BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,084,378	G→A	6.7%	intergenic (‑3/+72)	PP_t55 ← / ← PP_t54	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,084,378	0	G	A	6.7%	430.1 / 13.3	148	intergenic (‑3/+72)	PP_t55/PP_t54	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base G (58/79); new base A (7/3); total (65/83)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

GTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTC  >  NC_002947/5084232‑5084525

gTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGAtt                                                                                                                                                  >  1:56516/1‑150 (MQ=255)

gTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGAtt                                                                                                                                                  >  1:56568/1‑150 (MQ=255)

gTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAtt                                                                                                                                                  <  1:4704666/150‑1 (MQ=255)

    ttttAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCa                                                                                                                                              >  1:291168/1‑150 (MQ=255)

      ttAGACCAGGTTACCCCTGGCTTACTGAACTGGCATTTCAAGCAGAACCAGTTCAAGCAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGt                                                                                                                                            <  2:5389722/150‑1 (MQ=14)

      atAGACCAGGTTACCACGGGCTTACTTACATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGt                                                                                                                                            <  2:5389833/149‑1 (MQ=255)

         gACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGtttt                                                                                                                                         >  2:317612/1‑150 (MQ=255)

          aCCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTa                                                                                                                                        >  2:2047853/1‑150 (MQ=255)

            cAGTTTACCCCTGGCTTACTGACCTGGCATTTCTAGCAGAACCACTTCAAGCCAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTaaa                                                                                                                                      <  2:793006/150‑1 (MQ=14)

            cAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATGGGCAGTTTTaaa                                                                                                                                      >  2:3421898/1‑150 (MQ=255)

            cAGGTTACCACTGGCTTACTGAAATGGATTTTCAAGCAGAACCACTTCTAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTaaa                                                                                                                                      <  1:152050/150‑1 (MQ=255)

              ggTTACCACTGGCTTACTGAAATGGCTTTTCTAGCAGAACCACTTCAAGACAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAAcc                                                                                                                                    <  2:803125/150‑1 (MQ=255)

               gTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCa                                                                                                                                   <  1:193352/150‑1 (MQ=255)

                 tACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGa                                                                                                                                 <  1:4963980/150‑1 (MQ=255)

                   ccACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGAtt                                                                                                                               >  1:3251367/1‑150 (MQ=255)

                    cACTGGCTTACTTAAATGGCTTTTCAAGCAGAACCACTTCAAGATAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTa                                                                                                                              <  2:1111819/150‑1 (MQ=255)

                    cACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTa                                                                                                                              >  2:3921157/1‑150 (MQ=255)

                    cACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGAtac                                                                                                                              >  2:4251537/1‑148 (MQ=12)

                    cACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGAtac                                                                                                                              >  2:976903/1‑148 (MQ=12)

                    cACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGAtac                                                                                                                              >  1:2126453/1‑148 (MQ=12)

                    cACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGAt                                                                                                                                >  1:506360/1‑148 (MQ=18)

                    cACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGAt                                                                                                                                <  2:506360/148‑1 (MQ=18)

                    cACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAAACCAGAtac                                                                                                                              >  1:3997770/1‑148 (MQ=37)

                     aCTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTAc                                                                                                                             >  2:5118750/1‑150 (MQ=255)

                          cTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTg                                                                                                                        <  1:1592705/150‑1 (MQ=255)

                          cTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTg                                                                                                                        <  1:1592694/150‑1 (MQ=255)

                           ttACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGTGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTgg                                                                                                                       >  1:4378096/1‑150 (MQ=255)

                            tACTGATATGTCTTTTCAAGCAGAACCACTTCTTGTTTGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGt                                                                                                                      <  2:3089530/150‑1 (MQ=14)

                            tACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGt                                                                                                                      <  1:317612/150‑1 (MQ=255)

                               tGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGa                                                                                                                   <  1:481545/150‑1 (MQ=255)

                               tGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGa                                                                                                                   <  1:481418/150‑1 (MQ=255)

                                gAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTa                                                                                                                                        >  1:2344836/1‑128 (MQ=35)

                                gAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTa                                                                                                                                        <  2:2344836/128‑1 (MQ=35)

