BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,251,181	C→G	5.7%	S29S (TCG→TCC)	alr ←	alanine racemase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,251,181	0	C	G	5.7%	833.8 / 29.6	245	S29S (TCG→TCC)	alr	alanine racemase
Reads supporting (aligned to +/- strand): ref base C (109/122); new base G (8/6); total (117/128)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 253 total aligned reads displayed.

TAGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTTCGA  >  NC_002947/4251032‑4251330

  ggCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGa                                                                                                                                                     <  1:1766892/150‑1 (MQ=255)

      tCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGGTGGTGCCGTTGTCCATCGACAAc                                                                                                                                                 >  2:165525/1‑150 (MQ=255)

        ggCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCGATGGACAACgg                                                                                                                                               <  2:2723913/150‑1 (MQ=255)

            ttGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACggtggt                                                                                                                                           >  1:1794142/1‑150 (MQ=255)

               aGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGcc                                                                                                                                        <  2:4097159/150‑1 (MQ=255)

               aGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGcc                                                                                                                                        <  2:2449865/150‑1 (MQ=255)

                  aCGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCa                                                                                                                                     <  1:4505545/150‑1 (MQ=255)

                    ggCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATa                                                                                                                                   >  1:371544/1‑150 (MQ=255)

                         acaGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAggg                                                                                                                              >  1:4195538/1‑150 (MQ=255)

                          caGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAgggg                                                                                                                             <  1:4378251/150‑1 (MQ=255)

                           aGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAggggg                                                                                                                            <  2:1258124/150‑1 (MQ=255)

                            gCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAgggggg                                                                                                                           <  1:1535285/150‑1 (MQ=255)

                            gCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAgggggg                                                                                                                           <  2:4717558/150‑1 (MQ=255)

                             cTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGc                                                                                                                          <  1:5301341/150‑1 (MQ=255)

                                    ttGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGc                                                                                                                          >  1:3989741/1‑143 (MQ=255)

                                    ttGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGc                                                                                                                          <  2:3989741/143‑1 (MQ=255)

                                    ttGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCtcatcg                                                                                                                   >  1:3912478/1‑145 (MQ=255)

                                    ttGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCg                                                                                                                   <  2:3682213/150‑1 (MQ=255)

                                    ttGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCg                                                                                                                   >  2:5042880/1‑150 (MQ=255)

                                     tGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCgg                                                                                                                  <  2:290607/150‑1 (MQ=255)

                                     tGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCgg                                                                                                                  <  2:4742002/150‑1 (MQ=255)

                                         ggCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGACCGGTGAt                                                                                                              >  1:2593954/1‑150 (MQ=255)

                                          gCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATc                                                                                                             <  1:5101749/150‑1 (MQ=255)

                                          gCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATc                                                                                                             <  1:3407674/150‑1 (MQ=255)

                                           ccAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCa                                                                                                            <  1:206173/150‑1 (MQ=255)

                                           ccAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCa                                                                                                            >  1:513677/1‑150 (MQ=255)

                                              gCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGaa                                                                                                         <  1:4588413/150‑1 (MQ=255)

                                              gCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGaa                                                                                                         <  1:1799017/150‑1 (MQ=255)

                                              gCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGaa                                                                                                         <  2:2874488/150‑1 (MQ=255)

                                              gCTCGGCCTGCAGCGTGCGGATGTTGAGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGATTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGaa                                                                                                         <  1:242118/150‑1 (MQ=255)

                                                    ccTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCa                                                                                                   <  2:3733574/150‑1 (MQ=255)

                                                       gCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAggg                                                                                                >  2:2207894/1‑150 (MQ=255)

                                                         aGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGAt                                                                                              <  2:886121/150‑1 (MQ=255)

                                                          gCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATg                                                                                             <  1:1709659/150‑1 (MQ=255)

                                                            gTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCa                                                                                           <  1:3135687/150‑1 (MQ=255)

                                                                ggATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCa                                                                                       >  2:3729755/1‑150 (MQ=255)

                                                                   tgttgtGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGaa                                                                                    >  1:4739518/1‑150 (MQ=255)

                                                                   tgttgtGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGaa                                                                                    <  1:3767534/150‑1 (MQ=255)

                                                                   tgttgtGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGaa                                                                                    <  1:2905165/150‑1 (MQ=255)

                                                                     ttgtGGTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCTCGGTCAGGGTGTTGGTGCCGTTGTCCATCGACCTCGGTGGTGCCGCATACAGGGGGGGCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAgg                                                                                  <  2:4296413/150‑1 (MQ=255)

                                                                       gtgCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGt                                                                                >  1:3347725/1‑150 (MQ=255)

                                                                        tgctgcAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTa                                                                               >  2:1405099/1‑150 (MQ=255)

                                                                                ggcgctggcgctGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGaaa                                                                       <  1:1961818/150‑1 (MQ=255)

                                                                                     tggcgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCa                                                                  >  2:5071175/1‑150 (MQ=255)

                                                                                        cgctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATgg                                                               >  2:5333841/1‑150 (MQ=255)

                                                                                          ctgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTg                                                             >  1:338935/1‑150 (MQ=255)

                                                                                           tgACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGa                                                            >  2:3285788/1‑150 (MQ=255)

                                                                                            gACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGAt                                                           <  2:5312639/150‑1 (MQ=255)

                                                                                               ttCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGaa                                                                                                         <  1:1586488/101‑1 (MQ=255)

                                                                                                 cGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTc                                                      <  2:1497554/150‑1 (MQ=255)

                                                                                                   aCCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTGGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgt                                                    >  1:3057682/1‑150 (MQ=255)

