Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 385,478 A→C 5.7% intergenic (+15/+20) PP_0320 → / ← ltaE methyl‑accepting chemotaxis transducer/low specificity l‑threonine aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947385,4780AC 5.7% 624.1 / 14.1 196intergenic (+15/+20)PP_0320/ltaEmethyl‑accepting chemotaxis transducer/low specificity l‑threonine aldolase
Reads supporting (aligned to +/- strand):  ref base A (99/84);  new base C (4/9);  total (103/93)
Fisher's exact test for biased strand distribution p-value = 1.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

AACCGGACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGCCCCG  >  NC_002947/385329‑385626
                                                                                                                                                     |                                                                                                                                                    
aaCCGGACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCa                                                                                                                                                      >  1:1359322/1‑150 (MQ=255)
aaCCGGACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCa                                                                                                                                                      >  2:487948/1‑150 (MQ=255)
    ggACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCAcggc                                                                                                                                                  <  2:2363842/150‑1 (MQ=255)
    ggACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCAcggc                                                                                                                                                  <  1:4678618/150‑1 (MQ=255)
     gACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCAcggcg                                                                                                                                                 >  1:3434479/1‑150 (MQ=255)
     gACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCAcggcg                                                                                                                                                 <  1:4591978/150‑1 (MQ=255)
          tGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGcccc                                                                                                                                            >  1:3230156/1‑150 (MQ=255)
          tGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGcccc                                                                                                                                            <  2:4180029/150‑1 (MQ=255)
          tGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGcccc                                                                                                                                            <  2:5029211/150‑1 (MQ=255)
            cAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCgg                                                                                                                                          <  1:4796131/150‑1 (MQ=255)
             aGGGGATGTCCGATTGGGCGCGTGAGCCGGCGGCGGAGGGGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCCGCTGGGTGGTTCCCGGCTCTAGCTCGCAGGGGCCGCACGGCGGCCCCGGc                                                                                                                                         <  1:175087/150‑1 (MQ=255)
              ggggATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGct                                                                                                                                        >  1:1860614/1‑150 (MQ=255)
                 gATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCCGGGGCCGCCGTGCGGCCCCTGcgatc                                                                                                                                     <  1:2770851/150‑5 (MQ=255)
                     tCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTc                                                                                                                                 >  2:51895/1‑150 (MQ=255)
                     tCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTc                                                                                                                                 >  2:2809403/1‑150 (MQ=255)
                     tCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTc                                                                                                                                 >  2:2863366/1‑150 (MQ=255)
                     tCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTc                                                                                                                                 >  1:1635369/1‑150 (MQ=255)
                      ccGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCa                                                                                                                                <  2:3107478/150‑1 (MQ=255)
                      ccGATTGGGCGCGTGAGACGGCGGCGGAGGTGCACGGGTGCCGCGAGGAGGGCCAGGCGTTGCGTGGGTGGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCa                                                                                                                                <  2:1376879/150‑1 (MQ=255)
                          tttGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGcca                                                                                                                            <  2:5387055/150‑1 (MQ=255)
                             ggCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGccacca                                                                                                                         >  1:3012869/1‑150 (MQ=255)
                                gcgTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCCGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCCCCAATg                                                                                                                      >  1:2403266/1‑150 (MQ=255)
                                gcgTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATg                                                                                                                      >  1:687518/1‑150 (MQ=255)
                                 cgTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGa                                                                                                                     <  1:927806/150‑1 (MQ=255)
                                     agaCGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGt                                                                                                                 >  1:2610236/1‑150 (MQ=255)
                                         ggcggcggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCgg                                                                                                             >  1:497289/1‑150 (MQ=255)
                                         ggcggcggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCgg                                                                                                             >  1:2289438/1‑150 (MQ=255)
                                             gcggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGatat                                                                                                         >  2:2521663/1‑150 (MQ=255)
                                               ggAGGTGCAAGGGTGCCGCGAGGCGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGAGGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATcc                                                                                                       <  2:3997103/150‑1 (MQ=255)
                                               ggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATcc                                                                                                       >  1:248508/1‑150 (MQ=255)
                                               ggAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATcc                                                                                                       <  2:1771458/150‑1 (MQ=255)
                                                ctggTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATccg                                                                                                      >  2:824692/3‑150 (MQ=255)
