3454525/150‑1 (MQ=255)

                                                                aGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTtgtg                                                                                      <  2:1109239/150‑1 (MQ=255)

                                                                aGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTtgtg                                                                                      <  2:748/150‑1 (MQ=255)

                                                                aGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTtgtg                                                                                      >  1:492285/1‑150 (MQ=255)

                                                                aGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTtgtg                                                                                      <  1:1938309/150‑1 (MQ=255)

                                                                  ccTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGt                                                                                    >  2:1886242/1‑150 (MQ=255)

                                                                   cTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGtt                                                                                   >  1:5345537/1‑150 (MQ=255)

                                                                    tCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGttt                                                                                  >  2:2227840/1‑150 (MQ=255)

                                                                    tCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGttt                                                                                  >  1:859787/1‑150 (MQ=255)

                                                                    tCAAAACCGTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGttt                                                                                  >  2:1003851/1‑150 (MQ=255)

                                                                     cAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTc                                                                                 <  1:4427851/150‑1 (MQ=255)

                                                                      aaaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCa                                                                                >  1:2707893/1‑150 (MQ=255)

                                                                      aaaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCa                                                                                <  1:3934288/150‑1 (MQ=255)

                                                                      aaaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCa                                                                                <  2:572535/150‑1 (MQ=255)

                                                                      aaaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCa                                                                                <  2:2362059/150‑1 (MQ=255)

                                                                       aaaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAg                                                                               <  2:5323548/150‑1 (MQ=255)

                                                                        aaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGa                                                                              <  2:1490913/150‑1 (MQ=255)

                                                                        aaCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGa                                                                              <  2:4327129/150‑1 (MQ=255)

                                                                         aCCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGAt                                                                             <  1:2042890/150‑1 (MQ=255)

                                                                            ttGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGAtctc                                                                          >  2:3460910/1‑150 (MQ=255)

                                                                                gggTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGc                                                                      <  1:4051600/150‑1 (MQ=255)

                                                                                    cACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCctc                                                                  >  2:3221357/1‑150 (MQ=255)

                                                                                         aCCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAAc                                                             <  1:1178889/150‑1 (MQ=255)

                                                                                         aCCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAAc                                                             <  1:1027805/150‑1 (MQ=255)

                                                                                         aCCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAAc                                                             >  1:2465884/1‑150 (MQ=255)

                                                                                           cGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTg                                                           >  2:4205655/1‑150 (MQ=255)

                                                                                              ggCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAg                                                        <  2:3777942/150‑1 (MQ=255)

                                                                                                     tGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCTGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTc                                                 <  2:4095089/150‑1 (MQ=255)

                                                                                                         gatcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACt                                             <  1:5565028/150‑1 (MQ=255)

                                                                                                         gatcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACt                                             <  1:935687/150‑1 (MQ=255)

                                                                                                         gagcgaCCCTGACGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCCCTTGGTCGACCCGGCGCATCGGACAGCGAACAAAACCTGAGCGCTGCTGGGGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACt                                             >  2:5081631/4‑150 (MQ=255)

                                                                                                           tcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGc                                           <  2:3434375/150‑1 (MQ=255)

                                                                                                           tcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGc                                           <  1:3843850/150‑1 (MQ=255)

                                                                                                           tcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGc                                           <  2:2020966/150‑1 (MQ=255)

                                                                                                           tcgaCCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGc                                           >  1:799769/1‑150 (MQ=255)

                                                                                                                ccTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTAtt                                      >  2:3074716/1‑150 (MQ=255)

                                                                                                                   gAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTtg                                   >  2:2900136/1‑150 (MQ=255)

                                                                                                                         aTACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCt                             <  2:901613/150‑1 (MQ=255)

                                                                                                                           aCGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCttt                           >  2:5406729/1‑150 (MQ=255)

                                                                                                                            cGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTg                          <  1:4952178/150‑1 (MQ=255)

                                                                                                                               gatgtgatCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGGTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTgcgc                       >  1:2249406/1‑150 (MQ=255)

                                                                                                                                 tgtgatCCTTGAGAGTCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTc                     >  1:3691750/1‑150 (MQ=255)

                                                                                                                                 tgtgatCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTc                     <  1:4328457/150‑1 (MQ=255)

                                                                                                                                 tgtgatCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTc                     <  1:504464/150‑1 (MQ=255)

                                                                                                                                  gtgatCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTGTGTGCCCTTTGCGCTCg                    >  2:4439873/1‑150 (MQ=255)

                                                                                                                                     atCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACa                 >  1:4257838/1‑150 (MQ=255)

                                                                                                                                     atCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACa                 >  1:2931573/1‑150 (MQ=255)

                                                                                                                                      tCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACaa                >  1:5230817/1‑150 (MQ=255)

                                                                                                                                       gcTTGAGAGCCTCACGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAAc               >  2:2257118/2‑150 (MQ=255)

                                                                                                                                        cTTGAGAGCCGTGAGGCTCTCAAGCACTTTGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACt              >  2:3212894/1‑150 (MQ=255)

                                                                                                                                         ttGAGAGCCTCACGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGAACTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTc             >  1:218138/1‑150 (MQ=255)

                                                                                                                                         ttGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTc             >  1:223445/1‑150 (MQ=255)

                                                                                                                                         ttGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTc             >  2:3329673/1‑150 (MQ=255)

                                                                                                                                            agagCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGt          <  2:1755244/150‑1 (MQ=255)

                                                                                                                                             gagCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGta         <  1:1130659/150‑1 (MQ=255)

                                                                                                                                             gagCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGta         <  1:3212894/150‑1 (MQ=255)

                                                                                                                                              agCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGtat        <  1:3595639/150‑1 (MQ=255)

                                                                                                                                               gCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGtata       <  2:471547/150‑1 (MQ=255)

                                                                                                                                                 cGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAGa     >  2:2084539/1‑150 (MQ=255)

                                                                                                                                                    gAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAGACaa  <  2:2493238/150‑1 (MQ=255)

                                                                                                                                                    gAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAGACaa  <  2:1603683/150‑1 (MQ=255)

                                                                                                                                                    gAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAGACaa  >  1:98742/1‑150 (MQ=255)

                                                                                                                                                    gAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAGACaa  <  1:3576749/150‑1 (MQ=255)

CTTCCCAGACCGAAGACCAGAAGCAGAGATAAGGCATGAGCGCCAATTCATCGAGGCGGTGAACAGCCTCAAAACCTTGAGGGTCACTGACCGAGGCGGGATGTCGATCGACCCTGAAGAGATACGTGATGTGATCCTTGAGAGCCGTGAGGCTCTCAAGCACTTGGTCGACCCGGCGCATCGGAAAGCGAACAAAACCTGAGCGCTGCTTGTGGTTTCAGATCTCAAGCCCTCTAAACTGCAGCGACTTCAACTGCGTATTTTGTGCCCTTTGCGCTCGACAACTCCGTATAGACAA  >  NC_002947/4193196‑4193493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`