                                 aaatcccTTTTAAAGAAGAAACAATTCAAGAAATTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACt                                                                                                                 <  2:1808646/144‑1 (MQ=14)

                                 aaaTGGCTTTTCAAGCAGAACCACTTCAAGACAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACt                                                                                                                 <  2:5451462/150‑1 (MQ=255)

                                 aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAg                                                                                                                                             >  2:2316903/1‑122 (MQ=35)

                                 aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTaaa                                                                                                                                      >  1:2138848/1‑129 (MQ=37)

                                 aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGaat                                                                                                                 >  1:3580726/1‑148 (MQ=255)

                                 aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACt                                                                                                                 <  2:1005525/150‑1 (MQ=255)

                                 aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACt                                                                                                                 <  1:1168922/150‑1 (MQ=255)

                                 aaaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGA‑TAACGCAGGTTggagt                                                                                                                >  2:2743722/1‑146 (MQ=11)

                                  aaTGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTg                                                                                                                <  2:3251367/150‑1 (MQ=255)

                                    tGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGaa                                                                                                              >  2:1310845/1‑150 (MQ=255)

                                     tgCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGaaa                                                                                                             <  2:4184006/149‑1 (MQ=255)

                                        ttttCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATgg                                                                                                          >  2:222555/1‑150 (MQ=255)

                                          ttCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCt                                                                                                        <  1:306992/150‑1 (MQ=255)

                                            cAAGCAGAACCTTTTCATGACTTTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCttt                                                                                                      <  2:5240683/150‑1 (MQ=17)

                                            cAAGCAGAACCTCTTCATGTCTCTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCttt                                                                                                      <  2:1760000/150‑1 (MQ=17)

                                            cAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCttt                                                                                                      <  1:2029542/150‑1 (MQ=255)

                                            cAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCttt                                                                                                      <  2:5241282/150‑1 (MQ=255)

                                                 aGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATAACCGATGGTTGAATGAAATGGCTTTGCTAg                                                                                                 >  2:3238791/1‑150 (MQ=255)

                                                         ttCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGa                                                                                                                                 <  2:1591117/110‑1 (MQ=35)

                                                         ttCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTGGACTGAAATGGCTTTGCAAGCAGAACCa                                                                                         >  2:1686756/1‑150 (MQ=255)

                                                             agctataggCGGACCCACCACGAATCCAACCATCCACCGCCCGGTTCGTACGCCACTAACCAAACCACCCGACCCATGACGACGCTATTGGCAGTTTTACACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTc                                                                                     <  2:5525690/143‑1 (MQ=255)

                                                             aGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTc                                                                                     <  1:539798/150‑1 (MQ=255)

                                                                 aGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAACCAGAACCACTTCAAGa                                                                                 <  2:1995610/150‑1 (MQ=255)

                                                                       ggACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTgg                                                                           <  1:43661/150‑1 (MQ=255)

                                                                       ggACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTAGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTgg                                                                           <  2:13170/150‑1 (MQ=255)

                                                                        gACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGc                                                                          <  1:323442/150‑1 (MQ=255)

                                                                         aCTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCg                                                                         <  1:3679762/150‑1 (MQ=255)

                                                                           tgagctgGAGTAGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGa                                                                       <  1:1307532/148‑1 (MQ=255)

                                                                           tCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTATACCAGATAACCGATGTTTGAATGAGATGGCTTTGAAAGAATAACAAGTAAAAGAAAGAGGCGGa                                                                       >  2:5199554/1‑150 (MQ=14)

                                                                               cattaTTCGAACCTTCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTca                                                                   <  1:1470690/146‑1 (MQ=255)

                                                                               cagGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTca                                                                   <  1:3576658/150‑1 (MQ=255)

                                                                               cagGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTca                                                                   <  1:3749484/150‑1 (MQ=255)

                                                                                 ggATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATAACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTcagc                                                                 >  2:1795139/1‑150 (MQ=255)

                                                                                  gATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTcagca                                                                <  1:2303684/150‑1 (MQ=255)

