BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,581,040	T→C	5.3%	H148H (CAT→CAC)	ybhP‑II →	phosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,581,040	0	T	C	5.3%	726.8 / 18.2	224	H148H (CAT→CAC)	ybhP‑II	phosphohydrolase
Reads supporting (aligned to +/- strand): ref base T (113/98); new base C (8/4); total (122/102)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 233 total aligned reads displayed.

GCGGTCTACCCTGACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCT  >  NC_002947/4580891‑4581189

            gACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTg                                                                                                                                           <  2:2387526/150‑1 (MQ=255)

            gACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTg                                                                                                                                           <  2:3541258/150‑1 (MQ=255)

             aCGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGc                                                                                                                                          <  1:743051/150‑1 (MQ=255)

             aCGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGc                                                                                                                                          <  2:5015837/150‑1 (MQ=255)

              cGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCa                                                                                                                                         <  1:244475/150‑1 (MQ=255)

               ggCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCACGCAGACCGCATGCAc                                                                                                                                        <  1:5543228/150‑1 (MQ=255)

               ggCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAACGACAGGTGCACGCAGACCGCATGCAc                                                                                                                                        <  1:5381485/150‑1 (MQ=255)

                  caccacGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTg                                                                                                                                     <  1:3351303/150‑1 (MQ=255)

                   accacGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGt                                                                                                                                    >  1:753751/1‑150 (MQ=255)

                    ccacGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTc                                                                                                                                   >  1:678979/1‑150 (MQ=255)

                    ccacGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTc                                                                                                                                   >  1:2944394/1‑150 (MQ=255)

                    ccacGGCAATGCGTTGCTGTCCAAAGACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTc                                                                                                                                   <  2:3401502/150‑1 (MQ=255)

                     aacGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCg                                                                                                                                  >  1:1609734/2‑150 (MQ=255)

                      acGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGt                                                                                                                                 >  2:3980332/1‑150 (MQ=255)

                           aaTGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTg                                                                                                                            >  2:3737720/1‑150 (MQ=255)

                           aaTGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTg                                                                                                                            <  1:4606591/150‑1 (MQ=255)

                              gCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGCGCACCTGTCGTTGCTGGaa                                                                                                                         <  2:402809/150‑1 (MQ=255)

                               cGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGaaa                                                                                                                        <  2:5251151/150‑1 (MQ=255)

                               cGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGaaa                                                                                                                        <  1:1982748/150‑1 (MQ=255)

                                gTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAg                                                                                                                       >  2:3710791/1‑150 (MQ=255)

                                 ttGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGc                                                                                                                      >  2:3084908/1‑150 (MQ=255)

                                      gTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCg                                                                                                                 >  2:2076824/1‑150 (MQ=255)

                                          aaaTACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCaa                                                                                                             >  2:4626056/1‑150 (MQ=255)

                                          aaaTACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCaa                                                                                                             >  2:1523745/1‑150 (MQ=255)

                                            aTACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCaaaa                                                                                                           >  2:429338/1‑150 (MQ=255)

                                               cccGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAagca                                                                                                        >  1:2524953/1‑150 (MQ=255)

                                                ccGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAagcag                                                                                                       >  2:2619899/1‑150 (MQ=255)

                                                ccGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAagcag                                                                                                       <  1:1924669/150‑1 (MQ=255)

                                                ccGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAagcag                                                                                                       >  2:541813/1‑150 (MQ=255)

                                                ccGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAagcag                                                                                                       <  2:3368513/150‑1 (MQ=255)

                                                 cGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAagcagc                                                                                                      >  1:2360959/1‑150 (MQ=255)

                                                  gctcatcGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGACAGCCACCGGCAAAAGCAGCt                                                                                                     >  1:5061780/3‑150 (MQ=255)

                                                  gATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCt                                                                                                     >  2:5098504/1‑150 (MQ=255)

                                                   atcatcGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTg                                                                                                    <  2:476516/150‑1 (MQ=255)

                                                    tcatcGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGc                                                                                                   <  1:5347039/150‑1 (MQ=255)

                                                    tcatcGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGc                                                                                                   <  1:2624782/150‑1 (MQ=255)

                                                       tcGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGt                                                                                                >  2:613440/1‑150 (MQ=255)

                                                           gCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTtgct                                                                                            <  1:4777153/150‑1 (MQ=255)

                                                            cACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTtgctg                                                                                           <  2:3232143/150‑1 (MQ=255)

                                                            cACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTtgctg                                                                                           <  1:3328359/150‑1 (MQ=255)

                                                                gCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCCCCGGCAAAAGCAGCTGCAGTTGCTGCGTa                                                                                       >  2:5279110/1‑150 (MQ=255)

                                                                  aaCCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTaaa                                                                                     <  1:1297205/150‑1 (MQ=255)

                                                                      tGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGGAAActgc                                                                                 >  1:3785857/1‑150 (MQ=255)

                                                                       ggACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTCAActgct                                                                                >  2:3685244/1‑150 (MQ=255)

                                                                       ggACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAActgct                                                                                >  2:1023462/1‑150 (MQ=255)

                                                                         aCGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTgg                                                                              <  1:405022/150‑1 (MQ=255)

                                                                             gtCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGc                                                                          >  1:4319278/1‑150 (MQ=255)

                                                                              tCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGcc                                                                         >  2:2908465/1‑150 (MQ=255)

                                                                              tCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGcc                                                                         <  1:3709002/150‑1 (MQ=255)

                                                                                gATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCt                                                                       >  2:1127093/1‑150 (MQ=255)

                                                                                gATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCt                                                                       >  2:828747/1‑150 (MQ=255)

