BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,251,178	C→G	5.9%	M30I (ATG→ATC)	alr ←	alanine racemase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,251,178	0	C	G	5.9%	809.7 / 29.7	239	M30I (ATG→ATC)	alr	alanine racemase
Reads supporting (aligned to +/- strand): ref base C (100/125); new base G (8/6); total (108/131)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 249 total aligned reads displayed.

CCATAGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTT  >  NC_002947/4251029‑4251327

  aTAGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCAt                                                                                                                                                     <  2:612701/150‑1 (MQ=255)

  aTAGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCAt                                                                                                                                                     >  2:5388991/1‑150 (MQ=255)

   tAGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATc                                                                                                                                                    <  1:1535438/150‑1 (MQ=255)

         tCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGGTGGTGCCGTTGTCCATCGACAAc                                                                                                                                              >  2:165525/1‑150 (MQ=255)

           ggCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCGATGGACAACgg                                                                                                                                            <  2:2723913/150‑1 (MQ=255)

            gCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTACCGTTGTCGATGGACAACggc                                                                                                                                           <  2:1423597/150‑2 (MQ=255)

               ttGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACggtggt                                                                                                                                        >  2:4723595/1‑150 (MQ=255)

                 gAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGc                                                                                                                                      <  2:1029582/150‑1 (MQ=255)

                  aGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGcc                                                                                                                                     <  2:4097159/150‑1 (MQ=255)

                    cACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGTAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGc                                                                                                                                   <  1:3574355/150‑1 (MQ=255)

                     aCGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCa                                                                                                                                  <  1:4505545/150‑1 (MQ=255)

                     aCGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGAGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGATGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCa                                                                                                                                  >  1:3533044/1‑150 (MQ=255)

                            acaGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAggg                                                                                                                           >  1:4195538/1‑150 (MQ=255)

                                     aCTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTGAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTc                                                                                                                  >  2:569291/1‑150 (MQ=255)

                                     aCTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTc                                                                                                                  >  2:1251307/1‑150 (MQ=255)

                                       ttGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGc                                                                                                                       >  1:3989650/1‑143 (MQ=255)

                                       ttGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGc                                                                                                                       <  2:3989741/143‑1 (MQ=255)

                                       ttGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCg                                                                                                                <  2:3682213/150‑1 (MQ=255)

                                       ttGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCg                                                                                                                >  2:5042880/1‑150 (MQ=255)

                                        tGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCgg                                                                                                               <  2:290607/150‑1 (MQ=255)

                                         gCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGt                                                                                                              <  1:3354067/150‑1 (MQ=255)

                                            ggCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGAt                                                                                                           <  1:779942/150‑1 (MQ=255)

                                             gCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATc                                                                                                          <  1:3649742/150‑1 (MQ=255)

                                              ccAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCa                                                                                                         >  1:513677/1‑150 (MQ=255)

                                                 gCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGaa                                                                                                      <  2:2874488/150‑1 (MQ=255)

                                                 gCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGaa                                                                                                      <  1:4588390/150‑1 (MQ=255)

                                                 gCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGaa                                                                                                      <  1:3023709/150‑1 (MQ=255)

                                                 gCTCGGCCTGCAGCGTGCGGATGTTGAGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGATTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGaa                                                                                                      <  1:242118/150‑1 (MQ=255)

                                                    cGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTg                                                                                                   <  2:131787/150‑1 (MQ=255)

                                                       ccTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCa                                                                                                <  2:3733574/150‑1 (MQ=255)

                                                           cAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGa                                                                                            >  2:4477769/1‑150 (MQ=255)

                                                            aGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGAt                                                                                           <  2:886121/150‑1 (MQ=255)

                                                              cGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGc                                                                                         <  1:1834521/150‑1 (MQ=255)

                                                               gTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCa                                                                                        <  1:4636477/150‑1 (MQ=255)

                                                                   ggATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCa                                                                                    <  1:4087658/150‑1 (MQ=255)

                                                                   ggATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCa                                                                                    >  2:3729755/1‑150 (MQ=255)

                                                                          gtgCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGt                                                                             >  2:1394197/1‑150 (MQ=255)

                                                                           tgctgcAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTa                                                                            <  1:4278703/150‑1 (MQ=255)

                                                                           tgctgcAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTa                                                                            <  1:5054056/150‑1 (MQ=255)

                                                                           tgctgcAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTGGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATCCAGGGGGGCCTGTCCGGTGATCAGAAGTGCCCGGGATGCAGCCAGAAGGGtc                                                                            >  1:3392991/1‑149 (MQ=255)

                                                                             ctgcAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACg                                                                          <  1:1738029/150‑1 (MQ=255)

                                                                              tgcAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACgg                                                                         >  1:2506554/1‑150 (MQ=255)

                                                                                   ggcgctggcgctGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGaaa                                                                    <  1:2588222/150‑1 (MQ=255)

                                                                                       ctggcgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGc                                                                <  1:552540/150‑1 (MQ=255)

                                                                                        tggcgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCa                                                               >  2:5071175/1‑150 (MQ=255)

                                                                                          gcgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATg                                                             <  2:4417469/150‑1 (MQ=255)

                                                                                           cgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCCGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATgg                                                            >  2:5333859/1‑150 (MQ=255)

