BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,602,746	C→A	5.2%	Q686H (CAG→CAT) ‡	PP_4071 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,602,746	0	C	A	5.2%	789.9 / 23.1	231	Q686H (CAG→CAT) ‡	PP_4071	membrane protein
Reads supporting (aligned to +/- strand): ref base C (99/120); new base A (8/4); total (107/124)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 246 total aligned reads displayed.

TCCTTGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAG  >  NC_002947/4602597‑4602892

tCCTTGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCctgc                                                                                                                                                    <  2:2767696/150‑1 (MQ=255)

   ttGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCtgctgc                                                                                                                                                 >  2:586860/1‑150 (MQ=255)

   ttGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCtgctgc                                                                                                                                                 >  1:1478635/1‑150 (MQ=255)

      aaGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTc                                                                                                                                              <  1:64148/150‑1 (MQ=255)

      aaGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTc                                                                                                                                              >  1:5383208/1‑150 (MQ=255)

      aaGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTc                                                                                                                                              >  1:2404184/1‑150 (MQ=255)

       aGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCa                                                                                                                                             <  1:3992356/150‑1 (MQ=255)

       aGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCa                                                                                                                                             >  2:3607890/1‑150 (MQ=255)

        gCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAg                                                                                                                                            <  2:3048920/150‑1 (MQ=255)

         ccTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAgg                                                                                                                                           <  2:168763/150‑1 (MQ=255)

          cTTTGGCAGTGAGCATTGGCGCCAGCAAATCACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGACCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGc                                                                                                                                          <  2:76852/150‑1 (MQ=255)

          cTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGc                                                                                                                                          >  1:3503459/1‑150 (MQ=255)

           tttGGCAGTGAGCATTGGCGCCAGAAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGATCATGGGCCTGCTGCTTCAGGcc                                                                                                                                         >  2:1365411/1‑150 (MQ=255)

               gCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCa                                                                                                                                     <  2:4892197/150‑1 (MQ=255)

               gCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCa                                                                                                                                     <  1:4477367/150‑1 (MQ=255)

                cAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGAAGCAGCAGGcccagga                                                                                                                                    <  1:90720/150‑5 (MQ=255)

                  gTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCGGGCCCGCATac                                                                                                                                  <  1:3099870/150‑1 (MQ=255)

                    gAGCATTGGCTCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATacac                                                                                                                                <  1:3359198/150‑1 (MQ=255)

                    gAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATacac                                                                                                                                <  1:3836825/150‑1 (MQ=255)

                      gCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACAcgc                                                                                                                              >  2:4188802/1‑150 (MQ=255)

                           ggCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                         <  2:4774454/150‑1 (MQ=255)

                           ggCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                         <  1:3615093/150‑1 (MQ=255)

                           ggCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgt                                                                                                                         <  1:2140348/150‑1 (MQ=255)

                            gcgcCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTtgtg                                                                                                                        >  2:2519254/1‑150 (MQ=255)

                                    aaaaCACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGc                                                                                                                >  1:4843342/1‑150 (MQ=255)

                                    aaaaCACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGc                                                                                                                >  1:25035/1‑150 (MQ=255)

                                    aaaaCACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGc                                                                                                                >  1:3165455/1‑150 (MQ=255)

                                       acacCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCAcc                                                                                                             <  1:2894497/150‑1 (MQ=255)

                                        cacCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCt                                                                                                            <  1:2550560/150‑1 (MQ=255)

                                         acCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCtt                                                                                                           >  2:5502274/1‑150 (MQ=255)

                                         acCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCtt                                                                                                           >  1:3409335/1‑150 (MQ=255)

                                         acCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTAGTGGCAACGGCACCtt                                                                                                           >  1:5555411/1‑150 (MQ=255)

                                                tCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAAt                                                                                                    <  2:3475179/150‑1 (MQ=255)

                                                tCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAAt                                                                                                    <  1:5502274/150‑1 (MQ=255)

                                                tCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAAt                                                                                                    >  1:101228/1‑150 (MQ=255)

                                                  gCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGt                                                                                                  >  1:2963870/1‑150 (MQ=255)

                                                   cccTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTc                                                                                                 >  1:5258833/1‑150 (MQ=255)

                                                     cTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTctg                                                                                               <  1:1451706/150‑1 (MQ=255)

                                                     cTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTctg                                                                                               >  1:1247316/1‑150 (MQ=255)

                                                      tGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTctgc                                                                                              <  1:3665369/150‑1 (MQ=255)

                                                         cTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCtgctgc                                                                                           <  2:4574386/150‑1 (MQ=255)

                                                          tCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCa                                                                                          >  1:4440666/1‑150 (MQ=255)

                                                             aGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGt                                                                                       <  1:5630838/150‑1 (MQ=255)

                                                                 ggAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCt                                                                                   <  2:5246778/150‑1 (MQ=255)

                                                                 ggAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCt                                                                                   >  1:2832230/1‑150 (MQ=255)

                                                                  gAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTg                                                                                  >  2:3215267/1‑150 (MQ=255)

                                                                       cAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACg                                                                             <  1:2046894/150‑1 (MQ=255)

                                                                       cAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACg                                                                             <  2:3165455/150‑1 (MQ=255)

                                                                           tGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTCTGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCCACGGCACCGTACGCAATGTCTGCTGCACGTGGCTGACACGCTgg                                                                         >  2:4855339/1‑150 (MQ=255)

                                                                           tGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTgg                                                                         <  1:2268960/150‑1 (MQ=255)

                                                                              tAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACTCAATGTCTGCTGCACGTGGCTGACACGCTGGCGa                                                                      <  1:709802/150‑1 (MQ=255)