                                                 aGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATccgc                                                                                                     <  2:4480226/150‑1 (MQ=255)
                                                  ggTGCAAGGGTGCCGCGTGGAGTGCCTGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCTGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATccgcc                                                                                                    <  2:2614911/150‑1 (MQ=255)
                                                    tGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCcgccgc                                                                                                  <  2:5584007/150‑1 (MQ=255)
                                                      cAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCa                                                                                                >  1:4708991/1‑150 (MQ=255)
                                                      cAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCa                                                                                                >  1:3631977/1‑150 (MQ=255)
                                                            tGCCGGGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTTATGACGTGGCGCGGCAGTTGGGTGGGTTCAGGCTCTCGTTCGCAGGGGCCGCACGGCGCCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCAc                                                                                          <  1:3551579/150‑1 (MQ=255)
                                                              ccGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCTGATGGGTGGGTTCAGGCTCTAGATCGCCGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGc                                                                                        <  1:1821985/150‑1 (MQ=255)
                                                              ccGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGc                                                                                        >  2:4318462/1‑150 (MQ=255)
                                                              ccGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGc                                                                                        >  2:4818810/1‑150 (MQ=255)
                                                               cgcgAGGAGTGCCAGGCGTTGCGTGGGTTGGCTCATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCa                                                                                       <  2:2463246/150‑1 (MQ=255)
                                                                 cgAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCAcc                                                                                     <  2:2772424/150‑1 (MQ=255)
                                                                 cgAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTATAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCAcc                                                                                     <  2:2772496/150‑1 (MQ=255)
                                                                    ggagTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCcgc                                                                                  >  2:2823560/1‑150 (MQ=255)
                                                                          ccAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGcttct                                                                            >  1:3571844/1‑150 (MQ=255)
                                                                            aGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCTGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCttcttc                                                                          <  1:5152159/150‑1 (MQ=255)
                                                                            aGGCGTGGGGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCttcttc                                                                          <  2:544814/150‑1 (MQ=255)
                                                                             ggCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTCGAGCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCg                                                                         <  1:5079060/150‑1 (MQ=255)
                                                                             ggCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCg                                                                         <  2:4094849/150‑1 (MQ=255)
                                                                             ggCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCg                                                                         <  1:5626460/150‑1 (MQ=255)
                                                                                  tGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATc                                                                    >  1:4123482/1‑150 (MQ=255)
                                                                                   gCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATcc                                                                   >  2:378139/1‑150 (MQ=255)
                                                                                   gCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATcc                                                                   >  1:2086303/1‑150 (MQ=255)
                                                                                      tGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCAc                                                                <  2:1939126/150‑1 (MQ=255)
                                                                                        ggTTGGCTTTTTCCCTGGCGCGGATGTGGGGTGGGTTCCGGCTCTCGATCGCTGGGGCCGCCGGGGGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGa                                                              <  1:545339/150‑1 (MQ=255)
                                                                                         gTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAg                                                             >  1:2535390/1‑150 (MQ=255)
                                                                                               tgatgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATg                                                       >  2:3184532/1‑150 (MQ=255)
                                                                                               tgatgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATg                                                       >  2:867081/1‑150 (MQ=255)
                                                                                               tgatgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATg                                                       >  2:2293501/1‑150 (MQ=255)
                                                                                                 atgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGaa                                                     <  2:638245/150‑1 (MQ=255)
                                                                                                 atgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGaa                                                     <  2:2754732/150‑1 (MQ=255)
                                                                                                 atgaCCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGaa                                                     <  2:4457925/150‑1 (MQ=255)
                                                                                                 atgaCCTGGCGCGGCAGATGGGTGGGTACAGGCTATAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGaa                                                     <  1:1318539/150‑1 (MQ=255)
                                                                                                     ccTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGatat                                                                                                         >  2:366359/1‑94 (MQ=255)
                                                                                                     ccTGGCGCGGCAGAGGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGatat                                                                                                         <  1:366359/94‑1 (MQ=255)
