BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,205,205	T→A	5.5%	R276* (AGA→TGA)	PP_3690 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,205,205	0	T	A	5.5%	676.2 / 19.5	199	R276* (AGA→TGA)	PP_3690	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (94/94); new base A (4/7); total (98/101)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 205 total aligned reads displayed.

CTCAAGCTGCATCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGTTGACGATTACTGAACTCCACTT  >  NC_002947/4205056‑4205354

cTCAAGCTGCATCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGctct                                                                                                                                                       <  1:4767181/150‑1 (MQ=255)

cTCAAGCTGCATCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGctct                                                                                                                                                       >  2:1378429/1‑150 (MQ=255)

 tCAAGCTGCATCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTa                                                                                                                                                      >  1:2162621/1‑150 (MQ=255)

     gCTGCATCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGa                                                                                                                                                  <  2:4000039/150‑1 (MQ=255)

     gCTGCATCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGa                                                                                                                                                  <  2:3470010/150‑1 (MQ=255)

      cTGCATCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAg                                                                                                                                                 <  2:4621389/150‑1 (MQ=255)

         cATCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCtt                                                                                                                                              <  1:3269291/150‑1 (MQ=255)

          aTCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTg                                                                                                                                             <  2:5483390/150‑1 (MQ=255)

          aTCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCAATAGAGCTTg                                                                                                                                             <  2:1337393/150‑1 (MQ=255)

           tCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGt                                                                                                                                            <  1:2212665/150‑1 (MQ=255)

             aCGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCAATAGAGCTTggca                                                                                                                                          <  2:701062/150‑4 (MQ=255)

                gCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAg                                                                                                                                       <  2:3212338/150‑1 (MQ=255)

                gCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAg                                                                                                                                       <  2:1726099/150‑1 (MQ=255)

                 cTGGCACCGCTAGCTTGGAAATTGCCGCAAAATCGTTGTCCGTTCCGAGAACTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTCCCTGCCAAGCTCAATAGAGCTTggcaggca                                                                                                                                      <  2:5290583/150‑8 (MQ=255)

                  tGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCt                                                                                                                                     <  1:1401051/150‑1 (MQ=255)

                   ggCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTg                                                                                                                                    <  2:11876/150‑1 (MQ=255)

                   ggCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTg                                                                                                                                    >  2:1785107/1‑150 (MQ=255)

                         gcttgcttGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATaa                                                                                                                              >  1:777641/1‑150 (MQ=255)

                          cttgcttgGAAATTGCGGCAAAATCGTTGTCTGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATaaa                                                                                                                             >  2:972969/1‑150 (MQ=255)

                               ttgGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACg                                                                                                                        >  2:4995408/1‑150 (MQ=255)

                               ttgGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACg                                                                                                                        >  1:5408496/1‑150 (MQ=255)

                                  gAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCt                                                                                                                     >  1:3194625/1‑150 (MQ=255)

                                  gAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCt                                                                                                                     >  2:1687655/1‑150 (MQ=255)

                                               aaTCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCaa                                                                                                        <  1:404305/150‑1 (MQ=255)

                                                    ttGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAAt                                                                                                   >  1:4263422/1‑150 (MQ=255)

                                                    gtGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAAt                                                                                                   <  1:5022921/149‑1 (MQ=255)

                                                     tGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATc                                                                                                  <  2:2416630/150‑1 (MQ=255)

                                                        ccGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGa                                                                                               >  1:3034753/1‑150 (MQ=255)

                                                        ccGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGa                                                                                               >  1:4132954/1‑150 (MQ=255)

                                                        ccGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGa                                                                                               <  2:691899/150‑1 (MQ=255)

                                                        ccGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGa                                                                                               <  2:759532/150‑1 (MQ=255)

                                                         cGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGAc                                                                                              <  1:1596774/150‑1 (MQ=255)

                                                            tCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAg                                                                                           >  2:3480512/1‑150 (MQ=255)

                                                             ccGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGt                                                                                          <  2:3882447/150‑1 (MQ=255)