                                                                              tAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGa                                                                      <  1:709674/150‑1 (MQ=255)

                                                                                   gACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTg                                                                 <  2:2832366/150‑1 (MQ=255)

                                                                                    aCATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGc                                                                <  2:3713925/150‑1 (MQ=255)

                                                                                    aCATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGc                                                                >  1:4283302/1‑150 (MQ=255)

                                                                                     cATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCg                                                               <  2:3407359/150‑1 (MQ=255)

                                                                                     cATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCg                                                               <  1:2156129/150‑1 (MQ=255)

                                                                                      aTCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACGGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCgg                                                              <  2:765203/150‑1 (MQ=255)

                                                                                       tCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGc                                                             >  2:4744098/1‑150 (MQ=255)

                                                                                        cGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGAAACTGCGGCa                                                            <  2:5116416/150‑1 (MQ=255)

                                                                                         gAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAg                                                           <  2:101228/150‑1 (MQ=255)

                                                                                         gAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAg                                                           >  1:1135258/1‑150 (MQ=255)

                                                                                         gAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAg                                                           >  2:672212/1‑150 (MQ=255)

                                                                                          aaCGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAgg                                                          >  1:2452857/1‑150 (MQ=255)

                                                                                           aCGCAGGCCCCCCCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGt                                                         <  2:4114234/150‑1 (MQ=255)

                                                                                                  ccccACCTGATGACGCAGGTCCAGTCCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATc                                                  <  2:260948/150‑1 (MQ=255)

                                                                                                   cccACCTGATGACACAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATcc                                                 >  2:634949/1‑150 (MQ=255)

                                                                                                        cTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTa                                            <  1:1627250/150‑1 (MQ=255)

                                                                                                          gatgaCGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAgg                                          <  2:243881/150‑1 (MQ=255)

                                                                                                          gatgaCGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAgg                                          >  2:5608847/1‑150 (MQ=255)

                                                                                                          gatgaCGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAgg                                          >  1:1300798/1‑150 (MQ=255)

                                                                                                               cGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCg                                                       >  1:1792350/1‑132 (MQ=255)

                                                                                                               cGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAgc                                     <  2:2040254/150‑1 (MQ=255)

                                                                                                               cGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAgc                                     <  1:5092659/150‑1 (MQ=255)

                                                                                                                gCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAgcg                                    <  2:5383208/150‑1 (MQ=255)

                                                                                                                gCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAgcg                                    >  1:567592/1‑150 (MQ=255)

                                                                                                                gCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAgcg                                    >  2:3291612/1‑150 (MQ=255)

                                                                                                                gCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTCGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAgcg                                    <  1:225963/150‑1 (MQ=255)

                                                                                                                     tCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATAc                               >  2:4335459/1‑150 (MQ=255)

                                                                                                                       cAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACtt                             >  1:4610708/1‑150 (MQ=255)

                                                                                                                        aGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTc                            <  1:4080896/150‑1 (MQ=255)

                                                                                                                         gACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCa                           <  1:4188802/150‑1 (MQ=255)

                                                                                                                            cGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGt                        <  1:634874/150‑1 (MQ=255)

                                                                                                                               tATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGcc                     <  2:514324/150‑1 (MQ=255)

                                                                                                                               tATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGcc                     >  1:1870370/1‑150 (MQ=255)

                                                                                                                                   cAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCAt                 <  2:2404184/150‑1 (MQ=255)

                                                                                                                                     aCTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGa               <  2:1478635/150‑1 (MQ=255)

                                                                                                                                     aCTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGa               <  1:3248546/150‑1 (MQ=255)

                                                                                                                                         cTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtc           <  2:3865652/150‑1 (MQ=255)

                                                                                                                                          tCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtct          >  2:473857/1‑150 (MQ=255)

                                                                                                                                           tgcgggCCTGAAGCAGCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtctc         >  2:1912574/4‑150 (MQ=255)

                                                                                                                                           tgcgggCCTGAAGCAGCAGGCCCACATACACGCGTTGTGGCAACTGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtctc         >  2:3318154/4‑150 (MQ=255)

                                                                                                                                           ccTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtctc         <  2:208993/150‑1 (MQ=255)

                                                                                                                                           ccTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtctc         >  2:2203839/1‑150 (MQ=255)

                                                                                                                                           ccTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtctc         <  1:3607890/150‑1 (MQ=255)

                                                                                                                                           ccTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGtctc         <  1:262705/150‑1 (MQ=255)

                                                                                                                                              gggCCTGAAGCAGCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGtt      >  1:4366145/1‑150 (MQ=255)

                                                                                                                                              gggCCTGAAGCAGCAGGCCCACATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGtt      >  1:1074506/1‑150 (MQ=255)

                                                                                                                                                 ccTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCa   <  1:305017/150‑1 (MQ=255)

                                                                                                                                                  ctgctgCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAg  <  1:2758154/150‑1 (MQ=255)

TCCTTGAAGCCTTTGGCAGTGAGCATTGGCGCCAGCAAAACACCATTGTCGCCCTGGCTCGAGCCGGAAGACAGCTGGTAAACGACATCGAACGCAGGCCCCACCTGATGACGCAGGTCCAGACCGGTATGCAACTGCTCCTGGGCCTGCTGCTTCAGGCCCGCATACACGCGTTGTGGCAACGGCACCTTACGCAATGTCTGCTGCACGTGGCTGACACGCTGGCGATACTGCGGCAGGTCGGTATCCGCGTAGGTCAGCGCATACTTCAGGTGCCGCATGAGGTCTCGTTGCAG  >  NC_002947/4602597‑4602892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`