                                                                                                       tgggggggCAGATGGGTGGGTTCTGGCTCTTGTTCGCTGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgcgcgc                                               <  1:4421460/144‑1 (MQ=255)
                                                                                                       tGGCGCGGCTGTTGGGTGGTTTCTGGCTATATATGGCAGGGCCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgcgcgc                                               <  1:3349284/150‑1 (MQ=255)
                                                                                                       tGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgcgcgc                                               <  2:918144/150‑1 (MQ=255)
                                                                                                         gcgcGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAgcgcgcgc                                             >  1:219017/1‑150 (MQ=255)
                                                                                                             ggCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCAcc                                         >  2:2153227/1‑150 (MQ=255)
                                                                                                             ggCAGAGGGGTGGGTTCAGGCTCGAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCAcc                                         <  2:4045669/150‑1 (MQ=255)
                                                                                                              gctgttgggtgggTTCAGGCTCTAGAGCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCg                                        <  1:851604/145‑1 (MQ=255)
                                                                                                               cAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGc                                       >  2:3905881/1‑150 (MQ=255)
                                                                                                                tgATGGGTGGTTTCAGGCTATTGTTGGCTGGGGCCGCACGGCGGCCCCGTCTCTCTCTCAGCCACCAATGATCGTGCTGATATCCTCCGCCAGCTCACGCACCCGAGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCgat                                      <  2:1635369/149‑3 (MQ=255)
                                                                                                                   ggggtgggtTCCGGCTCTAGAGCGCCGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGcc                                   <  1:1425962/149‑1 (MQ=255)
                                                                                                                       tgggtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATa                               >  1:4059704/1‑150 (MQ=255)
                                                                                                                         ggtTCTGGCTCGAGAGCGCCGGGGCCGCAGGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATaaa                             <  1:1929385/150‑1 (MQ=255)
                                                                                                                          gtTCTGGCTCTTGATCGCTGGGGCCGCCGGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAg                            <  1:1111638/150‑1 (MQ=255)
                                                                                                                          gtTCCGGCTCTAGATCGCCGGGGCCGCAGGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAg                            <  1:1084403/150‑1 (MQ=255)
                                                                                                                          gtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATaaat                            <  1:5029069/150‑2 (MQ=255)
                                                                                                                          gtTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAg                            >  2:1052787/1‑150 (MQ=255)
                                                                                                                            tCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGt                          >  2:3510243/1‑150 (MQ=255)
                                                                                                                              aGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGtct                        >  2:5408939/1‑148 (MQ=255)
                                                                                                                              aGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGtat                        >  2:553321/1‑150 (MQ=255)
                                                                                                                                  tctAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATACAGGTATAGaa                    >  1:3101419/1‑150 (MQ=255)
                                                                                                                                    tAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAgc                  >  2:5051714/1‑150 (MQ=255)
                                                                                                                                        tCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCa              >  1:4922567/1‑150 (MQ=255)
                                                                                                                                           cAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGcc           >  1:2352864/1‑150 (MQ=255)
                                                                                                                                              gggCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCtt        >  1:2651168/1‑150 (MQ=255)
                                                                                                                                                gCCGCAGGGCGGCCCCGGCTCTCGCTCAGCCACCATTGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGc      <  1:5411351/150‑1 (MQ=255)
                                                                                                                                                gCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGc      >  2:4695831/1‑150 (MQ=255)
                                                                                                                                                 ccGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCAc                                                                <  2:356530/91‑1 (MQ=255)
                                                                                                                                                 ccGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGcc     <  1:4331751/150‑1 (MQ=255)
                                                                                                                                                  cGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGccc    >  1:3852381/1‑150 (MQ=255)
                                                                                                                                                  cGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGccc    <  1:2781044/150‑1 (MQ=255)
                                                                                                                                                  cGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGccc    <  2:1498711/150‑1 (MQ=255)
                                                                                                                                                   gCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACCTGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGcccc   >  2:2053382/1‑150 (MQ=255)
                                                                                                                                                   gCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGcccc   >  2:4931152/1‑150 (MQ=255)
                                                                                                                                                   gCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGcccc   >  2:4597100/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AACCGGACGGTGCAGGGGATTTCCGATTTGGCGCGTGAGACGGCGGCGGAGGTGCAAGGGTGCCGCGAGGAGTGCCAGGCGTTGCGTGGGTTGGCTGATGACCTGGCGCGGCAGATGGGTGGGTTCAGGCTCTAGATCGCAGGGGCCGCACGGCGGCCCCGGCTCTCGCTCAGCCACCAATGATCGTGCGGATATCCGCCGCCAGCTCACGCACCCGCGCTTCTTCGGTATCCCACGAGCACATGAAGCGCGCGCCACCGCTGCCGATAAAGGTATAGAAGCGCCAGCCCTTGCCCCG  >  NC_002947/385329‑385626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