                                                                                       gAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACAACTTCAAGAAAGTGGCGGACTCGGCAGGAtg                                                           >  2:2128553/1‑149 (MQ=255)

                                                                                        aaCCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTc                                                          <  1:1111619/150‑1 (MQ=255)

                                                                                        aaCCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTc                                                          <  2:2715168/150‑1 (MQ=255)

                                                                                        aaCCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAAAGGCTTTGCAAGCAGAACCACTTCAAGAAGGTGGCGGACTCAGCAGGATTc                                                          >  2:3503907/1‑150 (MQ=255)

                                                                                            tGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAAc                                                      >  1:2384347/1‑150 (MQ=255)

                                                                                                  cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTg                                                                                                                                                 >  2:1042163/1‑53 (MQ=35)

                                                                                                  cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGa                                                <  2:1641329/150‑1 (MQ=255)

                                                                                                  cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGa                                                >  1:3908167/1‑150 (MQ=255)

                                                                                                  cGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGa                                                >  1:3907963/1‑150 (MQ=255)

                                                                                                    cTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGAGGACTCAGCAGGATTCGAACCTGCGAcc                                              >  2:4576235/1‑150 (MQ=255)

                                                                                                        gTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTc                                          >  1:536864/1‑150 (MQ=255)

                                                                                                             gaGCCGTGTACTCGATACAGCTGATCTATGTGTCCGCGTTTGGCAGTTTTATACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTc                                     <  2:5155391/149‑1 (MQ=17)

                                                                                                              aGCCGAGTACTCTATCCAGCTGAGCTTTGAGTCCGCGTTTGGCAGTTTTACACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCg                                    <  2:2522330/150‑1 (MQ=255)

                                                                                                               gCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGt                                   >  1:3372637/1‑150 (MQ=255)

                                                                                                                ccGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTa                                  <  1:3136234/150‑1 (MQ=255)

                                                                                                                     tACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa                             <  1:1310845/150‑1 (MQ=255)

                                                                                                                     tACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa                             <  2:56516/150‑1 (MQ=255)

                                                                                                                     tACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa                             <  2:56568/150‑1 (MQ=255)

                                                                                                                        tctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCGAGAAAGTGGCGGACTCAGCAGGATTCGAACCTg                                                   >  2:3150751/1‑125 (MQ=255)

                                                                                                                        tctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAATAGTGTGGCGGACTCACCAGGATTAGAAGCTGCGACCGCGCTTGTGTTAGCCGAGTa                          >  2:4293200/1‑150 (MQ=17)

                                                                                                                        tctATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTa                          >  1:1943875/1‑150 (MQ=255)

                                                                                                                          tATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTAct                        >  1:4686389/1‑150 (MQ=255)

                                                                                                                                   gAGCTTTGTTTCCGCGATTGGCAGTTTTACACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc               <  2:4053344/150‑1 (MQ=255)

                                                                                                                                      ccaTGAGTCCGCGATTGGCAGTTTTAACCCAGATTTCCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGa            <  2:4378096/148‑1 (MQ=255)

                                                                                                                                       tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg           >  1:3268551/1‑150 (MQ=255)

                                                                                                                                       tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGAACTCTATCCAGCTGAg           >  1:3847113/1‑150 (MQ=255)

                                                                                                                                       tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGAACTCTATACAGCTGAg           >  1:3847105/1‑150 (MQ=255)

                                                                                                                                        aTGTGTCCGCGTTTGGCAGTTTTACACCAGTTTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc          <  2:3970502/150‑1 (MQ=255)

                                                                                                                                           aGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTAt       <  2:2519210/150‑1 (MQ=255)

                                                                                                                                             tCCGCGATTGGCAGTTTTACACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa     <  2:291168/150‑1 (MQ=255)

                                                                                                                                             tCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGa     >  1:2892250/1‑150 (MQ=255)

                                                                                                                                               cgcgATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGt   >  1:3212021/1‑150 (MQ=255)

                                                                                                                                                gcgATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTc  >  1:3988255/1‑150 (MQ=255)

GTAGTTTTAGACCAGGTTACCACTGGCTTACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTC  >  NC_002947/5084232‑5084525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`