                                                              cGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTa                                                                                         >  2:4084593/1‑150 (MQ=255)

                                                               gagaTCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAg                                                                                        >  2:2398685/1‑150 (MQ=255)

                                                               gagaTCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAg                                                                                        >  2:3234472/1‑150 (MQ=255)

                                                               gagaTCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAg                                                                                        >  1:5479162/1‑150 (MQ=255)

                                                                     ttCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTaaa                                                                                  <  1:5263496/150‑1 (MQ=255)

                                                                     ttCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTaaa                                                                                  >  1:2388358/1‑150 (MQ=255)

                                                                     ttCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTaaa                                                                                  >  1:2525173/1‑150 (MQ=255)

                                                                         aaGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACa                                                                              <  2:4943501/150‑1 (MQ=255)

                                                                           gTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGa                                                                            <  1:3632871/150‑1 (MQ=255)

                                                                           gTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGa                                                                            <  1:1687996/150‑1 (MQ=255)

                                                                           gTACGCCTTCAGAACGCCCTCGATACATACGATAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAgt                                                                            <  2:964711/150‑2 (MQ=255)

                                                                               gCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCg                                                                        >  1:1784334/1‑150 (MQ=255)

                                                                                  ttCAGAACGCCCTCGATACATTCGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCgatg                                                                     <  2:1025413/150‑3 (MQ=255)

                                                                                  ttCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCgaag                                                                     >  1:565874/1‑150 (MQ=255)

                                                                                  ttCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCgaag                                                                     <  1:5495091/150‑1 (MQ=255)

                                                                                    cAGAACGCCCTAGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCgaagaa                                                                   <  2:3020493/150‑1 (MQ=255)

                                                                                      gAACTCCCTCGTTACATACGCTATCGTTCAGTGCTGCCGTATCTATGCCTGCCTTCCAAGCTCTATGGAGCTTGTTCCAGCTGTATTTAAACTCTCCTCCTTCACGCTCAAAAAATCTGACGATTAGGATAAAAACAGACCCGATGAAGt                                                                 <  2:4574995/150‑1 (MQ=255)

                                                                                      gAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGt                                                                 <  2:2030203/150‑1 (MQ=255)

                                                                                       aaCGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTc                                                                <  1:4926841/150‑1 (MQ=255)

                                                                                       aaCGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTc                                                                <  1:3864668/150‑1 (MQ=255)

                                                                                       aaCGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTc                                                                >  2:3404549/1‑150 (MQ=255)

                                                                                       aaCGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTc                                                                >  2:5510687/1‑150 (MQ=255)

                                                                                        aCGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCa                                                               >  2:1360686/1‑150 (MQ=255)

                                                                                        aCGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCa                                                               <  1:4482749/150‑1 (MQ=255)

                                                                                          gCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGa                                                             <  2:5151749/150‑1 (MQ=255)

                                                                                           cccTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGaa                                                            <  2:314849/150‑1 (MQ=255)

                                                                                            ccTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAg                                                           >  2:631604/1‑150 (MQ=255)

                                                                                                gATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGcaca                                                       >  1:2924075/1‑150 (MQ=255)

                                                                                                   acatacGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAAtt                                                    <  1:1660549/150‑1 (MQ=255)

                                                                                                   acatacGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAAtt                                                    <  1:5533539/150‑1 (MQ=255)

                                                                                                     atacGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGa                                                  >  1:817949/1‑150 (MQ=255)

                                                                                                     atacGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGa                                                  >  1:4874132/1‑150 (MQ=255)

                                                                                                     atacGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGa                                                  >  2:1983233/1‑150 (MQ=255)

                                                                                                      tacGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGaa                                                 <  2:4076048/150‑1 (MQ=255)

                                                                                                      tacGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGaa                                                 <  1:209018/150‑1 (MQ=255)

                                                                                                      tacGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGaa                                                 <  1:4858625/150‑1 (MQ=255)

                                                                                                      tacGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGaa                                                 <  2:2006865/150‑1 (MQ=255)

                                                                                                          cTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGt                                             >  1:1897061/1‑150 (MQ=255)