                                                                                gATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCt                                                                       >  1:3090249/1‑150 (MQ=255)

                                                                                   cACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGcc                                                                    <  1:2538617/150‑1 (MQ=255)

                                                                                    aCCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCg                                                                   <  2:5352293/150‑1 (MQ=255)

                                                                                      cGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGc                                                                 <  1:1123554/150‑1 (MQ=255)

                                                                                      cGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGc                                                                 >  1:3946637/1‑150 (MQ=255)

                                                                                        gCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCa                                                               >  1:1632533/1‑150 (MQ=255)

                                                                                           ccGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACg                                                            >  2:1434394/1‑150 (MQ=255)

                                                                                             gAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAACAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGcc                                                          >  2:1753231/1‑150 (MQ=255)

                                                                                              aaCGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCTAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGccc                                                         <  1:4101565/150‑1 (MQ=255)

                                                                                              aaCGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGccc                                                         <  1:4443252/150‑1 (MQ=255)

                                                                                                 gccgcGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCgg                                                      >  1:5509853/1‑150 (MQ=255)

                                                                                                    gctgCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGa                                                   >  1:1032034/4‑150 (MQ=255)

                                                                                                    gcgGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCTGGTGa                                                   <  1:1986699/150‑1 (MQ=255)

                                                                                                    gcgGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGa                                                   <  2:2082476/150‑1 (MQ=255)

                                                                                                    gcgGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGa                                                   <  1:4666432/150‑1 (MQ=255)

                                                                                                    gcgGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGa                                                   <  2:4913726/150‑1 (MQ=255)

                                                                                                     cgGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGat                                                  >  2:1666907/1‑150 (MQ=255)

                                                                                                          tGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACTTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCg                                             >  1:2218961/1‑150 (MQ=255)

                                                                                                            ttGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGcc                                           >  1:3776958/1‑150 (MQ=255)

                                                                                                              gCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCgg                                         >  1:1135361/1‑150 (MQ=255)

                                                                                                               cACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGc                                        <  2:3469954/150‑1 (MQ=255)

                                                                                                                    tgtgCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACtt                                   <  1:1564462/150‑1 (MQ=255)

                                                                                                                        cTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAAc                               >  1:136303/1‑150 (MQ=255)

                                                                                                                        cTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAAc                               <  1:4419878/150‑1 (MQ=255)

                                                                                                                         tGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACg                              >  2:4808054/1‑150 (MQ=255)

                                                                                                                          ggAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGa                             >  1:3469318/1‑150 (MQ=255)

                                                                                                                          ggAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGa                             >  1:3225205/1‑150 (MQ=255)

                                                                                                                            aGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATAATCGCCGGCGACTTCAACGACt                           <  1:595752/150‑1 (MQ=255)

                                                                                                                             ggTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTg                          <  2:302517/150‑1 (MQ=255)

                                                                                                                              gTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTgg                         >  2:3550909/1‑150 (MQ=255)

                                                                                                                              gTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTgg                         >  1:2099848/1‑150 (MQ=255)

                                                                                                                               tGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGa                        >  1:4990408/1‑150 (MQ=255)

                                                                                                                                 ccGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGaaa                      >  1:3671754/1‑150 (MQ=255)

                                                                                                                                  cGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGaaaa                     >  2:1495924/1‑150 (MQ=255)

                                                                                                                                   ggggCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAg                    >  2:2837188/1‑150 (MQ=255)

                                                                                                                                      gCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCa                 >  2:3006779/1‑150 (MQ=255)

                                                                                                                                      gCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCa                 <  2:678979/150‑1 (MQ=255)

                                                                                                                                      gCAACGACAGGTGCACGCAGACCGCATGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCa                 >  2:4462252/1‑150 (MQ=255)

                                                                                                                                      gCAACGACAGGTGCACGCAGACCGCATGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGCTGCTGCGTAAACTTCTGGACGCCCTGCCGGCCATCGCACCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCa                 >  2:1763032/1‑150 (MQ=255)

                                                                                                                                       cAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCAt                <  1:3448341/150‑1 (MQ=255)

                                                                                                                                       cAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCAt                <  2:1114147/150‑1 (MQ=255)

                                                                                                                                              aGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAAcc         <  2:190783/150‑1 (MQ=255)

                                                                                                                                               ggTGCACGCAGACCGCATGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCg        >  1:1293321/1‑150 (MQ=255)

                                                                                                                                               ggTGCACGCAGACCGCATGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCg        >  2:2321772/1‑150 (MQ=255)

                                                                                                                                                gTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGc       <  2:2534905/150‑1 (MQ=255)

                                                                                                                                                gTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGc       >  1:505415/1‑150 (MQ=255)

                                                                                                                                                  gCATGCGGTCTTCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCAc     >  2:539936/1‑150 (MQ=255)

                                                                                                                                                     tGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCTGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCt  <  1:127297/150‑1 (MQ=255)

                                                                                                                                                     tGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCt  >  1:5207117/1‑150 (MQ=255)

GCGGTCTACCCTGACGGCCACCACGGCAATGCGTTGCTGTCCAAATACCCGATCATCGAGCACCGCAACCTGGACGTGTCGATCACCGGCCCCGAACGCCGCGGCCTGTTGCACTGTGTGCTGGAGGTGCCGGGGCAAGGCCAGGTGCATGCGGTCTGCGTGCACCTGTCGTTGCTGGAAAGCCACCGGCAAAAGCAGCTGCAGTTGCTGCGTAAACTGCTGGACGCCCTGCCGGCCAACGCCCCGGTGATCATCGCCGGCGACTTCAACGACTGGAAAAGCCATGGCAACCGCACCCT  >  NC_002947/4580891‑4581189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`