                                                                                                    cccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGAGTACTCgtg                                                   >  1:4598/1‑150 (MQ=255)

                                                                                                     ccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTc                                                      <  1:3590363/146‑1 (MQ=255)

                                                                                                     ccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgtgt                                                  >  2:3051121/1‑150 (MQ=255)

                                                                                                     ccAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCgtgt                                                  >  2:4913529/1‑150 (MQ=255)

                                                                                                         gCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCtt                                              <  2:5620257/150‑1 (MQ=255)

                                                                                                         gCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCtt                                              <  2:758573/150‑1 (MQ=255)

                                                                                                          cATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTg                                             <  1:5198781/150‑1 (MQ=255)

                                                                                                           aTTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGt                                            >  2:240813/1‑150 (MQ=255)

                                                                                                           aTTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGt                                            >  1:483602/1‑150 (MQ=255)

                                                                                                           aTTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGt                                            >  1:5515453/1‑150 (MQ=255)

                                                                                                           aTTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGATGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGCCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGt                                            <  2:1247472/150‑1 (MQ=255)

                                                                                                             tGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCg                                          <  2:1345316/150‑1 (MQ=255)

                                                                                                               cTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGACATGGTGATTACTCGTGTTCTTGTCGTa                                        <  1:2846704/150‑1 (MQ=255)

                                                                                                               cTGTTTTGCACGGTCAGGGTGTTGATGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTa                                        <  2:4455268/150‑1 (MQ=255)

                                                                                                                  ttttGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTg                                     >  1:5612919/1‑150 (MQ=255)

                                                                                                                  ttttGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTg                                     >  1:3712030/1‑150 (MQ=255)

                                                                                                                       cACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTgcgcgc                                <  1:5233660/150‑1 (MQ=255)

                                                                                                                         cGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCt                              <  1:2572548/150‑1 (MQ=255)

                                                                                                                          ggTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCtt                             >  2:1565047/1‑150 (MQ=255)

                                                                                                                            tCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTaa                           >  2:4824311/1‑150 (MQ=255)

                                                                                                                              aGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACa                         >  2:1872876/1‑150 (MQ=255)

                                                                                                                                     tGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAg                  <  1:415126/150‑1 (MQ=255)

                                                                                                                                     tGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAg                  <  1:140048/150‑1 (MQ=255)

                                                                                                                                      ggTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAgg                 <  2:3309507/150‑1 (MQ=255)

                                                                                                                                       gTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgt                <  1:2369893/150‑1 (MQ=255)

                                                                                                                                        tGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtg               >  2:4143940/1‑150 (MQ=255)

                                                                                                                                        caccGTTGTCGATGGACAACGGCGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCGGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtg               >  2:106450/3‑150 (MQ=255)

                                                                                                                                        caccGTTGTCGATGGACAACGGCGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAgg                                                                     <  1:3538612/94‑1 (MQ=255)

                                                                                                                                        caccGTTGTCGATGGACAACGGCGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAgg                                                                     >  2:3538612/3‑96 (MQ=255)

                                                                                                                                        caccGTTGTCGATGGACAACGGCGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtg               >  2:5382012/3‑150 (MQ=255)

                                                                                                                                         accGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGtt                                                 <  1:5544574/114‑1 (MQ=255)

                                                                                                                                          ccGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtgtg             <  2:4878309/150‑1 (MQ=255)

                                                                                                                                           cGTTGTCGATGGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtgtgt            >  1:2242471/1‑150 (MQ=255)

                                                                                                                                           cGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGgtgtgt            >  1:5579039/1‑150 (MQ=255)

                                                                                                                                            gTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGtt           <  1:3292093/150‑1 (MQ=255)

                                                                                                                                            gTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCAGGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGtt           >  1:2772298/1‑150 (MQ=255)

                                                                                                                                             ttGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGttt          <  2:605184/150‑1 (MQ=255)

                                                                                                                                             ttGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGttt          <  2:553368/150‑1 (MQ=255)

                                                                                                                                               gTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGa        <  2:1794081/150‑1 (MQ=255)

                                                                                                                                               gTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGa        <  1:989122/150‑1 (MQ=255)

                                                                                                                                                  cATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt     >  2:3112595/1‑150 (MQ=255)

                                                                                                                                                  cATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt     >  1:1151812/1‑150 (MQ=255)

                                                                                                                                                  cATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt     >  1:542183/1‑150 (MQ=255)

                                                                                                                                                  cATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt     >  2:4190941/1‑150 (MQ=255)

                                                                                                                                                  cATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGATGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGtt     >  1:1248452/1‑150 (MQ=255)

                                                                                                                                                   aTCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTTc    >  2:5126800/1‑150 (MQ=255)

                                                                                                                                                     cGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTTCGa  <  1:2191345/150‑1 (MQ=255)

                                                                                                                                                     cGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTTCGa  <  1:1347785/150‑1 (MQ=255)

                                                                                                                                                     cGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTTCGa  >  1:5419020/1‑150 (MQ=255)

TAGGCATCGGCCTTGAGCACGGCGCACAGCTTGGACTTGCCGGCCAGCTCGGCCTGCAGCGTGCGGATGTTGTGCTGCAGGGCGCTGGCGCTGACTTCGACCCAGGCATTGCTGTTTTGCACGGTCAGGGTGTTGGTGCCGTTGTCCATCGACAACGGTGGTGCCGCATACAGGGGGGCCTGTCCGGTGATCAGAAGTGCCAGGGATGCAGCCAGAAGGGTACGGCGAAAGGGCATGGTGATTACTCGTGTTCTTGTCGTAGTGCGCGCCTTAACAGACGCAGGTGTGTTTGAGTTCGA  >  NC_002947/4251032‑4251330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`