                                                                                                           tAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTa                                            >  1:719912/1‑150 (MQ=255)

                                                                                                           tAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTa                                            <  1:2634709/150‑1 (MQ=255)

                                                                                                            aaCGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTAt                                           >  2:617299/1‑150 (MQ=255)

                                                                                                               gTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGGACAATTGAAGGGTAtctc                                        >  1:4200232/1‑150 (MQ=255)

                                                                                                               gTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTAtctc                                        <  2:4172508/150‑1 (MQ=255)

                                                                                                                   aGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACa                                    <  1:964060/150‑1 (MQ=255)

                                                                                                                     tgctgcCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAgg                                  >  1:1083198/1‑150 (MQ=255)

                                                                                                                     tgctgcCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAgg                                  >  1:2902358/1‑150 (MQ=255)

                                                                                                                       ctgcCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAATCAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCg                                >  1:3462394/1‑150 (MQ=255)

                                                                                                                         gcCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGa                              >  2:4284476/1‑150 (MQ=255)

                                                                                                                          ccGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGaa                             <  2:5323192/150‑1 (MQ=255)

                                                                                                                             aaTCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGa                          <  2:3650023/150‑1 (MQ=255)

                                                                                                                              aTCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAg                         <  2:4241979/150‑1 (MQ=255)

                                                                                                                              aTCTATGCCGGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAACACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAg                         <  2:2189309/150‑1 (MQ=255)

                                                                                                                               tCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGt                        <  2:1691735/150‑1 (MQ=255)

                                                                                                                               tCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGt                        >  2:2693675/1‑150 (MQ=255)

                                                                                                                                cTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGtt                       >  1:3232660/1‑150 (MQ=255)

                                                                                                                                     cctgcctgccAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGTTGACGa                  >  1:3123622/1‑150 (MQ=255)

                                                                                                                                      ctgcctgccAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGTTGACGAt                 >  1:787357/1‑150 (MQ=255)

                                                                                                                                      ctgcctgccAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGTTGACGAt                 >  1:4988218/1‑150 (MQ=255)

                                                                                                                                       tgcctgccAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGtt                       >  1:1912656/1‑143 (MQ=255)

                                                                                                                                       tgcctgccAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGTTGACGAtt                >  1:2199663/1‑150 (MQ=255)

                                                                                                                                        gcctgccAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGTTGACGATTa               <  1:5310830/150‑1 (MQ=255)

                                                                                                                                         tggaacAAGCTCAATAGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGTTGACGATTAc              >  2:1576674/6‑150 (MQ=255)

                                                                                                                                         tggaacAAGCTCAATAGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGTTGACGATTAc              >  2:2819762/6‑150 (MQ=255)

                                                                                                                                           gaacAAGCTCAATAGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGTTGACGATTACTg            >  1:3531638/4‑150 (MQ=255)

                                                                                                                                               aaGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGTTGACGATTACTGAACt        >  1:1759625/1‑150 (MQ=255)

                                                                                                                                                  cactAATGAGCTTGTACCAGCTGACATAAAACACCCCTCCCACACGCACAAAAAATCTGACGAGTAGGCTAAAAACACACCCGAAGAAGTCAGAAGCACAATTGAACGGTATCTCGACAGGCGGAAGGAGTTGACGATTACTGAACTCCa     <  2:4276330/148‑1 (MQ=255)

CTCAAGCTGCATCACGGCTGGCACCGCTTGCTTGGAAATTGCGGCAAAATCGTTGTCCGTTCCGAGATCTTCAAAGTACGCCTTCAGAACGCCCTCGATACATACGCTAACGTTCAGTGCTGCCGAATCTATGCCTGCCTGCCAAGCTCTATTGAGCTTGTTCCAGCTGTCATAAAACACGCCTCCGTCACGCTCAAAAAATCTGACGAGTAGGCTAAAAACAGACCCGAAGAAGTCAGAAGCACAATTGAAGGGTATCTCGACAGGCGGAAGGAGTTGACGATTACTGAACTCCACTT  >  NC_002947/4205056‑4205354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`