                                                                                              tgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGa                                                         >  2:3285788/1‑150 (MQ=255)

                                                                                               gACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGAt                                                        <  2:5312869/150‑1 (MQ=255)

                                                                                               gACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGAt                                                        <  1:1792952/150‑1 (MQ=255)

                                                                                                  ttCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGAt                                                        >  2:224547/1‑147 (MQ=255)

                                                                                                  ttCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTAc                                                     >  1:3842545/1‑150 (MQ=255)

                                                                                                   tCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACt                                                    <  1:3330117/150‑1 (MQ=255)

                                                                                                    cGACCCCGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTc                                                   <  1:3237834/150‑1 (MQ=255)

                                                                                                       cccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgtg                                                >  1:2033689/1‑150 (MQ=255)

                                                                                                       cccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGAGTACTCgtg                                                >  1:4598/1‑150 (MQ=255)

                                                                                                        ccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGGATGGTGATTACTCgtgt                                               >  1:1455121/1‑150 (MQ=255)

                                                                                                        ccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgtgt                                               >  2:3051121/1‑150 (MQ=255)

                                                                                                        ccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgtgt                                               >  2:4913529/1‑150 (MQ=255)

                                                                                                              aTTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGt                                         >  2:240813/1‑150 (MQ=255)

                                                                                                              aTTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGt                                         >  1:483602/1‑150 (MQ=255)

                                                                                                                tGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCg                                       <  2:5507798/150‑1 (MQ=255)

                                                                                                                tGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCg                                       <  2:2506554/150‑1 (MQ=255)

                                                                                                                  cTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGACATGGTGATTACTCGTGTTCTTGTCGTa                                     <  1:2846570/150‑1 (MQ=255)

                                                                                                                    gTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGt                                   >  1:373383/1‑150 (MQ=255)

                                                                                                                     ttttGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTg                                  >  1:5612919/1‑150 (MQ=255)

                                                                                                                     ttttGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTg                                  >  1:3712033/1‑150 (MQ=255)

                                                                                                                         gCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTgcgcg                              >  1:2103051/1‑150 (MQ=255)

                                                                                                                          cACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTgcgcgc                             <  1:5233660/150‑1 (MQ=255)

                                                                                                                           aCGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGcc                            >  2:5240644/1‑150 (MQ=255)

                                                                                                                             ggTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCtt                          >  2:1565047/1‑150 (MQ=255)

                                                                                                                               tCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTaa                        >  2:4824311/1‑150 (MQ=255)

                                                                                                                                 aGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACa                      >  2:1872876/1‑150 (MQ=255)

                                                                                                                                  gggTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAg                     >  1:5179624/1‑150 (MQ=255)

                                                                                                                                   ggTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGa                    <  1:5240644/150‑1 (MQ=255)

                                                                                                                                   ggTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGa                    >  1:1292387/1‑150 (MQ=255)

                                                                                                                                       ttGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCa                >  2:1174606/1‑150 (MQ=255)

                                                                                                                                        tGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAg               <  1:3079791/150‑1 (MQ=255)

                                                                                                                                        tGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAg               <  1:140048/150‑1 (MQ=255)

                                                                                                                                        tGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAg               <  1:2268374/150‑1 (MQ=255)

                                                                                                                                        tGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAg               <  2:79701/150‑1 (MQ=255)

                                                                                                                                         ggTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAgg              <  2:3309507/150‑1 (MQ=255)

                                                                                                                                           tGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtg            >  2:4143940/1‑150 (MQ=255)

                                                                                                                                           caccGTTGTCGATGGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtg            >  1:2878524/3‑150 (MQ=255)

                                                                                                                                           caccGTTGTCGATGGACAACGGCGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAgg                                                                  >  2:3538612/3‑96 (MQ=255)

                                                                                                                                            accGTTGTCGATGGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtgt           >  1:1795496/2‑150 (MQ=255)

                                                                                                                                            accGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGtt                                              >  2:5544574/2‑115 (MQ=255)

                                                                                                                                            accGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGtt                                              <  1:5544574/114‑1 (MQ=255)

                                                                                                                                             ccGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtgtg          <  2:4878309/150‑1 (MQ=255)

                                                                                                                                              cGTTGTCGATGGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtgtgt         >  1:2242471/1‑150 (MQ=255)

                                                                                                                                               gTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGCGCGCGCCTTAACAGACGCAGGTGTGtt        >  1:2758240/1‑150 (MQ=255)

                                                                                                                                                ttGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGttt       <  1:165525/150‑1 (MQ=255)

                                                                                                                                                  gTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGa     <  2:1794142/150‑1 (MQ=255)

                                                                                                                                                   tCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAg    >  1:555420/1‑150 (MQ=255)

                                                                                                                                                     cATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt  >  2:4190941/1‑150 (MQ=255)

                                                                                                                                                     cATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt  >  2:3112595/1‑150 (MQ=255)

                                                                                                                                                     cATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt  >  1:1151812/1‑150 (MQ=255)

                                                                                                                                                     cATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt  >  1:542183/1‑150 (MQ=255)

                                                                                                                                                     cATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt  >  1:542134/1‑150 (MQ=255)

                                                                                                                                                     cATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGATGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt  >  1:1248452/1‑150 (MQ=255)

CCATAGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTT  >  NC_002947/4251029‑4251327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`